Biochemical Detection of cGMP From Past to Present: An Overview

2009 ◽  
pp. 195-228 ◽  
Author(s):  
Peter M. Schmidt
Keyword(s):  
Biochemical Detection

scholarly journals Biochemical detection of capsid in the nucleus during HIV-1 infection

2021 ◽  
Vol 2 (1) ◽  
pp. 100323
Author(s):  
Anastasia Selyutina ◽  
Felipe Diaz-Griffero
Keyword(s):  
Biochemical Detection ◽  
Hiv 1

Biochemical detection of systolic dysfunction

The Lancet ◽  
1998 ◽  
Vol 351 (9108) ◽  
pp. 1063-1064
Author(s):  
T McDonagh ◽  
A Harkness ◽  
HJ Dargie
Keyword(s):  
Systolic Dysfunction ◽  
Biochemical Detection

Biochemical detection of left-ventricular systolic dysfunction

The Lancet ◽  
1998 ◽  
Vol 351 (9095) ◽  
pp. 9-13 ◽  
Author(s):  
TA McDonagh ◽  
SD Robb ◽  
DR Murdoch ◽  
JJ Morton ◽  
I Ford ◽  
...  
Keyword(s):  
Left Ventricular Systolic Dysfunction ◽  
Systolic Dysfunction ◽  
Left Ventricular ◽  
Ventricular Systolic Dysfunction ◽  
Biochemical Detection

Immunohistochemical and Biochemical Detection of Low Density Lipoprotein Receptors in Cultured Rat Mesangial Cells

Nephron ◽  
1995 ◽  
Vol 69 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Hiroo Ito ◽  
Minoru Chimata ◽  
Megumi Oka ◽  
Yasuyuki Suzuki ◽  
Natsuko Hamada ◽  
...  
Keyword(s):  
Mesangial Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptors ◽  
Biochemical Detection

scholarly journals Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions

Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1380
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Changcheng Qian ◽  
A. S. M. Muhtasim Fuad Sohan ◽  
Teng Zhou ◽  
...  
Keyword(s):  
Metal Ions ◽  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Copper Ions ◽  
Practical Applications ◽  
Enzyme Method ◽  
In Series ◽  
High Concentrations ◽  
Biochemical Detection ◽  
Seawater Samples

Metal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu2+ contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu2+ in seawater. In this method, Cu2+ is reduced to Cu+ to inhibit horseradish peroxidase (HRP) activity, which then results in the color distortion of the reaction solution. The chip provides both naked eye and spectrophotometer modalities. Cu2+ concentrations have an ideal linear relationship, with absorbance values ranging from 3.91 nM to 256 μM. The proposed enzyme method-based microfluidic chip detects Cu2+ with a limit of detection (LOD) of 0.87 nM. Other common metal ions do not affect the operation of the chip. The successful detection of Cu2+ was achieved using three real seawater samples, verifying the ability of the chip in practical applications. Furthermore, the chip realizes the functions of two AND gates in series and has potential practical implementations in biochemical detection and biological computing.

scholarly journals Biochemical detection of E-ADA on Neospora caninum tachyzoites and the effects of a specific enzymatic inhibitor

2015 ◽  
pp. 4455-4460
Author(s):  
Alexandre A. Tonin ◽  
Aleksandro S. Da Silva ◽  
Victor C. Pimentel ◽  
Daniele Zanini ◽  
Maria Rosa C. Schetinger ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Activity ◽  
Adenosine Deaminase ◽  
Protein Concentration ◽  
Negative Impact ◽  
Neospora Caninum ◽  
Free Form ◽  
Biochemical Detection ◽  
Caninum Tachyzoites ◽  
Enzymatic Inhibitor

Objective. This study aimed to investigate the presence and activity of the ecto adenosine deaminase (E-ADA) enzyme in tachyzoites of Neospora caninum (Nc-1 strain), as well as to assess the activity of a well-known E-ADA inhibitor, the deoxycoformycin. Materials and methods. The parasites were grown in cell culture, being subsequently separated in a pellet of tachyzoites, on which the E-ADA activity was tested using the concentrations 0 (control), 0.2, 0.4 and 0.8 mg mL-1. Results. The E-ADA showed high activity, progressively increasing its activity according to the enhancement of the protein concentration. The test was carried out with different concentrations of deoxycoformycin, showing that it was able to inhibit the E-ADA present on the free form of the parasite. Conclusions. Based on these results we conclude that the E-ADA is present on tachyzoites of N. caninum, and deoxycoformycin is able to inhibit this enzyme. In this sense, knowing the negative impact of N. caninum on reproductive issue in cattle (mainly abortion), might it is an alternative in order to deal with this parasitic infection.Key words: adenosine deaminase, deoxycoformycin, neosporosis (Source: CAB, MeSH).

Development and Characterization of a Generic Microfluidic Subsystem toward Portable Biochemical Detection

2000 ◽  
pp. 327-330 ◽  
Author(s):  
Jin-Woo Choi ◽  
C. Ajith Wijayawardhana ◽  
Nihat Okulan ◽  
Kwang W. Oh ◽  
Arum Han ◽  
...  
Keyword(s):  
Biochemical Detection

A Polymer-Based Microfluidic Sensor for Biochemical Detection

2020 ◽  
Vol 20 (12) ◽  
pp. 6270-6276
Author(s):  
Jianze Huang ◽  
Yang Liu ◽  
Zhiyong Tang ◽  
Xiufeng Shao ◽  
Congchun Zhang
Keyword(s):  
Biochemical Detection

Cystic fibrosis--its biochemical detection.

1983 ◽  
Vol 29 (12) ◽  
pp. 2011-2018 ◽  
Author(s):  
A F Heeley ◽  
D Watson
Keyword(s):  
Cystic Fibrosis ◽  
Filter Paper ◽  
Neonatal Screening ◽  
Duodenal Juice ◽  
Heterozygous State ◽  
Intestinal Malabsorption ◽  
Pancreatic Lesion ◽  
Pancreatic Dysfunction ◽  
Pancreatic Isoamylase ◽  
Biochemical Detection

Abstract We examine critically the biochemical methods capable of detecting and monitoring the end-organ disease processes in patients with cystic fibrosis. Although the diagnosis of cystic fibrosis is never justified on the basis of the sweat salt test alone, the original filter-paper technic (Gibson-Cooke, Pediatrics 23:545-549, 1959) for determining Na+ and Cl- concentrations in sweat remains the most discriminating method. We discuss the contributions for neonatal screening of the so-called cystic fibrosis protein, associated decreased enzymic activities in the homo- and heterozygous state, and immunoreactive trypsin. Because evidence of either intestinal malabsorption or a pancreatic lesion must be sought, we review the use and interpretation of some tests of pancreatic dysfunction (meconium albumin, duodenal juice components, serum pancreatic isoamylase, and trypsinogen), both in establishing and in confirming the diagnosis of cystic fibrosis.

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