Specialized Components of the Translational Machinery for Unnatural Amino Acid Mutagenesis: tRNAs, Aminoacyl-tRNA Synthetases, and Ribosomes

2009 ◽  
pp. 205-229 ◽  
Author(s):  
C. Köhrer ◽  
U. L. RajBhandary
Keyword(s):  
Amino Acid ◽  
Unnatural Amino Acid ◽  
Translational Machinery ◽  
Trna Synthetases ◽  
Unnatural Amino Acid Mutagenesis ◽  
Aminoacyl Trna Synthetases ◽  
Amino Acid Mutagenesis

Unnatural amino acid mutagenesis in mapping ion channel function

2003 ◽  
Vol 13 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Darren L Beene ◽  
Dennis A Dougherty ◽  
Henry A Lester
Keyword(s):  
Amino Acid ◽  
Ion Channel ◽  
Unnatural Amino Acid ◽  
Channel Function ◽  
Unnatural Amino Acid Mutagenesis ◽  
Amino Acid Mutagenesis

scholarly journals Gating modules of the AMPA receptor pore domain revealed by unnatural amino acid mutagenesis

2019 ◽  
Vol 116 (27) ◽  
pp. 13358-13367 ◽  
Author(s):  
Mette H. Poulsen ◽  
Anahita Poshtiban ◽  
Viktoria Klippenstein ◽  
Valentina Ghisi ◽  
Andrew J. R. Plested
Keyword(s):  
Amino Acid ◽  
Conformational Changes ◽  
Transmembrane Domain ◽  
Uv Light ◽  
Unnatural Amino Acid ◽  
Selectivity Filter ◽  
Unnatural Amino Acid Mutagenesis ◽  
Amino Acid Mutagenesis ◽  
Mutant Receptors ◽  
Pore Domain

Ionotropic glutamate receptors (iGluRs) are responsible for fast synaptic transmission throughout the vertebrate nervous system. Conformational changes of the transmembrane domain (TMD) underlying ion channel activation and desensitization remain poorly understood. Here, we explored the dynamics of the TMD of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type iGluRs using genetically encoded unnatural amino acid (UAA) photocross-linkers, p-benzoyl-l-phenylalanine (BzF) and p-azido-l-phenylalanine (AzF). We introduced these UAAs at sites throughout the TMD of the GluA2 receptor and characterized the mutants in patch-clamp recordings, exposing them to glutamate and ultraviolet (UV) light. This approach revealed a range of optical effects on the activity of mutant receptors. We found evidence for an interaction between the Pre-M1 and the M4 TMD helix during desensitization. Photoactivation at F579AzF, a residue behind the selectivity filter in the M2 segment, had extraordinarily broad effects on gating and desensitization. This observation suggests coupling to other parts of the receptor and like in other tetrameric ion channels, selectivity filter gating.

Analysis of the Role of the Active Site Tyrosine in Human Glutathione Transferase A1-1 by Unnatural Amino Acid Mutagenesis

1998 ◽  
Vol 120 (2) ◽  
pp. 451-452 ◽  
Author(s):  
Jon S. Thorson ◽  
Injae Shin ◽  
Eli Chapman ◽  
Gun Stenberg ◽  
Bengt Mannervik ◽  
...  
Keyword(s):  
Amino Acid ◽  
Active Site ◽  
Glutathione Transferase ◽  
Unnatural Amino Acid ◽  
Unnatural Amino Acid Mutagenesis ◽  
Active Site Tyrosine ◽  
Amino Acid Mutagenesis

scholarly journals Investigation of Trimethyllysine Binding by the HP1 Chromodomain via Unnatural Amino Acid Mutagenesis

2017 ◽  
Vol 139 (48) ◽  
pp. 17253-17256 ◽  
Author(s):  
Stefanie A. Baril ◽  
Amber L. Koenig ◽  
Mackenzie W. Krone ◽  
Katherine I. Albanese ◽  
Cyndi Qixin He ◽  
...  
Keyword(s):  
Amino Acid ◽  
Unnatural Amino Acid ◽  
Unnatural Amino Acid Mutagenesis ◽  
Amino Acid Mutagenesis

scholarly journals A Novel Method to Probe Membrane Protein Topology Using Unnatural Amino Acid Mutagenesis and Antibody Epitope Tagging

2010 ◽  
Vol 98 (3) ◽  
pp. 419a
Author(s):  
Saranga Naganathan ◽  
Shixin Ye ◽  
Terence Duarte ◽  
Thomas Huber ◽  
Pallavi Sachdev ◽  
...  
Keyword(s):  
Amino Acid ◽  
Membrane Protein ◽  
Unnatural Amino Acid ◽  
Epitope Tagging ◽  
Protein Topology ◽  
Unnatural Amino Acid Mutagenesis ◽  
Membrane Protein Topology ◽  
Antibody Epitope ◽  
Novel Method ◽  
Amino Acid Mutagenesis

scholarly journals Testing Models for the Slow Inactivated State using Unnatural Amino Acid Mutagenesis

2012 ◽  
Vol 102 (3) ◽  
pp. 530a
Author(s):  
Francis Valiyaveetil ◽  
Alexander Komarov ◽  
Prasanna Devaraneni
Keyword(s):  
Amino Acid ◽  
Unnatural Amino Acid ◽  
Unnatural Amino Acid Mutagenesis ◽  
Amino Acid Mutagenesis
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