DNA Polymorphism and Genetic Diversity in Raphanus Accessions

2017 ◽  
pp. 71-91
Author(s):  
Li Zhang ◽  
Qingbiao Wang
Keyword(s):  
Genetic Diversity ◽  
Dna Polymorphism

scholarly journals Characterization of Natural Isolates of Bipolaris maydis Associated with Mating Types, Genetic Diversity, and Pathogenicity in Fujian Province, China

Plant Disease ◽  
2020 ◽  
Vol 104 (2) ◽  
pp. 323-329
Author(s):  
Yuli Dai ◽  
Lin Gan ◽  
Hongchun Ruan ◽  
Niuniu Shi ◽  
Yixin Du ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mating Type ◽  
Dna Polymorphism ◽  
Sweet Corn ◽  
Issr Markers ◽  
Bipolaris Maydis ◽  
Fujian Province ◽  
Field Isolates ◽  
Rational Distribution

Due to the natural destructiveness and persistence of the southern corn leaf blight (SCLB) fungus Bipolaris maydis (Nisikado et Miyake) Shoem, the characterization of B. maydis field isolates is essential to guide the rational distribution of resistant materials in corn-growing regions. In the present study, 102 field isolates collected from seven locations covering the entire region of Fujian Province, China, were assessed for mating type distribution, genetic diversity, and pathogenicity toward local sweet corn cultivars. Mating type detection via polymerase chain reaction indicated that 36.3 and 63.7% of isolates were MAT1-1 and MAT1-2, respectively; more than 80% of these isolates were confirmed using cross assays with known mating type isolates. Thirteen intersimple sequence repeat (ISSR) markers within and among two mating type populations revealed a high level of DNA polymorphism for all combined isolates and between MAT1-1 and MAT1-2 populations. The MAT1-2 population was more diverse based on DNA polymorphism than the MAT1-1 population. The value of GST was 0.0070, ranging from 0.0399 to 0.3044 based on analysis of combined isolates and individual regional populations, respectively, suggesting the presence of genetic differentiation in the two mating type populations from different locations. Pathogenicity assays revealed that both MAT1-1 and MAT1-2 populations were pathogenic to all 11 local sweet corn cultivars tested in this study. The potential of sexual reproduction, existence of genetic diversity in the two mating type populations, and pathogenicity suggest that B. maydis populations have independently clonally adapted under natural field conditions during corn cultivation.

Investigation of IRAP transposon-based molecular markers for analysis of genetic diversity in pea germplasm

2018 ◽  
Author(s):  
Sajjad Ahmad ◽  
Rajvinder Kaur ◽  
Mark Lefsrud ◽  
Jaswinder Singh
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Dna Polymorphism ◽  
Breeding Programs ◽  
Molecular Analyses ◽  
Marker System ◽  
Varietal Identification ◽  
D Values ◽  
Mean Similarity ◽  
Germplasm Accessions

Retrotransposons diversity has been extensively studied in monocots, but it is not well documented in dicot species. Transposition activity of transposons creates DNA polymorphism and their abundant presence in genomes is making transposons a promising marker system for varietal identification and fingerprinting. In this study, four transposon-based markers (two DNA- and two RNA-transposons) were employed to evaluate the effectiveness of Inter-Retrotransposon Amplified Polymorphism (IRAP) transposon system in assessing genetic diversity in pea germplasm accessions. A total of 28 alleles were detected across the 35 pea accessions with number of alleles per locus ranged from 5 (Mutator) to 9 (Cyclops). RNA transposons produced a higher number of polymorphic alleles (Ogre: 8, Cyclops: 9) than DNA transposon markers (Mutator: 5, MITE: 6). Overall mean PIC value and D values for these transposon markers were 0.810 and 0.817 respectively. Genetic similarity values ranged from 0.143 to 0.823 with a mean similarity value of 0.403. Cluster analysis classified pea genotypes into six major groups that were somewhat consistent with their geographical origins. The molecular analyses differentiated all the 35 accessions and generated higher PIC and D values that can be useful for MAS-based breeding programs in pea.

scholarly journals Using Fitness Value for Monitoring Kiwifruit’s Variant Seedling in Tissue Culture

2016 ◽  
Vol 16 (5) ◽  
pp. 59-68
Author(s):  
Shouguo Tang ◽  
Yong Li ◽  
Zhikun Zhang
Keyword(s):  
Genetic Algorithm ◽  
Genetic Diversity ◽  
Pattern Recognition ◽  
Tissue Culture ◽  
Genetic Stability ◽  
Genomic Dna ◽  
Dna Polymorphism ◽  
Fitness Function ◽  
Fitness Value ◽  
Pattern Recognition Approach

Abstract Based on Genetic Algorithm, a pattern recognition approach using fitness to dynamically monitor the sub cultured seeding of kiwifruit is proposed in order to decrease the loss of variant seedlings in tissue culture. By coding, selection, mutation and cross-overing the selected primer pairs of the sub cultured seeding, we simulate the process of optimizing the kiwifruit’s genomic DNA polymorphism. The corresponding fitness values of the primer pairs are evaluated with fitness function for monitor the variation of kiwi’s DNA. The result shows that kiwi’s plantlets can better maintain their genes’ genetic stability for the first to the ninth generation. But from the tenth generation, the fitness values become variation. The results are based on experimentation, which uses optimized AFLP system for analyzing genetic diversity of 75 samples of seventh to eleventh 5 generations of kiwi.

