scholarly journals Long-Term Adaption to High Osmotic Stress as a Tool for Improving Enological Characteristics in Industrial Wine Yeast

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 576 ◽  
Author(s):  
Gabriela Betlej ◽  
Ewelina Bator ◽  
Bernadetta Oklejewicz ◽  
Leszek Potocki ◽  
Anna Górka ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Grape Juice ◽  
Wine Yeast ◽  
Sensory Profile ◽  
Wine Yeasts ◽  
Adaptive Laboratory Evolution ◽  
Yeast Viability ◽  
Glycerol Synthesis ◽  
The Many

Industrial wine yeasts owe their adaptability in constantly changing environments to a long evolutionary history that combines naturally occurring evolutionary events with human-enforced domestication. Among the many stressors associated with winemaking processes that have potentially detrimental impacts on yeast viability, growth, and fermentation performance are hyperosmolarity, high glucose concentrations at the beginning of fermentation, followed by the depletion of nutrients at the end of this process. Therefore, in this study, we subjected three widely used industrial wine yeasts to adaptive laboratory evolution under potassium chloride (KCl)-induced osmotic stress. At the end of the evolutionary experiment, we evaluated the tolerance to high osmotic stress of the evolved strains. All of the analyzed strains improved their fitness under high osmotic stress without worsening their economic characteristics, such as growth rate and viability. The evolved derivatives of two strains also gained the ability to accumulate glycogen, a readily mobilized storage form of glucose conferring enhanced viability and vitality of cells during prolonged nutrient deprivation. Moreover, laboratory-scale fermentation in grape juice showed that some of the KCl-evolved strains significantly enhanced glycerol synthesis and production of resveratrol-enriched wines, which in turn greatly improved the wine sensory profile. Altogether, these findings showed that long-term adaptations to osmotic stress can be an attractive approach to develop industrial yeasts.

Preservation of diversity and oenological properties of wine yeasts during long-term laboratory maintenance: A study of strains of a century-old Tokaj wine yeast collection

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 789-798 ◽  
Author(s):  
Zoltán Kállai ◽  
Walter P. Pfliegler ◽  
Judit Mitercsák ◽  
Gergő Szendei ◽  
Matthias Sipiczki
Keyword(s):  
Wine Yeast ◽  
Wine Yeasts ◽  
Laboratory Maintenance

scholarly journals Evaluation of Saccharomyces cerevisiae Wine Yeast Competitive Fitness in Enologically Relevant Environments by Barcode Sequencing

2019 ◽  
Vol 10 (2) ◽  
pp. 591-603 ◽  
Author(s):  
Simon A. Schmidt ◽  
Radka Kolouchova ◽  
Angus H. Forgan ◽  
Anthony R. Borneman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Extraction ◽  
Grape Juice ◽  
Wine Yeast ◽  
Competitive Fitness ◽  
Daunting Task ◽  
The Many ◽  
Grape Juices ◽  
Barcode Sequencing ◽  
Strain Dependent

When a wine yeast is inoculated into grape juice the potential variation in juice composition that confronts it is huge. Assessing the performance characteristics of the many commercially available wine yeasts in the many possible grape juice compositions is a daunting task. To this end we have developed a barcoded Saccharomyces cerevisiae wine yeast collection to facilitate the task of performance assessment that will contribute to a broader understanding of genotype-phenotype relations. Barcode sequencing of mixed populations is used to monitor strain abundance in different grape juices and grape juice-like environments. Choice of DNA extraction method is shown to affect strain-specific barcode count in this highly related set of S. cerevisiae strains; however, the analytical approach is shown to be robust toward strain dependent variation in DNA extraction efficiency. Of the 38 unique compositional variables assessed, resistance to copper and SO2 are found to be dominant discriminatory factors in wine yeast performance. Finally, a comparison of competitive fitness profile with performance in single inoculum fermentations reveal strain dependent correspondence of yeast performance using these two different approaches.

scholarly journals Not all wine yeast are equal

2007 ◽  
Vol 28 (2) ◽  
pp. 55 ◽  
Author(s):  
Eveline Bartowsky ◽  
Jenny Bellon ◽  
Anthony Borneman ◽  
Paul Chambers ◽  
Antonio Cordente ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Concentration ◽  
Grape Juice ◽  
Wine Yeast ◽  
Hostile Environment ◽  
Wine Yeasts ◽  
Final Ethanol Concentration

It may come as a surprise to learn that there are over 200 commercial strains of Saccharomyces cerevisiae available for winemakers to work their magic on grape juice. Why so many? Surely one or two reliable workhorse strains should suffice; after all, don?t they just make ethanol from sugar? The answer to this is an emphatic no; the more we look at the role(s) of yeast in winemaking, the more we are learning about their influences on appearance, aroma, flavour, mouthfeel and final ethanol concentration. And different yeast are more or less robust and efficient in converting the hostile environment of grape juice into wine. Indeed, not all wine yeasts are equal.

