Polysaccharide Production by Grapes Must and Wine Microorganisms

2017 ◽  
pp. 293-314 ◽  
Author(s):  
Maria Dimopoulou ◽  
Aline Lonvaud-Funel ◽  
Marguerite Dols-Lafargue
Keyword(s):  
Polysaccharide Production

Investigation of culture conditions by Response Surface Methodology and kinetic modeling for exopolysaccharide production by Klebsiella oxytoca ICCF 419 strain, using lactose as substrate

2020 ◽  
Vol 25 (6) ◽  
pp. 2033-2044
Author(s):  
CATERINA TOMULESCU ◽  
◽  
MIŞU MOSCOVICI ◽  
ROXANA MĂDĂLINA STOICA ◽  
GABRIEL BUJOR ALBU ◽  
...  
Keyword(s):  
Citric Acid ◽  
Klebsiella Oxytoca ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Biomass Growth ◽  
Initial Value ◽  
Substrate Consumption ◽  
Polysaccharide Production ◽  
Crude Polysaccharide ◽  
Central Composite

The objective of this work was to optimize the bioprocess parameters, using Klebsiella oxytoca ICCF 419 to obtain an exopolysaccharide based on lactose as substrate. A kinetic study was employed and Logistic and Gompertz models were applied to describe the polysaccharide production, in relation with biomass growth and substrate consumption. The RSM methodology based on Central Composite Rotatable Design was used to evaluate and optimize the effect of lactose, corn extract, KH2PO4 and citric acid concentrations as independent variables on the polysaccharide production, biomass growth and substrate consumption as the response functions. The interaction effects and optimal parameters were obtained using Design Expert Software (version 9.0.6.2). The significance of the variables and their interactions was tested by means of ANOVA analysis with a 99% confidence level. The optimum culture conditions were determined and the model prediction was compared with experimental results. At an initial value of 23.45 for the C/N in the fermentation medium, the strain produces 17.41 g/L of crude polysaccharide and 2.53 g/L dry biomass. The EPS production was significantly influenced by lactose, corn extract and KH2PO4, while the citric acid had no influence. The biomass growth was influenced by the corn extract, KH2PO4 and citric acid.

scholarly journals Effect of sodium and calcium on polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Lentinus edodes

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bilal Adil ◽  
Quanju Xiang ◽  
Maolan He ◽  
Yuetong Wu ◽  
Muhammad Ahsan Asghar ◽  
...  
Keyword(s):  
Lentinus Edodes ◽  
Polysaccharide Production ◽  
Polysaccharide Synthesis

scholarly journals Acidic polysaccharide production from methane by a new methane-oxidizing bacterium H-2.

1983 ◽  
Vol 47 (2) ◽  
pp. 275-280 ◽  
Author(s):  
Kazuhiro CHIDA ◽  
Gwo-Jenn SHEN ◽  
Tohru KODAMA ◽  
Yasuji MINODA
Keyword(s):  
Acidic Polysaccharide ◽  
Polysaccharide Production

Polysaccharide production by kefir grains during whey fermentation

2001 ◽  
Vol 68 (4) ◽  
pp. 653-661 ◽  
Author(s):  
PABLO SEBASTIÁN RIMADA ◽  
ANALÍA GRACIELA ABRAHAM
Keyword(s):  
Soluble Fraction ◽  
Water Soluble ◽  
Grain Weight ◽  
Fermentation Time ◽  
Polysaccharide Fraction ◽  
Polysaccharide Production ◽  
Lactose Concentration ◽  
The Media ◽  
Soluble Polysaccharide ◽  
Kefir Grains

Fermentation of deproteinised whey with kefir grains CIDCA AGK1 was studied focusing on polysaccharide production from lactose. Kefir grains were able to acidify whey at different rates depending on the grain/whey ratio. During fermentation, kefir grains increased their weight and a water-soluble polysaccharide was released to the media. Exopolysaccharide concentration increased with fermentation time, reaching values of 57·2 and 103·4 mg/l after 5 days of fermentation in cultures with 10 and 100 g kefir grains/l, respectively. The polysaccharide fraction quantified after fermentation corresponded to the soluble fraction, because part of the polysaccharide became a component of the grain. Weight of kefir grains varied depending on the time of fermentation. Polysaccharide production was affected by temperature. Although the highest concentration of polysaccharide in the media was observed at 43 °C at both grain/whey ratios, the weight of the grains decreased in these conditions. In conclusion, kefir grains were able to acidify deproteinised whey, reducing lactose concentration, increasing their weight and producing a soluble polysaccharide.

Porphyra cell cultures: isolation, growth and polysaccharide production

1990 ◽  
Vol 2 (1) ◽  
pp. 63-70 ◽  
Author(s):  
M. I. Tait ◽  
A. M. Milne ◽  
D. Grant ◽  
J. A. Somers ◽  
J. Staples ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Polysaccharide Production
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