The Study of Single Biomolecules with Fluorescence Methods

2001 ◽  
pp. 326-337 ◽  
Author(s):  
T. Ha ◽  
X. Zhuang ◽  
H. Babcock ◽  
H. Kim ◽  
J. W. Orr ◽  
...  
Keyword(s):  
Fluorescence Methods

Propylene Oxidation Mechanisms and Intermediates Using in Situ Soft X-ray Fluorescence Methods on the Pt(111) Surface

2000 ◽  
Vol 122 (1) ◽  
pp. 143-149 ◽  
Author(s):  
Aaron M. Gabelnick ◽  
Adam T. Capitano ◽  
Sean M. Kane ◽  
John L. Gland ◽  
Daniel A. Fischer
Keyword(s):  
Propylene Oxidation ◽  
X Ray ◽  
Fluorescence Methods ◽  
Oxidation Mechanisms

Fluorescence Methods in the Study of Polymer-Surfactant Systems

2020 ◽  
pp. 267-315
Author(s):  
Françoise M. Winnik ◽  
Sudarshi T.A. Regismond
Keyword(s):  
Fluorescence Methods ◽  
Surfactant Systems ◽  
Polymer Surfactant

scholarly journals Daptomycin Pore Formation and Stoichiometry Depend on Membrane Potential of Target Membrane

2018 ◽  
Vol 63 (1) ◽  
Author(s):  
Gabriela Seydlová ◽  
Albert Sokol ◽  
Petra Lišková ◽  
Ivo Konopásek ◽  
Radovan Fišer
Keyword(s):  
Membrane Potential ◽  
Lipid Bilayers ◽  
Pore Formation ◽  
Content Type ◽  
Calcium Dependent ◽  
Screening Experiment ◽  
Serious Infections ◽  
Fluorescence Methods ◽  
Initial Membrane ◽  
Target Membrane

ABSTRACT Daptomycin is a calcium-dependent lipodepsipeptide antibiotic clinically used to treat serious infections caused by Gram-positive pathogens. Its precise mode of action is somewhat controversial; the biggest issue is daptomycin pore formation, which we directly investigated here. We first performed a screening experiment using propidium iodide (PI) entry to Bacillus subtilis cells and chose the optimum and therapeutically relevant conditions (10 µg/ml daptomycin and 1.25 mM CaCl2) for the subsequent analyses. Using conductance measurements on planar lipid bilayers, we show that daptomycin forms nonuniform oligomeric pores with conductance ranging from 120 pS to 14 nS. The smallest conductance unit is probably a dimer; however, tetramers and pentamers occur in the membrane most frequently. Moreover, daptomycin pore-forming activity is exponentially dependent on the applied membrane voltage. We further analyzed the membrane-permeabilizing activity in B. subtilis cells using fluorescence methods [PI and DiSC3(5)]. Daptomycin most rapidly permeabilizes cells with high initial membrane potential and dissipates it within a few minutes. Low initial membrane potential hinders daptomycin pore formation.

scholarly journals Radiographic and Laser Fluorescence Methods Have No Benefits for Detecting Caries in Primary Teeth

2012 ◽  
Vol 46 (6) ◽  
pp. 536-543 ◽  
Author(s):  
F.M. Mendes ◽  
T.F. Novaes ◽  
R. Matos ◽  
D.G. Bittar ◽  
C. Piovesan ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Laser Fluorescence ◽  
Fluorescence Methods
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