The release of metals and other elements from dental and orthopedic implants into the tissues has been a matter of numerous studies. However, the conditions that favor such a phenomenon have not been completely understood so far. PIXE microbeam technique has been applied to study the spatial distribution of the elements released from the implants. Subjects of interest have been implants made from commercially pure titanium, Ti -6 Al -4 V alloy and 316 LWM surgical steel. Block sections of five dental implants, as well as tissues surrounding one metal spine plate and two hip prostheses, have been examined. Rabbit bones containing copper inclusions have been taken for microbeam analysis to complete the study about the metal release processes. Samples from the tissues and bone slices have been analyzed using PIXE microbeam setup at National Laboratories of Legnaro, Italy. Traces of the implant metals, including titanium but with the exception of vanadium, were found in the surrounding tissues and maps of their elemental distribution at different distances from the contact surface with implants have been obtained. The origin and the differences of the release rate of the observed metals have been discussed.