Scanning Electrochemical and Fluorescence Microscopy for Detection of Reactive Oxygen Species in Living Cells

Author(s):  
Sean E. Salamifar ◽  
Rebecca Y. Lai
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...


2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).


2019 ◽  
Vol 10 (33) ◽  
pp. 7690-7694 ◽  
Author(s):  
Yiming Hu ◽  
Xiaoyi Li ◽  
Yu Fang ◽  
Wen Shi ◽  
Xiaohua Li ◽  
...  

A reactive oxygen species-triggered off-on fluorescence H2S donor is develop for the real-time imaging of H2S delivery and the cytoprotection against the hazardous oxidative environment.


The Analyst ◽  
2021 ◽  
Author(s):  
Fangyun Xin ◽  
Jiwei Zhao ◽  
Wei Shu ◽  
Xiaoling Zhang ◽  
Xixian Luo ◽  
...  

Peroxynitrite (ONOO‒), a highly reactive oxygen species (ROS), is implicated with many physiological and pathological processes including cancer, neurodegenerative diseases and inflammation. In this regard, developing effective tools for highly...


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