The Many Faces of Socioeconomic Change

2017 ◽  
Author(s):  
John Toye
Keyword(s):  
Socioeconomic Development ◽  
Adam Smith ◽  
First Century ◽  
Social Progress ◽  
Long Time ◽  
The Face ◽  
Scientific Expertise ◽  
The Many ◽  
Century Development

This book provides a survey of different ways in which economic sociocultural and political aspects of human progress have been studied since the time of Adam Smith. Inevitably, over such a long time span, it has been necessary to concentrate on highlighting the most significant contributions, rather than attempting an exhaustive treatment. The aim has been to bring into focus an outline of the main long-term changes in the way that socioeconomic development has been envisaged. The argument presented is that the idea of socioeconomic development emerged with the creation of grand evolutionary sequences of social progress that were the products of Enlightenment and mid-Victorian thinkers. By the middle of the twentieth century, when interest in the accelerating development gave the topic a new impetus, its scope narrowed to a set of economically based strategies. After 1960, however, faith in such strategies began to wane, in the face of indifferent results and general faltering of confidence in economists’ boasts of scientific expertise. In the twenty-first century, development research is being pursued using a research method that generates disconnected results. As a result, it seems unlikely that any grand narrative will be created in the future and that neo-liberalism will be the last of this particular kind of socioeconomic theory.

scholarly journals Molecular Basis of Fructose Utilization by the Wine Yeast Saccharomyces cerevisiae: a Mutated HXT3 Allele Enhances Fructose Fermentation

2007 ◽  
Vol 73 (8) ◽  
pp. 2432-2439 ◽  
Author(s):  
Carole Guillaume ◽  
Pierre Delobel ◽  
Jean-Marie Sablayrolles ◽  
Bruno Blondin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Basis ◽  
Alcoholic Fermentation ◽  
Wine Yeast ◽  
Glycolytic Flux ◽  
Hexose Transporter ◽  
Wine Yeasts ◽  
Yeast Saccharomyces Cerevisiae ◽  
High Fructose ◽  
Fructose Fermentation

ABSTRACT Fructose utilization by wine yeasts is critically important for the maintenance of a high fermentation rate at the end of alcoholic fermentation. A Saccharomyces cerevisiae wine yeast able to ferment grape must sugars to dryness was found to have a high fructose utilization capacity. We investigated the molecular basis of this enhanced fructose utilization capacity by studying the properties of several hexose transporter (HXT) genes. We found that this wine yeast harbored a mutated HXT3 allele. A functional analysis of this mutated allele was performed by examining expression in an hxt1-7Δ strain. Expression of the mutated allele alone was found to be sufficient for producing an increase in fructose utilization during fermentation similar to that observed in the commercial wine yeast. This work provides the first demonstration that the pattern of fructose utilization during wine fermentation can be altered by expression of a mutated hexose transporter in a wine yeast. We also found that the glycolytic flux could be increased by overexpression of the mutant transporter gene, with no effect on fructose utilization. Our data demonstrate that the Hxt3 hexose transporter plays a key role in determining the glucose/fructose utilization ratio during fermentation.

scholarly journals LifePlanning: A Better Retirement Planning Option for Our Times

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 111-112
Author(s):  
Rajiv Nagaich ◽  
Carol Redfield ◽  
Ben Harvill
Keyword(s):  
Institutional Care ◽  
Integrated Approach ◽  
Retirement Planning ◽  
Practice Research ◽  
Care Needs ◽  
Term Care ◽  
Investment Returns ◽  
The Many ◽  
In The Beginning

Abstract Ten thousand turn 65 daily. Majority look forward to retiring in the beginning and then become afraid of outcomes they often hear about- dealing with institutional care, becoming a burden, or running out of money. This is not because retirees do not plan, but despite of having planned their entire life for retirement. Many employers provide financial retirement planning such as a 401K plan. Individuals have relied on employee benefit plans to ready themselves, yet few are “very confident” about it. Two-thirds of retirees say their most recent employers did “nothing” to help them transition into retirement; 16% are “not sure” what their employers did. Many may be overlooking important factors in their strategies. Among retirees who currently have a retirement strategy, 85% have factored Social Security and Medicare benefits into their strategy. Most have included on-going living expenses (79%), total savings and income needs (57%) into their plan. Fewer than half have considered other critical factors (e.g., investment returns, ongoing healthcare costs, inflation, long-term care needs, tax planning, etc.). Only 9% have contingency plans for retiring sooner than expected and/or savings shortfalls. The truth is that education offered by employers tends to be traditional planning advice, which may not be enough to address the concerns retirees will have in retirement. To this, we introduce a multi-disciplinary LifePlanning Framework which takes a wholistic, integrated approach in addressing the many complex issues of retirement found in health, housing, finance, legal, and family. Our results may impact future practice, research, and policy.

scholarly journals Radiation Necrosis of the Lateral Skull Base and Temporal Bone

