Identification and Characterization of a CA-CA1P Phosphate Exchange Activity in Leaf Extracts from French Bean (Phaseolus vulgaris L.)

1995 ◽  
pp. 4075-4078
Author(s):  
P. J. Andralojc ◽  
J. E. Ryan ◽  
M. A. J. Parry ◽  
A. J. Keys
Keyword(s):  
Phaseolus Vulgaris ◽  
French Bean ◽  
Phaseolus Vulgaris L ◽  
Leaf Extracts ◽  
Phosphate Exchange ◽  
Identification And Characterization ◽  
Exchange Activity

scholarly journals 2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.)

1996 ◽  
Vol 316 (2) ◽  
pp. 389-393 ◽  
Author(s):  
P. John ANDRALOJC ◽  
Jane E. RYAN ◽  
Martin A. J. PARRY ◽  
Alfred J. KEYS
Keyword(s):  
Phaseolus Vulgaris ◽  
Exchange Reaction ◽  
French Bean ◽  
Phosphate Group ◽  
Phaseolus Vulgaris L ◽  
Rate Enhancement ◽  
Bicarbonate Ions ◽  
Phosphate Donor ◽  
Phosphate Exchange

Transfer of the phosphate group of 2-carboxy-D-arabinitol 1-phosphate (CA1P) to 14C-labelled 2-carboxy-D-arabinitol (CA) was catalysed by extracts from leaves of Phaseolus vulgaris. This phosphotransferase activity co-purified with CA1P phosphatase, described previously. This activity was increased, up to 16-fold, by addition of bicarbonate ions at pH 9–10, suggesting rate enhancement by enzyme carbamylation. A Vmax of 1.5 μmol/min per mg of protein and a Km (for CA) of 1.8 mM were estimated for the exchange reaction, with the purified phosphatase. 2-Carboxy-D-arabinitol 1,5-bisphosphate and 2-carboxy-D-ribitol 1,5-bisphosphate, but not D-ribulose 1,5-bisphosphate, could replace CA1P as phosphate donor to [14C]CA.

Identification and Characterization of Phytohemagglutinins from White Kidney Beans (Phaseolus vulgaris L., var. Beldia) in the Rat Small Intestine

2016 ◽  
Vol 19 (1) ◽  
pp. 85-97 ◽  
Author(s):  
Nader Nciri ◽  
Namjun Cho ◽  
Faiçal El Mhamdi ◽  
Abderraouf Ben Mansour ◽  
Fayçal Haj Sassi ◽  
...  
Keyword(s):  
Small Intestine ◽  
Phaseolus Vulgaris ◽  
Rat Small Intestine ◽  
Phaseolus Vulgaris L ◽  
Kidney Beans ◽  
Identification And Characterization
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