Yeast Viability and Vitality

2017 ◽  
pp. 147-165 ◽  
Author(s):  
Graham G. Stewart
Keyword(s):  
Yeast Viability

scholarly journals Distinct Roles for the Yeast Phosphatidylinositol 4-Kinases, Stt4p and Pik1p, in Secretion, Cell Growth, and Organelle Membrane Dynamics

2000 ◽  
Vol 11 (8) ◽  
pp. 2673-2689 ◽  
Author(s):  
Anjon Audhya ◽  
Michelangelo Foti ◽  
Scott D. Emr
Keyword(s):  
Cell Growth ◽  
Membrane Trafficking ◽  
Secretory Pathway ◽  
Membrane Dynamics ◽  
Small Gtpase ◽  
Effector Proteins ◽  
Restrictive Temperature ◽  
Yeast Saccharomyces Cerevisiae ◽  
Cytoskeleton Organization ◽  
Yeast Viability

The yeast Saccharomyces cerevisiae possesses two genes that encode phosphatidylinositol (PtdIns) 4-kinases,STT4 and PIK1. Both gene products phosphorylate PtdIns at the D-4 position of the inositol ring to generate PtdIns(4)P, which plays an essential role in yeast viability because deletion of either STT4 orPIK1 is lethal. Furthermore, although both enzymes have the same biochemical activity, increased expression of either kinase cannot compensate for the loss of the other, suggesting that these kinases regulate distinct intracellular functions, each of which is required for yeast cell growth. By the construction of temperature-conditional single and double mutants, we have found that Stt4p activity is required for the maintenance of vacuole morphology, cell wall integrity, and actin cytoskeleton organization. In contrast, Pik1p is essential for normal secretion, Golgi and vacuole membrane dynamics, and endocytosis. Strikingly,pik1tscells exhibit a rapid defect in secretion of Golgi-modified secretory pathway cargos, Hsp150p and invertase, whereas stt4tscells exhibit no detectable secretory defects. Both single mutants reduce PtdIns(4)P by ∼50%; however,stt4ts/pik1tsdouble mutant cells produce more than 10-fold less PtdIns(4)P as well as PtdIns(4,5)P2. The aberrant Golgi morphology found in pik1tsmutants is strikingly similar to that found in cells lacking the function of Arf1p, a small GTPase that is known to regulate multiple membrane trafficking events throughout the cell. Consistent with this observation, arf1 mutants exhibit reduced PtdIns(4)P levels. In contrast, diminished levels of PtdIns(4)P observed in stt4tscells at restrictive temperature result in a dramatic change in vacuole size compared with pik1tscells and persistent actin delocalization. Based on these results, we propose that Stt4p and Pik1p act as the major, if not the only, PtdIns 4-kinases in yeast and produce distinct pools of PtdIns(4)P and PtdIns(4,5)P2that act on different intracellular membranes to recruit or activate as yet uncharacterized effector proteins.

Flow cytometry and cell sorting for yeast viability assessment and cell selection

Yeast ◽  
1998 ◽  
Vol 14 (2) ◽  
pp. 147-160 ◽  
Author(s):  
Daniel Deere ◽  
Jian Shen ◽  
Graham Vesey ◽  
Philip Bell ◽  
Peter Bissinger ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Cell Selection ◽  
Viability Assessment ◽  
Yeast Viability

Brief temperature extremes during wine fermentation: effect on yeast viability and fermentation progress

2018 ◽  
Vol 25 (1) ◽  
pp. 62-69 ◽  
Author(s):  
G.D.S. Valentine ◽  
M.E. Walker ◽  
J.M. Gardner ◽  
F. Schmid ◽  
V. Jiranek
Keyword(s):  
Wine Fermentation ◽  
Temperature Extremes ◽  
Yeast Viability

Comparison of the Methylene Blue Assay with a New Flow-Cytometric Method for Determining Yeast Viability in a Brewery

2008 ◽  
pp. 174-179
Author(s):  
A. Boyd ◽  
T. Gunasekera ◽  
P. Attfield ◽  
K. Simic ◽  
S. Vincent ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Yeast Viability ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals PAC1 Gene Knockout Reveals an Essential Role of Chaperone-Mediated 20S Proteasome Biogenesis and Latent 20S Proteasomes in Cellular Homeostasis

2010 ◽  
Vol 30 (15) ◽  
pp. 3864-3874 ◽  
Author(s):  
Katsuhiro Sasaki ◽  
Jun Hamazaki ◽  
Masato Koike ◽  
Yuko Hirano ◽  
Masaaki Komatsu ◽  
...  
Keyword(s):  
Gene Knockout ◽  
Complex Structure ◽  
Biological Significance ◽  
Conditional Knockout ◽  
26S Proteasome ◽  
20S Proteasome ◽  
Mammalian Development ◽  
Catalytic Core ◽  
Yeast Viability ◽  
Early Embryonic Lethality

