Saliva Collection Devices and Diagnostic Platforms

Introduction and Objective: Tooth decay is one of the most common chronic oral diseases found in industrial countries and is a multifactorial disease which has sugar as a key dietary factor. The amount of saliva concentration and presence of cariogenic bacteria will favor the development of caries. Because of this, the aim of this study was to collect and analyze data on oral alterations referred to tooth decay, oral pH changes, and changes of the oral microbiota in two distinct groups of workers. Material and methods: 30 individuals belonging to two different groups of workers: group A (GA) – workers who maintain daily contact with the confectionery; group B (GB) – workers who do not have such contact. Saliva collection was done by analysis of the salivary pH in both groups, as well as cultivation of Lactobacillus spp and S. mutans. We also evaluate the dental status of individuals belonging to the two groups through the DMFT index. Results: After the examinations of 30 workers (17 from the GA [9 men and 8 women] and 13 in the GB [7 men and 6 women]), the mean DMFT of the individuals in the group A and group B, was 7.41 (SD 5.14) 7.08 (SD 5.56), respectively, without statistically significant differences (p < 0.05). The count of S. mutans and Lactobacillus spp, was not statistically significant. Conclusion: There was no statistically significant relationship between presence of dental caries and the fact that workers are in contact with sugar because they work on candy food industry, but new studies are needed for more precise research.

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Surgical eye spears for saliva collection and secretory immunoglobulin A measurements

Journal of Oral Science ◽

10.2334/josnusd.15-0396 ◽

2016 ◽

Vol 58 (2) ◽

pp. 205-210

Author(s):

George N. Konstantinou ◽

Christina M. Cherny ◽

Anna Nowak-Węgrzyn

Keyword(s):

Immunoglobulin A ◽

Secretory Immunoglobulin A ◽

Saliva Collection ◽

Secretory Immunoglobulin

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Noninvasive Saliva Collection for DNA Analyses From Free-Ranging Tibetan Macaques (Macaca thibetana)

American Journal of Primatology ◽

10.1002/ajp.22062 ◽

2012 ◽

Vol 74 (11) ◽

pp. 1064-1070 ◽

Cited By ~ 8

Author(s):

N. D. SIMONS ◽

J. G. LORENZ ◽

L. K. SHEERAN ◽

J. H. LI ◽

D. P. XIA ◽

...

Keyword(s):

Macaca Thibetana ◽

Dna Analyses ◽

Saliva Collection ◽

Free Ranging

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Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502009000300021 ◽

2009 ◽

Vol 45 (3) ◽

pp. 551-557 ◽

Cited By ~ 2

Author(s):

Denise de Andrade ◽

Paula Regina de Souza ◽

Carolina Contador Beraldo ◽

Evandro Watanabe ◽

Mery Elen Lima ◽

...

Keyword(s):

Repeated Measures ◽

Chloride Solution ◽

Cetylpyridinium Chloride ◽

Strain Identification ◽

Diverse Range ◽

Significance Level ◽

Hospital Patients ◽

Saliva Collection ◽

Critical Patients ◽

Staphylococcus Spp

The use of mouthwashes in critical patients has been a source of concern for health professionals due to the diverse range of products, causing uncertainty about which is the most indicated. This study aimed to assess the susceptibility of Staphylococcus spp. isolated in the saliva of individuals from the community and patients in Intensive Care Units (ICU) as to antiseptic mouthwashes. The following oral antiseptics were assessed: cetylpyridinium chloride solution, Listerine® and Neen®. Calcium alginate swab was used for saliva collection to isolate Staphylococcus spp. Microbiological processing involved growth, isolation, strain identification and determination of MID (maximum inhibitory dilution). MID was considered the greatest dilution that completely inhibited the strains. The products efficacy was analyzed by a two-factor ANOVA repeated measures and by Bonferroni adjustments in multiple comparisons, considering a significance level of α=0.05. In total, 80 strains of Staphylococcus spp. were isolated, 40 from ICU patients and 40 from community individuals. MID results revealed that cetylpyridinium chloride solution presented better results in comparison to other products, that is, 39 (97.5%) strains from hospital patients with MID 1:128, and 37 (92.5%) of individuals from the community had MID 1:64. Neen® inhibited all strains in both groups at a dilution from 1:2 to 1:4. Listerine® presented the worst MID results, 65% of the strains from individuals from the community and 10% of hospital strains were not inhibited at a dilution of 1:2.

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Evaluation of saliva sampling procedures for SARS-CoV-2 diagnostics reveals differential sensitivity and association with viral load

10.1101/2020.10.06.20207902 ◽

2020 ◽

Author(s):

Pieter Mestdagh ◽

Michel Gillard ◽

Marc Arbyn ◽

Jean-Paul Pirnay ◽

Jeroen Poels ◽

...

