Nuclear Stability

Author(s):  
Alain Coc
Keyword(s):  
1995 ◽  
Vol 10 (5) ◽  
pp. 1280-1286 ◽  
Author(s):  
Jorge Molina ◽  
Jose Antonio Castilla ◽  
Teresa Gil ◽  
Maria Luisa Hortas ◽  
Francisco Vergara ◽  
...  

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Yulia Vasianovich ◽  
Emmanuel Bajon ◽  
Raymund J Wellinger

The templating RNA is the core of the telomerase reverse transcriptase. In Saccharomyces cerevisiae, the complex life cycle and maturation of telomerase includes a cytoplasmic stage. However, timing and reason for this cytoplasmic passage are poorly understood. Here, we use inducible RNA tagging experiments to show that immediately after transcription, newly synthesized telomerase RNAs undergo one round of nucleo-cytoplasmic shuttling. Their export depends entirely on Crm1/Xpo1, whereas re-import is mediated by Kap122 plus redundant, kinetically less efficient import pathways. Strikingly, Mex67 is essential to stabilize newly transcribed RNA before Xpo1-mediated nuclear export. The results further show that the Sm7 complex associates with and stabilizes the telomerase RNA in the cytoplasm and promotes its nuclear re-import. Remarkably, after this cytoplasmic passage, the nuclear stability of telomerase RNA no longer depends on Mex67. These results underscore the utility of inducible RNA tagging and challenge current models of telomerase maturation.


2004 ◽  
Vol 67 (7) ◽  
pp. 1187-1232 ◽  
Author(s):  
M Thoennessen
Keyword(s):  

2021 ◽  
pp. 002234332110185
Author(s):  
Francesco Bailo ◽  
Benjamin E Goldsmith

This article contributes to both the theoretical elaboration and empirical testing of the ‘stability–instability paradox’, the proposition that while nuclear weapons deter nuclear war, they also increase conventional conflict among nuclear-armed states. Some recent research has found support for the paradox, but quantitative studies tend to pool all international dyads while qualitative and theoretical studies focus almost exclusively on the USA–USSR and India–Pakistan dyads. This article argues that existing empirical tests lack clearly relevant counterfactual cases, and are vulnerable to a number of inferential problems, including selection on the dependent variable, unintentionally biased inference, and extrapolation from irrelevant cases. The limited evidentiary base coincides with a lack of consideration of the theoretical conditions under which the paradox might apply. To address these issues this article theorizes some scope conditions for the paradox. It then applies synthetic control, a quantitative method for valid comparison when appropriate counterfactual cases are lacking, to model international conflict between India–Pakistan, China–India, and North Korea–USA, before and after nuclearization. The article finds only limited support for the paradox when considered as a general theory, or within the theorized scope conditions based on the balance of resolve and power within each dyad.


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