scholarly journals Genotoxic potential of Bohadschia vitiensis aqueous extract: A sea cucumber species, using Allium cepa genotoxicity model

2021 ◽  
Vol 6 (1) ◽  
pp. 9-14
Author(s):  
Nishani J. Jayathilake ◽  
Uthpala A. Jayewardena
Keyword(s):  
Allium Cepa ◽  
Aqueous Extract ◽  
Sea Cucumber ◽  
Genotoxic Potential

Allium cepa Root Chromosomal Aberration Assay: An Application in Assessing Anti-genotoxic Potential of Ashwagandha

2017 ◽  
pp. 69-79
Author(s):  
Rajneet Kour Soodan ◽  
Ashita Sharma ◽  
Mandeep Kaur ◽  
Jatinder Kaur Katnoria ◽  
Avinash Kaur Nagpal
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Genotoxic Potential ◽  
Chromosomal Aberration Assay

In vitro evaluation of antimicrobial activity and measuring its antigenic specific immune response in mice model studies: Allium cepa

2021 ◽  
pp. 2563-2568
Author(s):  
Amit Gupta ◽  
Karishma Ghosh ◽  
Dakshita Snud Sharma ◽  
Shubham Tyagi
Keyword(s):  
Cell Proliferation ◽  
Antimicrobial Activity ◽  
T Cell ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Hemolytic Activity ◽  
Cell Culture Supernatant ◽  
T Cell Proliferation ◽  
Mice Model ◽  
Model Studies

Allium cepa (onion; Liliaceae family), herbaceous annual plant and reported Sulphur based amino acids with many minerals including vitamins. Inspite of various medicinal uses of this plant as food and also showed various beneficial effects. The antimicrobial activity of aqueous extract from Allium cepa (onion bulb) was evaluated against bacterial strains using disc diffusion method. In contrast, inflammatory response or inhibition of T cell antigenic response was evaluated using typhoid vaccine and swine flu vaccine in Swiss mice. These studies were conducted according to ethical guidelines and determining T cell proliferation including estimation of cytokines from cell culture supernatant and also measuring its hemolytic activity. The results showed that aqueous extract show the highest inhibition rate against Pseudomonas aeruginosa and Salmonella enteritidis. In mice model studies, aqueous extract at higher doses showed inhibition in antigen specific T cell proliferation and also declining in the level of IFN-gamma production but enhancement in IL-4 production. In addition, aqueous extract at higher concentration does not showed any hemolytic activity. Overall, this study provides evidence for the presence of secondary metabolites in aqueous extract that probably interferes with bacterial growth (antimicrobial) and also declining in antigenic specific proliferation (i.e. anti-inflammatory) which would prevent and inhibit initiation and progression of many diseases.

Assessment of antimutagenic and genotoxic potential of senna (Cassia angustifolia Vahl.) aqueous extract using in vitro assays

2008 ◽  
Vol 22 (1) ◽  
pp. 212-218 ◽  
Author(s):  
C.R. Silva ◽  
M.R. Monteiro ◽  
H.M. Rocha ◽  
A.F. Ribeiro ◽  
A. Caldeira-de-Araujo ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
In Vitro Assays ◽  
Cassia Angustifolia ◽  
Genotoxic Potential

scholarly journals Cytotoxic and micronuclei inducing effects of petroleum ether fraction of leaf aqueous extract of Clerodendrum viscosum Vent. in Allium cepa root tip cells

2021 ◽  
Author(s):  
Sujit Roy ◽  
Lalit Mohan Kundu ◽  
Gobinda Chandra Roy ◽  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Petroleum Ether ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Root Tip ◽  
Petroleum Ether Fraction ◽  
Leaf Aqueous Extract ◽  
Ether Fraction ◽  
Clerodendrum Viscosum ◽  
Tip Cells ◽  
Root Tip Cells

Clerodendrum viscosum is a traditionally used medicinal plant. The present study aimed to analyze a detailed cytotoxic effect of the nonpolar petroleum ether fraction (AQPEF) of leaf aqueous extract of C. viscosum Vent. (LAECV) in Allium cepa root tip cells. The LAECV was fractionated with petroleum ether and tested for A. cepa toxicity at early hours (2 and 4 h treatment) at concentrations 0, 0.050, 0.100, 0.150 and 0.200 mg mL-1. The highest aberrant cell percentage (10.45%) was scored from 0.150 mg mL-1 followed by0.100 mg mL-1 (8.75%) concentration at 4 h treated samples. The AQPEF treatment induced a significant (p<0.0001) increase in micronuclei frequency; 4.31, 5.08, 5.05 and 3.05% respectively at concentrations 0.50, 0.100, 0.150, and 0.200 mg mL-1. The highest polyploid frequency (20.14%) induced with 0.100 mg mL-1 of AQPEF at 16 h recovery. 0.150 mg mL-1 is the most effective concentration of AQPEF to decipher its activity similar to colchicine (0.150 mg mL-1). In summary, the present study indicates petroleum ether is suitable for extraction of the active phytochemicals of LAECV having cytotoxic effects on A. cepa root tip cells. The AQPEF has colchicine like micronuclei, polyploidy, and mitotic abnormality inducing potentials in A. cepa root apical meristem cells.

scholarly journals Antibacterial effect of Allium cepa (onion), Allium sativum (garlic), Azadirachta indica (neem) extract against different bacteria isolated from clinical sample.

2021 ◽  
Vol 28 (08) ◽  
pp. 1083-1089
Author(s):  
Muhammad Shahbaz Hussain ◽  
◽  
Muhammad Bilal Ghafoor ◽  
Umar Khalid Cheema ◽  
Muhammad Sajjad ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Aqueous Extract ◽  
Azadirachta Indica ◽  
Allium Sativum ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Neem Extract ◽  
Gram Negative

Objective: The objective of this study was to evaluate and screen the antibacterial activity of medicinal plant which were most cultivated in ancient time. Study Design: Experimental Study. Setting: Microbiology Section, Pathology Department, Sheikh Zayed Hospital/Medical College Rahim Yar Khan. Period: February 2019 to May 2019. Material & Methods: The fresh aqueous extract of garlic cloves (Allium sativum), onion bulb (Allium cepa) and neem (Azadirachta indica) leaves used against the clinically isolated Staphylococcus aureus(S.aureus), Escherichia coli (E.coli), Klebsiella, Acinetobacter and Pseudomonas species by using agar well diffusion method. Aqueous extract of used plant tested at different concentrations of 100%, 75%, 50%, 25%. Results: All these concentrations showed inhibitory effect towards Gram positive bacteria (S.aureus), while Gram negative bacteria more sensitive to higher concentration. In this analysis, on comparison we observed that fresh garlic juice extract was more efficient on all tested bacteria either Gram positive or Gram negative than other and give maximum zone of inhibition on S.aureus (40mm) due to their high chemistry. Gram negative bacteria mainly E. coli somehow resistant to onion and neem extract because they possess the ability to rapidly mutate their genetic makeup towards phytochemical compounds. Conclusion: The result of study also elicits that efficacy of fresh extract depends upon concentrations levels. The plant showed maximum zone of inhibition at 100% than at 25% because on higher level they contain higher bioactive compounds.

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