Keyword(s):

Viral Load ◽

High Risk ◽

Health Professionals ◽

High Viral Load ◽

Sampling Procedure ◽

Differential Sensitivity ◽

Collection Method ◽

Viral Loads ◽

Saliva Collection ◽

Sampling Procedures

AbstractNasopharyngeal sampling has been the preferential collection method for SARS-CoV-2 diagnostics. Alternative sampling procedures that are less invasive and do not require a healthcare professional would be more preferable for patients and health professionals. Saliva collection has been proposed as such a possible alternative sampling procedure. We evaluated the sensitivity of SARS-CoV-2 testing on two different saliva collection devices (spitting versus swabbing) compared to nasopharyngeal swabs in over 2500 individuals that were either symptomatic or had high-risk contacts with infected individuals. We observed an overall poor sensitivity in saliva for SARS-CoV-2 detection (30.8% and 22.4% for spitting and swabbing, respectively). However, when focusing on individuals with medium to high viral load, sensitivity increased substantially (97.0% and 76.7% for spitting and swabbing, respectively), irrespective of symptomatic status. Our results suggest that saliva cannot readily replace nasopharyngeal sampling for SARS-CoV-2 diagnostics but may enable identification of cases with medium to high viral loads.

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Comparative evaluation of saliva collection methods for proteome analysis

Clinica Chimica Acta ◽

10.1016/j.cca.2013.01.013 ◽

2013 ◽

Vol 419 ◽

pp. 42-46 ◽

Cited By ~ 42

Author(s):

Claas Golatowski ◽

Manuela Gesell Salazar ◽

Vishnu Mukund Dhople ◽

Elke Hammer ◽

Thomas Kocher ◽

...

Keyword(s):

Comparative Evaluation ◽

Proteome Analysis ◽

Saliva Collection ◽

Collection Methods

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Comparison of Saliva Collection Methods in Children with High-Functioning Autism Spectrum Disorders: Acceptability and Recovery of Cortisol

Child Psychiatry & Human Development ◽

10.1007/s10578-012-0284-3 ◽

2012 ◽

Vol 43 (4) ◽

pp. 560-573 ◽

Cited By ~ 14

Author(s):

Susan K. Putnam ◽

Christopher Lopata ◽

Jeffery D. Fox ◽

Marcus L. Thomeer ◽

Jonathan D. Rodgers ◽

...

Keyword(s):

Autism Spectrum Disorders ◽

High Functioning Autism ◽

Autism Spectrum ◽

High Functioning ◽

Saliva Collection ◽

Spectrum Disorders ◽

Collection Methods

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INFLATABLE PLUG FOR RETICULO-OMASAL ORIFICE AND EQUIPMENT FOR SALIVA COLLECTION FROM FISTULATED STEERS

Canadian Journal of Animal Science ◽

10.4141/cjas58-011 ◽

1958 ◽

Vol 38 (1) ◽

pp. 85-86 ◽

Cited By ~ 3

Author(s):

H. M. Cunningham

Keyword(s):

Saliva Collection

not available

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Saliva is a Promising Alternative Specimen for the Detection of SARS-CoV-2 in Children and Adults

Journal of Clinical Microbiology ◽

10.1128/jcm.02686-20 ◽

2020 ◽

Author(s):

Rebecca Yee ◽

Thao Truong ◽

Pia S. Pannaraj ◽

Natalie Eubanks ◽

Emily Gai ◽

...

Keyword(s):

Health Care Workers ◽

Pediatric Patients ◽

Lower Cost ◽

Nasopharyngeal Swab ◽

Protective Equipment ◽

Promising Alternative ◽

Care Workers ◽

Saliva Collection ◽

Alternative Specimen ◽

Variable Performance

Testing efforts for SARS-CoV-2 have been burdened by the scarcity of testing materials and personal protective equipment for health care workers. The simple and painless process of saliva collection allows for widespread testing, but enthusiasm is hampered by variable performance compared to nasopharyngeal swab (NPS) samples. We prospectively collected paired NPS and saliva samples from a total of 300 unique adult and pediatric patients. SARS-CoV-2 RNA was detected in 32.2% (97/300) of the individuals using the TaqPath COVID-19 Combo Kit (Thermo Fisher). Performance of saliva and NPS were compared against the total number of positives regardless of specimen type. The overall concordance for saliva and NPS was 91.0% (273/300) and 94.7% (284/300), respectively. The positive percent agreement (PPA) for saliva and NPS was 81.4% (79/97) and 89.7% (87/97), respectively. Saliva detected 10 positive cases that were negative by NPS. In symptomatic and asymptomatic pediatric patients not previously diagnosed with COVID-19, the performances of saliva and NPS were comparable (PPA: 82.4% vs 85.3%). The overall PPA for adults were 83.3% and 90.7% for saliva and NPS, respectively, with saliva detecting 4 cases less than NPS. However, saliva performance in symptomatic adults was identical to NPS (PPA of 93.8%). With lower cost and self-collection capabilities, saliva can be an appropriate alternative sample choice to NPS for detection of SARS-CoV-2 in children and adults.

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