The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat

2000 ◽  
Vol 100 (3-4) ◽  
pp. 584-592 ◽  
Author(s):  
M. Prasad ◽  
R. K. Varshney ◽  
H. S. Balyan ◽  
P. K. Gupta ◽  
J. K. Roy
Keyword(s):  
Genetic Diversity ◽  
Dna Polymorphism ◽  
Genotype Identification

Study on genetic diversity of Castanopsis phuthoensis Luong in Phu Tho province, Vietnam by RAPD markers

2016 ◽  
Vol 14 (2) ◽  
pp. 237-243
Author(s):  
Nguyễn Văn Thọ ◽  
Lê Thị Mai Linh ◽  
Nguyễn Viễn ◽  
Phạm Quang Tiến
Keyword(s):  
Genetic Diversity ◽  
Tree Species ◽  
Dna Polymorphism ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Analysis ◽  
Dna Fragments ◽  
Similarity Coefficients ◽  
Rapd Pcr ◽  
Rapd Primer

Castanopsis phuthoensis Luong is an endemic tree species of Phu Tho province, only distributed narrowly in forest rehabilitation in two communes belonging to Doan Hung district with density of this species is very low, only from 3.1 to 11.1 trees per hectare. Diameter distributions of the species of number of trees is characterized by curve style with a peak in 20cm or 24cm diameter classes. As it is difficult to find the seedling in the nature, research on forest structure, relations between tree species and genetic diversity is very necessary to define method of conservation for this species. In this study, we used RAPD markers to study on genetic diversity of Castanopsis phuthoensis Luong. The random amplified polymorphic DNA (RAPD) based on the polymeraze chain reaction (PCR) detects nucleotide sequence polymorphism using a single 10mer of arbitrary nucleotided sequence. The RAPD technology has quickly gained widespread acceptance and application because it providesa tool for genetic analysis that have not previously benefited the use of molecular markers. In this study, ten random primers to analyse the genetic diversity of 15 Castanopsis phuthoensis Luong samples that were collected from Phu Tho province, Vietnam. 9 RAPD primers gave DNA polymorphism and 01 RAPD primer OPA20 gave not DNA polymorphism. In the analysis region 0.25-2 kb, there were 56 DNA fragments amplified and 34 DNA fragments were polymorphic. Genetic similarity coefficients of 15 Castanopsis phuthoensis Luong samples ranged from 0.55- 0.95. The phylogenetic tree of 15 samples are divided into two main groups. As results of RAPD-PCR analysis, these samples were collected from the homologous geographical locations and the genetic diversity of 15 samples is not high. Therefore, it is necessary to conserve the "Castanopsis phuthoensis Luong".

scholarly journals Genetic Diversity and Dynamics of Sinorhizobium meliloti Populations Nodulating Different Alfalfa Cultivars in Italian Soils

2000 ◽  
Vol 66 (11) ◽  
pp. 4785-4789 ◽  
Author(s):  
Maria Carelli ◽  
Stefano Gnocchi ◽  
Silvia Fancelli ◽  
Alessio Mengoni ◽  
Donatella Paffetti ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Polymorphism ◽  
Genetic Structure ◽  
Sinorhizobium Meliloti ◽  
Dna Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Analysis Of Molecular Variance ◽  
Molecular Variance ◽  
High Level ◽  
Rhizobium Population

ABSTRACT We analyzed the genetic diversity of 531 Sinorhizobium meliloti strains isolated from nodules of Medicago sativa cultivars in two different Italian soils during 4 years of plant growth. The isolates were analyzed for DNA polymorphism with the random amplified polymorphic DNA method. The populations showed a high level of genetic polymorphism distributed throughout all the isolates, with 440 different haplotypes. Analysis of molecular variance allowed us to relate the genetic structure of the symbiotic population to various factors, including soil type, alfalfa cultivar, individual plants within a cultivar, and time. Some of these factors significantly affected the genetic structure of the population, and their relative influence changed with time. At the beginning of the experiment, the soil of origin and, even more, the cultivar significantly influenced the distribution of genetic variability of S. meliloti. After 3 years, the rhizobium population was altered; it showed a genetic structure based mainly on differences among plants, while the effects of soil and cultivar were not significant.

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