How Strong is the Beating of Cardiac Myocytes? – The Cell Drum Solution

2008 ◽  
pp. 351-369
Author(s):  
Jürgen Trzewik ◽  
Peter Linder ◽  
Kay F. Zerlin
Keyword(s):  
Cardiac Myocytes

Cardiac myocytes cultured on a basement membrane extract of the ehs tumor

1986 ◽  
Vol 44 ◽  
pp. 270-271
Author(s):  
L. Terracio ◽  
A. Dewey ◽  
K. Rubin ◽  
T.K. Borg
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cardiac Myocytes ◽  
Normal Tissue ◽  
Cell Spreading ◽  
Collagen Iv ◽  
Basement Membrane Extract ◽  
Membrane Extract ◽  
Growth Development ◽  
Multicellular Organisms

The recognition and interaction of cells with the extracellular matrix (ECM) effects the normal physiology as well as the pathology of all multicellular organisms. These interactions have been shown to influence the growth, development, and maintenance of normal tissue function. In previous studies, we have shown that neonatal cardiac myocytes specifically interacts with a variety of ECM components including fibronectin, laminin, and collagens I, III and IV. Culturing neonatal myocytes on laminin and collagen IV induces an increased rate of both cell spreading and sarcomerogenesis.

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

Small Heat Shock Proteins and Protection Against Ischemic Injury in Cardiac Myocytes

Circulation ◽  
1997 ◽  
Vol 96 (12) ◽  
pp. 4343-4348 ◽  
Author(s):  
Jody L. Martin ◽  
Ruben Mestril ◽  
Randa Hilal-Dandan ◽  
Laurence L. Brunton ◽  
Wolfgang H. Dillmann
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Cardiac Myocytes ◽  
Ischemic Injury ◽  
Small Heat Shock Proteins ◽  
Shock Proteins

Lactate and P o 2 Modulate Superoxide Anion Production in Bovine Cardiac Myocytes

Circulation ◽  
1997 ◽  
Vol 96 (2) ◽  
pp. 614-620 ◽  
Author(s):  
Kamal M. Mohazzab-H. ◽  
Pawel M. Kaminski ◽  
Michael S. Wolin
Keyword(s):  
Cardiac Myocytes ◽  
Superoxide Anion ◽  
Superoxide Anion Production ◽  
Anion Production
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