2020 ◽  
Vol 34 (04) ◽  
pp. 265-271
Author(s):  
Marc W. Herr ◽  
Aurora G. Vincent ◽  
Meghan A. Skotnicki ◽  
Yadranko Ducic ◽  
Spiros Manolidis
Keyword(s):  
Radiation Therapy ◽  
Temporal Bone ◽  
Radiation Necrosis ◽  
Critical Role ◽  
Therapeutic Effects ◽  
Normal Tissues ◽  
Lateral Skull Base ◽  
The Many ◽  
Work Up

AbstractRadiation therapy plays a critical role in the treatment of malignancies involving the head and neck. Although the therapeutic effects of ionizing radiation are achieved, normal tissues are also susceptible to injury and significant long-term sequelae. Osteoradionecrosis of the temporal bone (ORNTB) is among the many complications that can arise after therapy. ORNTB is a debilitating and potentially lethal condition that continues to challenge patients and treating physicians. Herein, we review the pathophysiology, presentation, work-up, and management of ORNTB.

scholarly journals Learning about Stellar Dynamos from Long–term Photometry of Starspots

1991 ◽  
Vol 130 ◽  
pp. 353-369 ◽  
Author(s):  
Douglas S. Hall
Keyword(s):  
Differential Rotation ◽  
Rotation Period ◽  
Turnover Time ◽  
Rotating Stars ◽  
Photometric Variability ◽  
Solar Type ◽  
Magnetic Cycles ◽  
The Many ◽  
Rapidly Rotating Stars

AbstractSpottedness, as evidenced by photometric variability in 277 late-type binary and single stars, is found to occur when the Rossby number is less than about 2/3. This holds true when the convective turnover time versus B–V relation of Gilliland is used for dwarfs and also for subgiants and giants if their turnover times are twice and four times longer, respectively, than for dwarfs. Differential rotation is found correlated with rotation period (rapidly rotating stars approaching solid-body rotation) and also with lobe-filling factor (the differential rotation coefficient k is 2.5 times larger for F = 0 than F = 1). Also reviewed are latitude extent of spottedness, latitude drift during a solar-type cycle, sector structure and preferential longitudes, starspot lifetimes, and the many observational manifestations of magnetic cycles.

scholarly journals Reframing Aging in Contemporary Politics: Building Momentum

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 681-681
Author(s):  
Patricia D'Antonio
Keyword(s):  
Social Change ◽  
Older People ◽  
Evidence Based ◽  
Negative Attitudes ◽  
Attitudes And Behaviors ◽  
Contemporary Politics ◽  
Perceptions Of Aging ◽  
The Many ◽  
Changing Attitudes

Abstract Changing American culture is challenging and changing attitudes and behaviors around the universal experience of aging especially so. Unless the field of advocates who care about aging issues cultivates a more visible, more informed conversation on older people, it will remain difficult to advance the systemic changes needed to adjust to a society with increased and increasing longevity. Advocates will need to be vigilant to avoid cueing negative attitudes towards aging and aging policies. The Reframing Aging Initiative is a long-term, social change endeavor designed to improve the public’s understanding of what aging means and the many contributions older people bring to society. Using evidence-based research, the initiative seeks to teach advocates how to tell an effective story about aging that will promote positive perceptions of aging and reduce ageism. The time to change the conversation is now.

scholarly journals The Fitness Advantage of Commercial Wine Yeasts in Relation to the Nitrogen Concentration, Temperature, and Ethanol Content under Microvinification Conditions

2013 ◽  
Vol 80 (2) ◽  
pp. 704-713 ◽  
Author(s):  
Estéfani García-Ríos ◽  
Alicia Gutiérrez ◽  
Zoel Salvadó ◽  
Francisco Noé Arroyo-López ◽  
José Manuel Guillamon
Keyword(s):  
Abiotic Factors ◽  
Nitrogen Concentration ◽  
Wine Yeast ◽  
Wine Industry ◽  
Wine Yeasts ◽  
Simple Method ◽  
Fitness Advantage ◽  
Yeast Strains ◽  
Industrial Strains ◽  
Nitrogen Concentrations

ABSTRACTThe effect of the main environmental factors governing wine fermentation on the fitness of industrial yeast strains has barely received attention. In this study, we used the concept of fitness advantage to measure how increasing nitrogen concentrations (0 to 200 mg N/liter), ethanol (0 to 20%), and temperature (4 to 45°C) affects competition among four commercial wine yeast strains (PDM, ARM, RVA, and TTA). We used a mathematical approach to model the hypothetical time needed for the control strain (PDM) to out-compete the other three strains in a theoretical mixed population. The theoretical values obtained were subsequently verified by competitive mixed fermentations in both synthetic and natural musts, which showed a good fit between the theoretical and experimental data. Specifically, the data show that the increase in nitrogen concentration and temperature values improved the fitness advantage of the PDM strain, whereas the presence of ethanol significantly reduced its competitiveness. However, the RVA strain proved to be the most competitive yeast for the three enological parameters assayed. The study of the fitness of these industrial strains is of paramount interest for the wine industry, which uses them as starters of their fermentations. Here, we propose a very simple method to model the fitness advantage, which allows the prediction of the competitiveness of one strain with respect to different abiotic factors.

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