Plant Efficacy and Alkaloids Production in Sadabahar (Catharanthus roseus L.): Role of Potent PGRs and Mineral Nutrients

2017 ◽  
pp. 35-57
Author(s):  
M. Naeem ◽  
Tariq Aftab ◽  
Mohd. Idrees ◽  
M. Masidur Alam ◽  
M. Masroor A. Khan ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Mineral Nutrients

Characterization of Hexokinase and Fructokinase from Suspension-Cultured Catharanthus roseus Cells

1988 ◽  
Vol 43 (11-12) ◽  
pp. 827-834 ◽  
Author(s):  
Yüko Yamashita ◽  
Hiroshi Ashihara
Keyword(s):  
Catharanthus Roseus ◽  
Divalent Cations ◽  
Ph Optimum ◽  
Optimum Ph ◽  
Phosphate Donor

Abstract Two different hexose-phosphorylating enzymes, hexokinase and fructokinase, were partially purified from suspension-cultured Catharanthus roseus cells. One of the enzymes, hexokinase, catalyzed the phosphorylation of both glucose and fructose. The Km values for glucose and fructose were 0.06 mM and 0.23 mM, respectively. The Vmax of the enzyme with fructose was approximately three times higher than with glucose. This enzyme was specific in its requirement for ATP and its Km value for ATP was 52 μM. The optimum pH was 8.0 and Mg2+ or Mn2+ was required for the activity. The activity was inhibited by considerably higher concentrations of ADP (i.e., 4 mM ADP was required for 50% inhibition). The second enzyme, fructokinase, was specific for fructose, and no activity was detected with glucose as substrate. This enzyme used UTP or CTP as phosphate donor. The Km values of this enzyme for fructose and UTP were 0.13 mM and 0.15 mM, respectively. The pH optimum was 7.2, and Mg2+ or Mn2+ was required for the activity. These divalent cations could be partially replaced by Ca2+. The activity was inhibited noncompetitively by ADP and AMP. 90% inhibition of the activity by 0.5 mM ADP was observed in the presence of 2 mM UTP and 5 mM MgCl2. Fructose-2,6-bisphosphate, glucose-1,6-bisphosphate, glucose-6-phosphate, and fructose-6-phosphate had little or no effect on the activity of both the hexokinase and the fructokinase. Based on these results, a discussion is presented of the role of hexokinase and fructokinase and their involvement in the regulation of the metabolism of sugars in Catharanthus cells.

Possible role of adenylates in the engagement of the cyanide-resistant pathway in nutrient-starved Catharanthus roseus cells

1994 ◽  
Vol 90 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Marcel H. N. Hoefnagel ◽  
Frank Van Iren ◽  
Kees R. Libbenga ◽  
Linus H. W. Van der Plas
Keyword(s):  
Catharanthus Roseus

scholarly journals A Review on Asymbiotic Seed Germination in Orchids through Plant Tissue Culture

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Purnima Paramanik ◽  
◽  
Dipak Kar ◽  
Subrata Raha ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Mineral Nutrients ◽  
Symbiotic Association ◽  
Organic Additives ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Mycorrhizal Association ◽  
Commercially Important

Orchid seeds are very small, dust like in appearance, fusiform in shape, lacking endosperm and have undifferentiated embryo covered by transparent seed coat. Mycorrhizal association is required for seed germination of naturally growing orchids. In this symbiotic association, orchid species are dependent on mycorrhizal association for supply of mineral nutrients. In in-vitro condition, such demand of minerals may be compensated by external supply of sugar and mineral nutrients that are required for seed germination of orchid. Several orchids are responded by this asymbiotic seed culture and have commercial importance. Therefore, formulation of efficient in-vitro protocol is important for commercially important orchid species as well as endangered orchid species for conservation. This review paper is focused on various aspects of asymbiotic seed germination of orchids and the role of organic additives in successful seed germination.

Regulatory Role of Mineral Nutrients in Nurturing of Medicinal Legumes Under Salt Stress

2017 ◽  
pp. 309-334 ◽  
Author(s):  
M. Naeem ◽  
Abid A. Ansari ◽  
Sarvajeet Singh Gill ◽  
Tariq Aftab ◽  
Mohd. Idrees ◽  
...  
Keyword(s):  
Salt Stress ◽  
Mineral Nutrients ◽  
Regulatory Role
Sign in / Sign up

Export Citation Format

Share Document