High-Throughput Construction of Genetically Modified Fungi

2016 ◽  
pp. 241-252 ◽  
Author(s):  
Gyungsoon Park ◽  
Shouqiang Ouyang ◽  
Katherine A. Borkovich
Keyword(s):  
High Throughput ◽  
Genetically Modified

scholarly journals High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days

2012 ◽  
Vol 78 (12) ◽  
pp. 4217-4224 ◽  
Author(s):  
Stefan G. Buzoianu ◽  
Maria C. Walsh ◽  
Mary C. Rea ◽  
Orla O'Sullivan ◽  
Paul D. Cotter ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Intestinal Microbiota ◽  
High Throughput ◽  
Starch Content ◽  
Genetically Modified ◽  
Human Model ◽  
Rrna Gene ◽  
Bt Maize ◽  
Content Type ◽  
Mon810 Maize

ABSTRACTThe objective of this study was to investigate if feeding genetically modified (GM) MON810 maize expressing theBacillus thuringiensisinsecticidal protein (Bt maize) had any effects on the porcine intestinal microbiota. Eighteen pigs were weaned at ∼28 days and, following a 6-day acclimatization period, were assigned to diets containing either GM (Bt MON810) maize or non-GM isogenic parent line maize for 31 days (n= 9/treatment). Effects on the porcine intestinal microbiota were assessed through culture-dependent and -independent approaches. Fecal, cecal, and ileal counts of total anaerobes,Enterobacteriaceae, andLactobacilluswere not significantly different between pigs fed the isogenic or Bt maize-based diets. Furthermore, high-throughput 16S rRNA gene sequencing revealed few differences in the compositions of the cecal microbiotas. The only differences were that pigs fed the Bt maize diet had higher cecal abundance ofEnterococcaceae(0.06 versus 0%;P< 0.05),Erysipelotrichaceae(1.28 versus 1.17%;P< 0.05), andBifidobacterium(0.04 versus 0%;P< 0.05) and lower abundance ofBlautia(0.23 versus 0.40%;P< 0.05) than pigs fed the isogenic maize diet. A lower enzyme-resistant starch content in the Bt maize, which is most likely a result of normal variation and not due to the genetic modification, may account for some of the differences observed within the cecal microbiotas. These results indicate that Bt maize is well tolerated by the porcine intestinal microbiota and provide additional data for safety assessment of Bt maize. Furthermore, these data can potentially be extrapolated to humans, considering the suitability of pigs as a human model.

High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp.

2007 ◽  
Vol 1139 (2) ◽  
pp. 214-220 ◽  
Author(s):  
Jana Olšovská ◽  
Markéta Jelínková ◽  
Petr Man ◽  
Markéta Koběrská ◽  
Jiří Janata ◽  
...  
Keyword(s):  
High Throughput ◽  
Fermentation Broth ◽  
Genetically Modified ◽  
Streptomyces Spp

A high-throughput multiplex tandem PCR assay for the screening of genetically modified maize

LWT ◽  
2018 ◽  
Vol 87 ◽  
pp. 169-176 ◽  
Author(s):  
Shuang Wei ◽  
Chenguang Wang ◽  
Pengyu Zhu ◽  
Guangbiao Zhou ◽  
Wei Fu ◽  
...  
Keyword(s):  
High Throughput ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Pcr Assay

scholarly journals MACRO: A Combined Microchip-PCR and Microarray System for High-Throughput Monitoring of Genetically Modified Organisms

2014 ◽  
Vol 86 (2) ◽  
pp. 1269-1276 ◽  
Author(s):  
Ning Shao ◽  
Shi-Meng Jiang ◽  
Miao Zhang ◽  
Jing Wang ◽  
Shu-Juan Guo ◽  
...  
Keyword(s):  
High Throughput ◽  
Genetically Modified Organisms ◽  
Genetically Modified
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