Male Sterile Facilitated Recurrent Selection

Author(s):  
Duane E. Falk
1996 ◽  
Vol 126 (4) ◽  
pp. 397-402 ◽  
Author(s):  
X. W. Wang ◽  
J. R. Lap ◽  
L Fan ◽  
R. B. Zhang

SUMMARYMass selection (MS) and bulk selection of a single character (CS) were used for the improvement of a base population, Bl, which was composed of Tai Gu male-sterile progenies of ten high protein content and four high yielding parental lines of wheat (Triticum aestivum L.), transferred by 6–10 backcrossing generations, with equal numbers of plants from each line. Backcross mass selection (BS) was used for the improvement of a second base population, B2, which was formed by Tai Gu malesterile plants of four high yielding parental lines, again with equal numbers of plants from each line. Three cycles of selection were conducted for both population Bl and B2. Direct genetic advances of sterile plants for six traits were studied. The results indicated that use of the Tai Gu male-sterile single dominant gene is effective for recurrent selection of wheat. For improvement of a single character, CS was the most effective method, of which the selective effect in the first cycle (Cl) was greater than in the second (C2) and the third cycle (C3), although multivariate analysis snowed that the CS method was not conducive to the maintenance of variation in a population. The greatest response to selection was found in C2 with the MS method. Multivariate analysis also indicated that both the MS and BS methods increased the variation in the population, but MS was found to be the best selective method as judged by the mean response over three cycles of selection.


1996 ◽  
Vol 121 (4) ◽  
pp. 604-608 ◽  
Author(s):  
Michael J. Havey ◽  
William M. Randle

A factorial mating design, using three male-sterile F1 lines in testcrosses with a sample of open-pollinated (OP) onion populations, was used to estimate combining abilities and heterosis for bulb yield, size, storage ability, pungency, soluble solids content (SSC), and water loss after 3 months in storage. Samples of testcross bulbs were flowered and scored for fertility to estimate frequencies of the nuclear allele maintaining cytoplasmic male sterility. General combining ability (GCA) estimates for OP populations (males) were significant (P < 0.05) for yield, SSC, and proportion of bulbs with diameters >7.5 cm. GCA estimates for female testers were significant for storage ability and proportion of bulbs with diameters <5.0 cm. Male × female interactions (specific combining ability estimates) were significant for SSC and storage ability. Our analyses did not reveal any storage population from which inbreds would likely yield significantly better with the male-sterile tester lines. Spanish OP populations tended to produce testcrosses with larger bulbs, lower pungency and SSC, and poorer storage ability. Heterosis estimates were most often significant for yield and SSC; less often for pungency, storage ability, and bulb size; and not significant for water loss in storage. Overall, significant GCA estimates indicate that superior onion inbreds and populations may be developed using recurrent-selection strategies that increase the frequency of desirable alleles with additive effects.


Genome ◽  
2009 ◽  
Vol 52 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Wenguang Cao ◽  
Daryl J. Somers ◽  
George Fedak

A male sterile wheat mutant, Triticum aestivum L. ‘Taigu’, was found in a wheat field in China in 1972. The male sterility was controlled by a single dominant gene that was referred to as Ms2. Recently, this gene was found to be linked to a dwarfing gene through crossing Taigu with the short wheat T. aestivum ‘Ai-Bian 1’ carrying the dwarfing gene Rht-D1c. The objective of this study was to develop molecular markers linked to the male sterility Ms2 gene in common wheat. One hundred and twenty-two near-isogenic lines were developed through backcrossing and sib intercrossing and used as the mapping population for the development of molecular markers. Bulked segregant analysis was used to screen 48 pairs of SSR primers, and a marker, MS2-WMC617, was identified closely linked to the male sterile Ms2 gene that mapped at the distal position of chromosome arm 4DS. The use of the molecular marker MS2-WMC617 can facilitate recurrent selection in a wheat breeding program based on marker-assisted selection.


2008 ◽  
Vol 59 (5) ◽  
pp. 470 ◽  
Author(s):  
E. L. Heffner ◽  
O. Chomdej ◽  
K. R. Williams ◽  
M. E. Sorrells

The introgression of exotic wheat germplasm such as synthetic hexaploid (tetraploid × diploid amphiploid) wheat (Triticum aestivum L.) into an adapted gene pool has the potential to amplify the genetic variation for complex traits. The dominant male-sterile gene can be used to facilitate recurrent selection in wheat, thus increasing the opportunity for genetic recombination between exotic and cultivated genomes and the identification and retention of desirable alleles. Our dominant male-sterile recurrent selection project began in 1983 with the intermating of the source germplasm (Chris hard red spring wheat in Triticum tauschii cytoplasm) with 34 soft winter lines and varieties, intermated without selection for 3 generations, and then selecting male-sterile plants for agronomic type each season. After 20 generations of recurrent selection, we extracted 94 inbred lines without selection during inbreeding except for fertility. Those lines were evaluated for linkage disequilibrium (LD) on the 5A chromosome for comparison with an earlier study involving 95 elite soft winter wheat varieties of similar genetic background. For the male-sterile population-derived lines, LD decayed within 1 cM (r2 < 0.1) compared with 5 cM in the previous report for soft winter wheat varieties. This approach to introgression facilitates the potential use of synthetic wheat and other unadapted germplasm for expanding the gene pool and provides a potential resource for high-resolution association mapping of genes controlling traits of interest.


