Dual Reporter Gene Imaging for Tracking Macrophage Migration Using the Human Sodium Iodide Symporter and an Enhanced Firefly Luciferase in a Murine Inflammation Model

2013 ◽  
Vol 15 (6) ◽  
pp. 703-712 ◽  
Author(s):  
Ho Won Lee ◽  
Yong Hyun Jeon ◽  
Mi-Hye Hwang ◽  
Jung-Eun Kim ◽  
Tae-in Park ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Sodium Iodide ◽  
Firefly Luciferase ◽  
Macrophage Migration ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging ◽  
Inflammation Model ◽  
Dual Reporter

scholarly journals Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ho Won Lee ◽  
Seung Yun Yoon ◽  
Thoudam Debraj Singh ◽  
Yoon Ju Choi ◽  
Hong Je Lee ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Molecular Imaging ◽  
Lymph Nodes ◽  
Injection Site ◽  
Reporter Gene ◽  
Sodium Iodide ◽  
Firefly Luciferase ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging ◽  
Pet Ct

Abstract We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.

Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium–iodide symporter

2007 ◽  
Vol 2 (5) ◽  
pp. 240-247 ◽  
Author(s):  
Stephen L. Brown ◽  
Svend O. Freytag ◽  
Kenneth N. Barton ◽  
Michael J. Flynn ◽  
Donald J. Peck ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging ◽  
Radiographic Contrast

Design Considerations for Incorporating Sodium Iodide Symporter Reporter Gene Imaging into Prostate Cancer Gene Therapy Trials

2007 ◽  
Vol 18 (4) ◽  
pp. 312-322 ◽  
Author(s):  
Farzan Siddiqui ◽  
Kenneth N. Barton ◽  
Hans J. Stricker ◽  
Phillip F. Steyn ◽  
Susan M. LaRue ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Reporter Gene ◽  
Sodium Iodide ◽  
Cancer Gene Therapy ◽  
Cancer Gene ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging ◽  
Design Considerations

scholarly journals Cardiac reporter gene imaging using the human sodium/iodide symporter gene

2005 ◽  
Vol 65 (1) ◽  
pp. 195-202 ◽  
Author(s):  
M MIYAGAWA ◽  
M BEYER ◽  
B WAGNER ◽  
M ANTON ◽  
C SPITZWEG ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging

Liver Detargeting of Adenoviral Vectors by Polymer Coating after Systemic Delivery Using the Sodium Iodide Symporter (NIS) as Reporter Gene

2011 ◽  
pp. P2-678-P2-678
Author(s):  
Geoffrey Ken Grunwald ◽  
Alexandra Vetter ◽  
Kathrin Klutz ◽  
Nathalie Wunderlich ◽  
Reingard Senekowitsch-Schmidtke ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Polymer Coating ◽  
Sodium Iodide ◽  
Adenoviral Vectors ◽  
Sodium Iodide Symporter ◽  
Systemic Delivery
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