Molecular Imaging: Reporter Gene Imaging

2008 ◽  
pp. 167-223 ◽  
Author(s):  
Inna Serganova ◽  
Phillipp Mayer-Kukuck ◽  
Ruimin Huang ◽  
Ronald Blasberg
Keyword(s):  
Molecular Imaging ◽  
Reporter Gene ◽  
Reporter Gene Imaging

scholarly journals Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ho Won Lee ◽  
Seung Yun Yoon ◽  
Thoudam Debraj Singh ◽  
Yoon Ju Choi ◽  
Hong Je Lee ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Molecular Imaging ◽  
Lymph Nodes ◽  
Injection Site ◽  
Reporter Gene ◽  
Sodium Iodide ◽  
Firefly Luciferase ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging ◽  
Pet Ct

Abstract We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.

scholarly journals In vivo Monitoring of microRNA Biogenesis Using Reporter Gene Imaging

Theranostics ◽  
2013 ◽  
Vol 3 (12) ◽  
pp. 1004-1011 ◽  
Author(s):  
So Won Oh ◽  
Do Won Hwang ◽  
Dong Soo Lee
Keyword(s):  
Reporter Gene ◽  
Microrna Biogenesis ◽  
Reporter Gene Imaging ◽  
In Vivo Monitoring

Reporter Gene Imaging with PET/SPECT

2010 ◽  
pp. 70-87 ◽  
Author(s):  
June-Key Chung ◽  
Joo Hyun Kang ◽  
Keon Wook Kang
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Imaging

scholarly journals PET Reporter Gene Imaging and Ganciclovir-Mediated Ablation of Chimeric Antigen Receptor T Cells in Solid Tumors

2020 ◽  
Vol 80 (21) ◽  
pp. 4731-4740 ◽  
Author(s):  
Surya Murty ◽  
Louai Labanieh ◽  
Tara Murty ◽  
Gayatri Gowrishankar ◽  
Tom Haywood ◽  
...  
Keyword(s):  
T Cells ◽  
Solid Tumors ◽  
Reporter Gene ◽  
Chimeric Antigen Receptor ◽  
Antigen Receptor ◽  
Reporter Gene Imaging

Lifelong reporter gene imaging in the lungs of mice following polyethyleneimine-mediated sleeping-beauty transposon delivery

Biomaterials ◽  
2011 ◽  
Vol 32 (7) ◽  
pp. 1978-1985 ◽  
Author(s):  
Erh-Hsuan Lin ◽  
Michelle Keramidas ◽  
Claire Rome ◽  
Wen-Ta Chiu ◽  
Cheng-Wen Wu ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Sleeping Beauty ◽  
Reporter Gene Imaging ◽  
Sleeping Beauty Transposon
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