2020 ◽  
Vol 183 (3) ◽  
pp. 243-254
Author(s):  
S H ABOU KHADRAH ◽  
M I ABO YOUSSEF ◽  
E M HAFEZ ◽  
A A REHAN

The main objective of study is evaluated some new inbred rice and male sterile varieties under Distinct, Uniform and Stability (DUS) and Value of Cultivated and Used (VCU) Experiments. A number of seven rice varieties were used, Sakha 101, Sakha 102, Sakha 104, Giza 177, Giza 178, Giza182 and Egyptian Yasmine; moreover, GZ. 10154 and GZ 8564-Sp 70, as well as EGMS and CMS1 as promising lines to evaluating by DUS and VCU. The data were recorded on morphological and yield characters. The results could be concluded that: regarding to qualitative characteristics (PQ and QL), from 24 PQ and QL characters, the nine rice varieties recorded the same score for 19 characters; moreover, the rice varieties Egyptian jasmine was dissimilar in the score No. 24, 39, and 60, that referred to this variety belong to Indica type while the rice varieties Sakha 101and Giza 178 were similar only in score no.11, which belong to Japonica and Indica japonica types, that meaning these varieties were highly uniform and stability in qualitative characters than the other promising line GZ10154. Regarding to quantities characteristics (QN), from 27 QN characters, 9 rice varieties recorded the same score for 20 characters; moreover, the rice varieties Sakha 101, Sakha 102, Sakha 104, Giza 177, Giza 178, Giza 182, Egyptian yasmine and GZ 8564-Sp70 were similar in the score no. 1, 12, 23, 48, 49 and 50, while the promising line GZ 10154 was dissimilar in these scores during the two seasons, meaning that all these varieties were highly uniform and stability than the other promising, line GZ 10154. These results were conformed to VCU results, where the studied varieties recorded the highest grain yield/day. From these results could be concluded that all the varieties, except GZ 10154, accepted as a new rice release variety, but the promising line GZ 10154 required to more recurrent selection to increase their uniform, as well as CMS line was accepted could be evaluated under different conditions, but EGMS should be evaluated under heat stress conditions.


2017 ◽  
Vol 8 ◽  
Author(s):  
Afsana Ansari ◽  
Chunlian Wang ◽  
Jian Wang ◽  
Fujun Wang ◽  
Piqing Liu ◽  
...  

2008 ◽  
Vol 59 (11) ◽  
pp. 999 ◽  
Author(s):  
J. A. G. Irwin ◽  
P. M. Pepper ◽  
D. Armour ◽  
J. M. Mackie ◽  
K. F. Lowe

Yield stagnation is a worldwide issue for lucerne breeding, and reasons for the yield plateau include emphasis on disease and pest resistance and not yield per se, and the broad-based synthetic approach to lucerne breeding which is generally used. In this study, an incomplete diallel was made between 50 lucerne clones with representatives from the 3 hypothetical heterotic groups, Medicago sativa subsp. falcata, dormant subsp. sativa, and non-dormant subsp. sativa. Male sterile clones were also included among the dormant group. The single crosses were compared in a subtropical environment at Gatton, Queensland, for yield and other relevant agronomic traits against the adapted synthetics Sequel (dormancy group 9), UQL-1 (group 7), and a highly non-dormant experimental synthetic (line B) derived by introgression of highly non-dormant Arabian germplasm into Sequel. The trial was conducted in a known low-disease-pressure site for Phytophthora root rot, and anthracnose was managed by regular application of prophylactic treatments. The best single cross outyielded Sequel and line B by 13% and 8%, respectively. In this environment, yield was very much influenced by the dormancy group of the test material, with group 9 material significantly outyielding more dormant material. General combining ability (GCA) effects were more important determinants of cumulative yields than specific combining ability (SCA) effects, with these effects being significantly greater than zero for only 4 of the 236 crosses tested over the 15-month period. Similarly, GCA effects were more important for determining autumn height and persistence. The research did identify a small number of clones with good GCA for yield per se, and it would appear that future work should focus on developing more narrow-based synthetics with 4–8 parents which have been selected on the basis of their GCA for yield per se. DNA markers would appear to have a role in selecting clones carrying multiple resistances, and in establishing marker pedigrees for high-yielding parental clones such as we have identified, which can be traced through subsequent generations of recurrent selection in cultivar improvement.


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