ABSTRACT The 26S proteasome, a central enzyme for ubiquitin-dependent proteolysis, is a highly complex structure comprising 33 distinct subunits. Recent studies have revealed multiple dedicated chaperones involved in proteasome assembly both in yeast and in mammals. However, none of these chaperones is essential for yeast viability. PAC1 is a mammalian proteasome assembly chaperone that plays a role in the initial assembly of the 20S proteasome, the catalytic core of the 26S proteasome, but does not cause a complete loss of the 20S proteasome when knocked down. Thus, both chaperone-dependent and -independent assembly pathways exist in cells, but the contribution of the chaperone-dependent pathway remains unclear. To elucidate its biological significance in mammals, we generated PAC1 conditional knockout mice. PAC1-null mice exhibited early embryonic lethality, demonstrating that PAC1 is essential for mammalian development, especially for explosive cell proliferation. In quiescent adult hepatocytes, PAC1 is responsible for producing the majority of the 20S proteasome. PAC1-deficient hepatocytes contained normal amounts of the 26S proteasome, but they completely lost the free latent 20S proteasome. They also accumulated ubiquitinated proteins and exhibited premature senescence. Our results demonstrate the importance of the PAC1-dependent assembly pathway and of the latent 20S proteasomes for maintaining cellular integrity.

scholarly journals Long-Term Adaption to High Osmotic Stress as a Tool for Improving Enological Characteristics in Industrial Wine Yeast

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 576 ◽  
Author(s):  
Gabriela Betlej ◽  
Ewelina Bator ◽  
Bernadetta Oklejewicz ◽  
Leszek Potocki ◽  
Anna Górka ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Grape Juice ◽  
Wine Yeast ◽  
Sensory Profile ◽  
Wine Yeasts ◽  
Adaptive Laboratory Evolution ◽  
Yeast Viability ◽  
Glycerol Synthesis ◽  
The Many

Industrial wine yeasts owe their adaptability in constantly changing environments to a long evolutionary history that combines naturally occurring evolutionary events with human-enforced domestication. Among the many stressors associated with winemaking processes that have potentially detrimental impacts on yeast viability, growth, and fermentation performance are hyperosmolarity, high glucose concentrations at the beginning of fermentation, followed by the depletion of nutrients at the end of this process. Therefore, in this study, we subjected three widely used industrial wine yeasts to adaptive laboratory evolution under potassium chloride (KCl)-induced osmotic stress. At the end of the evolutionary experiment, we evaluated the tolerance to high osmotic stress of the evolved strains. All of the analyzed strains improved their fitness under high osmotic stress without worsening their economic characteristics, such as growth rate and viability. The evolved derivatives of two strains also gained the ability to accumulate glycogen, a readily mobilized storage form of glucose conferring enhanced viability and vitality of cells during prolonged nutrient deprivation. Moreover, laboratory-scale fermentation in grape juice showed that some of the KCl-evolved strains significantly enhanced glycerol synthesis and production of resveratrol-enriched wines, which in turn greatly improved the wine sensory profile. Altogether, these findings showed that long-term adaptations to osmotic stress can be an attractive approach to develop industrial yeasts.

Assessing yeast viability from cell size measurements?

2010 ◽  
Vol 149 (1-2) ◽  
pp. 74-80 ◽  
Author(s):  
Pierre Tibayrenc ◽  
Laurence Preziosi-Belloy ◽  
Jean-Michel Roger ◽  
Charles Ghommidh
Keyword(s):  
Cell Size ◽  
Yeast Viability

scholarly journals Characterization of a Novel Yeast SNARE Protein Implicated in Golgi Retrograde Traffic

1997 ◽  
Vol 8 (12) ◽  
pp. 2659-2676 ◽  
Author(s):  
Vladimir V. Lupashin ◽  
Irina D. Pokrovskaya ◽  
James A. McNew ◽  
M. Gerard Waters
Keyword(s):  
Protein Trafficking ◽  
Sequence Similarity ◽  
Integral Membrane Protein ◽  
Growth Defect ◽  
Carboxypeptidase Y ◽  
Temperature Sensitive ◽  
Snare Protein ◽  
Yeast Viability ◽  
Vacuolar Protein

The protein trafficking machinery of eukaryotic cells is employed for protein secretion and for the localization of resident proteins of the exocytic and endocytic pathways. Protein transit between organelles is mediated by transport vesicles that bear integral membrane proteins (v-SNAREs) which selectively interact with similar proteins on the target membrane (t-SNAREs), resulting in a docked vesicle. A novelSaccharomyces cerevisiae SNARE protein, which has been termed Vti1p, was identified by its sequence similarity to known SNAREs. Vti1p is a predominantly Golgi-localized 25-kDa type II integral membrane protein that is essential for yeast viability. Vti1p can bind Sec17p (yeast SNAP) and enter into a Sec18p (NSF)-sensitive complex with the cis-Golgi t-SNARE Sed5p. This Sed5p/Vti1p complex is distinct from the previously described Sed5p/Sec22p anterograde vesicle docking complex. Depletion of Vti1p in vivo causes a defect in the transport of the vacuolar protein carboxypeptidase Y through the Golgi. Temperature-sensitive mutants of Vti1p show a similar carboxypeptidase Y trafficking defect, but the secretion of invertase and gp400/hsp150 is not significantly affected. The temperature-sensitive vti1 growth defect can be rescued by the overexpression of the v-SNARE, Ykt6p, which physically interacts with Vti1p. We propose that Vti1p, along with Ykt6p and perhaps Sft1p, acts as a retrograde v-SNARE capable of interacting with the cis-Golgi t-SNARE Sed5p.

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