Endogenous Control of DeGroot Learning

Author(s):  
Sridhar Mandyam ◽  
Usha Sridhar
Keyword(s):  
2004 ◽  
Vol 67 (6) ◽  
pp. 1141-1149 ◽  
Author(s):  
John D Styrsky ◽  
Peter Berthold ◽  
W.Douglas Robinson

2011 ◽  
Vol 40 (1) ◽  
pp. 124-128
Author(s):  
Sabine Wohlres-Viana ◽  
Mariana Cortes Boite ◽  
João Henrique Moreira Viana ◽  
Marco Antonio Machado ◽  
Luiz Sérgio de Almeida Camargo

The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase α1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase β2 transcripts 0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase β2 genes, however, it affects expression of Na/K-ATPase α1.


2005 ◽  
Vol 95 (6) ◽  
pp. 891-900 ◽  
Author(s):  
ACHIM WALTER ◽  
ULRICH SCHURR

2010 ◽  
Vol 12 (6) ◽  
pp. 570-579 ◽  
Author(s):  
S. Kreth ◽  
J. Heyn ◽  
S. Grau ◽  
H. A. Kretzschmar ◽  
R. Egensperger ◽  
...  

2000 ◽  
pp. 114-130 ◽  
Author(s):  
Alexander Shnyra ◽  
Michael Luchi ◽  
Jiangjun Gao ◽  
Christopher J. Papasian ◽  
David L. Horn ◽  
...  

2006 ◽  
Vol 6 ◽  
pp. 1048-1065 ◽  
Author(s):  
Thea Morris ◽  
Melanie Stables ◽  
Derek W. Gilroy

Aspirin is unique among the nonsteroidal anti-inflammatory drugs in that it has both anti-inflammatory as well as cardio-protective properties. The cardio-protective properties arise form its judicious inhibition of platelet-derived thromboxane A2over prostacyclin, while its anti-inflammatory effects of aspirin stem from its well-established inhibition of prostaglandin (PG) synthesis within inflamed tissues. Thus aspirin and the other NSAIDs have popularised the notion of inhibiting PG biosynthesis as a common anti-inflammatory strategy based on the erroneous premise that all eicosanoids are generally detrimental to inflammation. However, our fascination with aspirin has shown a more affable side to lipid mediators based on our increasing interest in the endogenous control of acute inflammation and in factors that mediate its resolution. Epi-lipoxins (epi-LXs), for instance, are produced from aspirin’s acetylation of inducible cyclooxygenase 2 (COX-2) and together with Resolvins represent an increasingly important family of immuno-regulatory and potentially cardio-protective lipid mediators. Aspirin is beginning to teach us what nature knew all along – that not all lipid mediators are bad. It seems that while some eicosanoids are pathogenic in a variety of diseases, others are unarguable protective. In this review we will re-count aspirin’s colorful history, discuss its traditional mode of action and the controversies associated therewith, as well as highlight some of the new pathways in inflammation and the cardiovascular systems that aspirin has recently revealed.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tim A. D. Smith ◽  
Omneya A. AbdelKarem ◽  
Joely J. Irlam-Jones ◽  
Brian Lane ◽  
Helen Valentine ◽  
...  

Abstract Quantitative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes. We aimed to: (1) demonstrate a pathway to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE) tissue using bladder cancer as an exemplar; and (2) examine the influence of probe length and sample age on PCR amplification and co-expression of candidate genes on apparent expression stability. RNA was extracted from prospective and retrospective samples and subject to qPCR using TaqMan human endogenous control arrays or single tube assays. Gene stability ranking was assessed using coefficient of variation (CoV), GeNorm and NormFinder. Co-expressed genes were identified from The Cancer Genome Atlas (TCGA) using the on-line gene regression analysis tool GRACE. Cycle threshold (Ct) values were lower for prospective (19.49 ± 2.53) vs retrospective (23.8 ± 3.32) tissues (p < 0.001) and shorter vs longer probes. Co-expressed genes ranked as the most stable genes in the TCGA cohort by GeNorm when analysed together but ranked lower when analysed individually omitting co-expressed genes indicating bias. Stability values were < 1.5 for the 20 candidate genes in the prospective cohort. As they consistently ranked in the top ten by CoV, GeNorm and Normfinder, UBC, RPLP0, HMBS, GUSB, and TBP are the most suitable endogenous control genes for bladder cancer qPCR.


Stem Cells ◽  
2010 ◽  
pp. N/A-N/A
Author(s):  
Kasper Grit ◽  
Ode Andrea ◽  
Groothuis Aline ◽  
Glaeser Juliane ◽  
Gaber Timo ◽  
...  

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