Effectiveness of Phosphate Solubilizing Microorganism in Increasing Plant Phosphate Uptake and Growth in Tropical Soils

The objectives of the research were: (i) to isolate and characterize of phosphate solubilizing bacteria (PSB) and (ii) to identify PSB based on molecular amplification of 16S rRNA gene. Soil samples were collected from rhizosphere in Bogor, West Nusa Tenggara, and East Nusa Tenggara. Several stages in this research were: (i) isolation PSB in Pikovskaya agar, (ii) morphological and biochemical characterization of PSB, (iii) measurement of phosphatase enzymes, and (iv) measurement of secreting indole acetic acid phytohormone. As many as 29 isolates of PSB have been collected and three isolates of them, namely: P 3.5 (East Nusa Tenggara), P 6.2 (West Nusa Tenggara), and P 10.1 (Citeureup, West Java) were chosen for further study. There were many characteristics of isolate P 10.1: (i) it had capable to solubilize P with the value of highest solubilization index (1.80), (ii) it had the highest phosphatase enzyme (120.40 mg kg-1), and (iii) it had the highest pH decrease at each observation for six days. Isolates P 3.5 and P 10.1 were the Gram-negative bacteria with coccus shapes and isolate P 6.2 was a Gram-negative bacteria with bacillus shape. Deoxiribonucleat Acid (DNA) amplification of these bacteria employing 16S rRNA primers generated the 1,300bp-PCR product. The results of the analysis of 16S rRNA gene sequences showed that isolates P 3.5 and P 10.1 has 98% similarity with Gluconacetobacter sp. strains Rg1-MS-CO and isolate P 6.2 has 97% similarity with Enterobacter sp. pp9c strains.Keywords: 16S rRNA, indole acetic acid, isolation, phosphatase enzymes, phosphate solubilizing bacteria[How to Cite : Hazra F and E Pratiwi. 2013. Isolation, Characterization, and Molecular Identification of Phosphate Solubilizing Bacteria from Several Tropical Soils. J Trop Soils, 18 (1): 67-74. doi: 10.5400/jts.2013.18.1.67][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.67]

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Straw biochar increases the abundance of inorganic phosphate solubilizing bacterial community for better rape (Brassica napus) growth and phosphate uptake

The Science of The Total Environment ◽

10.1016/j.scitotenv.2018.07.454 ◽

2019 ◽

Vol 647 ◽

pp. 1113-1120 ◽

Cited By ~ 19

Author(s):

Bang-Xiao Zheng ◽

Kai Ding ◽

Xiao-Ru Yang ◽

Mohammed A.M. Wadaan ◽

Wael N. Hozzein ◽

...

Keyword(s):

Brassica Napus ◽

Bacterial Community ◽

Inorganic Phosphate ◽

Phosphate Uptake ◽

Phosphate Solubilizing

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Co-inoculation with an arbuscular mycorrhizal fungus and a phosphate-solubilizing fungus promotes the plant growth and phosphate uptake of avocado plantlets in a nursery

Botany ◽

10.1139/cjb-2016-0224 ◽

2017 ◽

Vol 95 (5) ◽

pp. 539-545 ◽

Cited By ~ 10

Author(s):

Alvaro Tamayo-Velez ◽

Nelson Walter Osorio

Keyword(s):

Plant Growth ◽

Phosphate Uptake ◽

Arbuscular Mycorrhizal Fungus ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Dry Mass ◽

Persea Americana ◽

Plant Performance ◽

Uninoculated Control ◽

Phosphate Solubilizing

An experiment was conducted to evaluate the individual and combined effects of treatment with the phosphate solubilizing fungus Mortierella sp. and the arbuscular mycorrhizal fungus Rhizoglomus fasciculatum on the plant growth and phosphate uptake on plantlets of avocado (Persea americana Mill. cv. ‘Hass’) grown in a nursery. A completely randomized test design was used. Treatments consisted of individual and combined inoculations with R. fasciculatum and Mortierella sp. at two concentrations (106and 108CFU·mL−1), and the results were compared with an uninoculated control. The plant height, shoot dry mass, and shoot phosphate uptake were significantly higher in plants inoculated with both of the fungi than with either fungus individually, or in the uninoculated control plants. The colonization of fine roots with both fungi decreased when they were co-inoculated by comparison with when they were individually inoculated, which suggests that these fungi compete for root space. Despite this competition, the dual inoculation showed that the fungi had additive effects on plant performance. Thus, shoot phosphate levels in plantlets inoculated with mycorrhizae was significantly higher when Mortierella sp. was co-inoculated at both concentrations, compared with the single inoculations and the uninoculated control plants (mycorrhiza free).

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