Identification and Characterization of High Temperature Stress Responsive Novel miRNAs in French Bean (Phaseolus vulgaris)

2015 ◽  
Vol 176 (3) ◽  
pp. 835-849 ◽  
Author(s):  
M. N. Jyothi ◽  
D. V. Rai ◽  
R. Nagesh babu
Keyword(s):  
High Temperature ◽  
Phaseolus Vulgaris ◽  
Temperature Stress ◽  
High Temperature Stress ◽  
French Bean ◽  
Identification And Characterization

scholarly journals Photochemical efficiency in bean plants (Phaseolus vulgaris L. and Vigna unguiculata L. Walp) during recovery from high temperature stress

2002 ◽  
Vol 14 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Ernany Santos Costa ◽  
Ricardo Bressan-Smith ◽  
Jurandi Gonçalves de Oliveira ◽  
Eliemar Campostrini ◽  
Carlos Pimentel
Keyword(s):  
High Temperature ◽  
Phaseolus Vulgaris ◽  
Temperature Stress ◽  
Vigna Unguiculata ◽  
Photochemical Efficiency ◽  
High Temperature Stress ◽  
Reaction Centre ◽  
Phaseolus Vulgaris L ◽  
Light Harvesting Complex ◽  
Photochemical Efficiency Of Psii

Bean (Phaseolus vulgaris L., cv. Carioca and cv. Negro Huasteco) and cowpea plants (Vigna unguiculata L. Walp cv. Epace 10) were grown in a growth chamber with PPF at leaf level of 200 mumol.m-2.s-1 and air temperature 25 + 1 ºC. The first fully expanded pair of leaves of 12-day-old plants was submitted to high temperature stress (25, 30, 35, 40, 45 and 48 ºC) for 1.5 h. The photochemical efficiency of PSII during recovery was monitored by means of chlorophyll a fluorescence at six different times (0.5, 1, 2, 4, 24, and 48 h) after stress, at 25 ºC, using a modulated fluorimeter. Increasing temperature promoted an increase in Fphi at 45 ºC, possibly associated with dissociation of the light harvesting complex from the reaction centre of PSII, but a decrease was observed at 48 ºC in all cultivars. Fmax decreased at 48 ºC in Carioca and Negro Huasteco, but not in Epace 10, showing a possible correlation between heat tolerance and Fmax for this cultivar. The low values of Fmax in Carioca and Negro Huasteco indicated a loss of PSII activity followed by death of these plants. Fv/Fmax did not vary in Epace 10 but varied in Carioca and Negro Huasteco with increasing temperatures.

scholarly journals Molecular cloning and in-silico characterization of high temperature stress responsive pAPX gene isolated from heat tolerant Indian wheat cv. Raj 3765

2014 ◽  
Vol 7 (1) ◽  
Author(s):  
Jasdeep Chatrath Padaria ◽  
Harinder Vishwakarma ◽  
Koushik Biswas ◽  
Rahul Singh Jasrotia ◽  
Gyanendra Pratap Singh
Keyword(s):  
High Temperature ◽  
Molecular Cloning ◽  
Temperature Stress ◽  
In Silico ◽  
High Temperature Stress ◽  
Heat Tolerant ◽  
In Silico Characterization

scholarly journals Differential Modulation of Heat-Inducible Genes Across Diverse Genotypes and Molecular Cloning of a sHSP From Pearl Millet [Pennisetum glaucum (L.) R. Br.]

2021 ◽  
Vol 12 ◽  
Author(s):  
S. Mukesh Sankar ◽  
C. Tara Satyavathi ◽  
Sharmistha Barthakur ◽  
Sumer Pal Singh ◽  
C. Bharadwaj ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Expression Pattern ◽  
Pearl Millet ◽  
Temperature Stress ◽  
Expression Profiling ◽  
High Temperature Stress ◽  
Transcript Expression

The survival, biomass, and grain yield of most of the crops are negatively influenced by several environmental stresses. The present study was carried out by using transcript expression profiling for functionally clarifying the role of genes belonging to a small heat shock protein (sHSP) family in pearl millet under high-temperature stress. Transcript expression profiling of two high-temperature-responsive marker genes, Pgcp70 and PgHSF, along with physio-biochemical traits was considered to screen out the best contrasting genotypes among the eight different pearl millet inbred lines in the seedling stage. Transcript expression pattern suggested the existence of differential response among different genotypes upon heat stress in the form of accumulation of heat shock-responsive gene transcripts. Genotypes, such as WGI 126, TT-1, TT-6, and MS 841B, responded positively toward high-temperature stress for the transcript accumulation of both Pgcp70 and PgHSF and also indicated a better growth under heat stress. PPMI-69 showed the least responsiveness to transcript induction; moreover, it supports the membrane stability index (MSI) data for scoring thermotolerance, thereby suggesting the efficacy of transcript expression profiling as a molecular-based screening technique for the identification of thermotolerant genes and genotypes at particular crop growth stages. The contrasting genotypes, such as PPMI-69 (thermosusceptible) and WGI-126 and TT-1 (thermotolerant), are further utilized for the characterization of thermotolerance behavior of sHSP by cloning a PgHSP16.97 from the thermotolerant cv. WGI-126. In addition, the investigation was extended for the identification and characterization of 28 different HSP20 genes through a genome-wide search in the pearl millet genome and an understanding of their expression pattern using the RNA-sequencing (RNA-Seq) data set. The outcome of the present study indicated that transcript profiling can be a very useful technique for high-throughput screening of heat-tolerant genotypes in the seedling stage. Also, the identified PgHSP20s genes can provide further insights into the molecular regulation of pearl millet stress tolerance, thereby bridging them together to fight against the unpredicted nature of abiotic stress.

scholarly journals Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

2009 ◽  
Vol 7 (1) ◽  
pp. 33 ◽  
Author(s):  
Apiradee Hongsthong ◽  
Matura Sirijuntarut ◽  
Rayakorn Yutthanasirikul ◽  
Jittisak Senachak ◽  
Pavinee Kurdrid ◽  
...  
Keyword(s):  
High Temperature ◽  
Stress Response ◽  
Temperature Stress ◽  
Spirulina Platensis ◽  
High Temperature Stress

scholarly journals Characterization of high-temperature stress-tolerant tomato (Solanum lycopersicum L.) genotypes by biochemical analysis and expression profiling of heat-responsive genes

3 Biotech ◽  
2021 ◽  
Vol 11 (2) ◽  
Author(s):  
Suhas Gorakh Karkute ◽  
Waquar Akhter Ansari ◽  
Achuit Kumar Singh ◽  
Prabhakar Mohan Singh ◽  
Nagendra Rai ◽  
...  
Keyword(s):  
High Temperature ◽  
Solanum Lycopersicum ◽  
Temperature Stress ◽  
Expression Profiling ◽  
Biochemical Analysis ◽  
High Temperature Stress ◽  
Solanum Lycopersicum L
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