The Dynamics of Clathrin Coats in Living Cells Measured by Advanced Fluorescence Microscopy

Endocytosis ◽  
1992 ◽  
pp. 27-42
Author(s):  
Jean Davoust ◽  
Pierre Cosson
Keyword(s):  
Fluorescence Microscopy ◽  
Living Cells

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

scholarly journals Nonparametric detection of changes over time in image data from fluorescence microscopy of living cells

2021 ◽  
Author(s):  
Kathrin Bissantz ◽  
Nicolai Bissantz ◽  
Katharina Proksch
Keyword(s):  
Fluorescence Microscopy ◽  
Image Data ◽  
Living Cells ◽  
Nonparametric Detection ◽  
Changes Over Time ◽  
Over Time

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

Time-resolved fluorescence microscopy for quantitative Ca2+ imaging in living cells

2013 ◽  
Vol 405 (26) ◽  
pp. 8525-8537 ◽  
Author(s):  
Kristina Sagolla ◽  
Hans-Gerd Löhmannsröben ◽  
Carsten Hille
Keyword(s):  
Fluorescence Microscopy ◽  
Living Cells ◽  
Time Resolved Fluorescence ◽  
Time Resolved

scholarly journals Dual-Color Fluorescence Microscopy for Imaging DNA Loops in Living Cells

2011 ◽  
Vol 100 (3) ◽  
pp. 478a
Author(s):  
Joshua Milstein ◽  
Mike Chu ◽  
Jens-Christian Meiners
Keyword(s):  
Fluorescence Microscopy ◽  
Living Cells ◽  
Dna Loops ◽  
Dual Color

scholarly journals Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy

ACS Nano ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. 265-273 ◽  
Author(s):  
Nalan Liv ◽  
Daan S. B. van Oosten Slingeland ◽  
Jean-Pierre Baudoin ◽  
Pieter Kruit ◽  
David W. Piston ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Living Cells
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