A molecular marker closely linked to the region of Rht-D1c and Ms2 genes in common wheat (Triticum aestivum)

Genome ◽  
2009 ◽  
Vol 52 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Wenguang Cao ◽  
Daryl J. Somers ◽  
George Fedak
Keyword(s):  
Triticum Aestivum ◽  
Molecular Markers ◽  
Molecular Marker ◽  
Male Sterility ◽  
Common Wheat ◽  
Recurrent Selection ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Male Sterile ◽  
Dwarfing Gene

A male sterile wheat mutant, Triticum aestivum L. ‘Taigu’, was found in a wheat field in China in 1972. The male sterility was controlled by a single dominant gene that was referred to as Ms2. Recently, this gene was found to be linked to a dwarfing gene through crossing Taigu with the short wheat T. aestivum ‘Ai-Bian 1’ carrying the dwarfing gene Rht-D1c. The objective of this study was to develop molecular markers linked to the male sterility Ms2 gene in common wheat. One hundred and twenty-two near-isogenic lines were developed through backcrossing and sib intercrossing and used as the mapping population for the development of molecular markers. Bulked segregant analysis was used to screen 48 pairs of SSR primers, and a marker, MS2-WMC617, was identified closely linked to the male sterile Ms2 gene that mapped at the distal position of chromosome arm 4DS. The use of the molecular marker MS2-WMC617 can facilitate recurrent selection in a wheat breeding program based on marker-assisted selection.

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

scholarly journals INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

2011 ◽  
Vol 24 (1) ◽  
pp. 33-40
Author(s):  
M. J. Hasan ◽  
M. U. Kulsum ◽  
A. Ansari ◽  
A. K. Paul ◽  
P. L. Biswas
Keyword(s):  
Oryza Sativa ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Gene Action ◽  
Oryza Sativa L ◽  
Fertility Restoration ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Male Sterile ◽  
Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.DOI: http://dx.doi.org/10.3329/bjpbg.v24i1.16997

scholarly journals The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

2019 ◽  
Vol 20 (24) ◽  
pp. 6252 ◽  
Author(s):  
Junchang Li ◽  
Jing Zhang ◽  
Huijuan Li ◽  
Hao Niu ◽  
Qiaoqiao Xu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Male Sterility ◽  
Genetic Research ◽  
Triticum Aestivum L ◽  
Anther Development ◽  
Sucrose Metabolism ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Microspore Stage ◽  
Regulation Model

Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.

Inheritance of the blue aleurone trait in diverse wheat crosses

Genome ◽  
1990 ◽  
Vol 33 (4) ◽  
pp. 525-529 ◽  
Author(s):  
V. D. Keppenne ◽  
P. S. Baenziger
Keyword(s):  
Triticum Aestivum ◽  
Genetic Marker ◽  
Dominant Gene ◽  
Triticum Aestivum L ◽  
Progeny Testing ◽  
Seed Color ◽  
Male Sterile ◽  
Agropyron Elongatum ◽  
Doubled Haploidy ◽  
Wheat Lines

The blue aleurone trait has been suggested as a useful genetic marker in wheat (Triticum aestivum L.). However, little information is available on its transmission in diverse backgrounds and on its use to identify hybrid seed. UC66049, a hexaploid spring wheat with a spontaneous translocation that included the gene for the blue aleurone trait (Ba) from Agropyron elongatum (Host) P.B. (synonymous with Elytrigia pontica (Podp.) Holub), was crossed to seven wheat cultivars to test the transmission of the trait. UC66049 was crossed to male-sterile red wheat lines to evaluate the blue aleurone trait as a marker for confirming hybridity. Ba segregated as a dominant gene that was transmitted normally through the male and female gametes. For 6 of 7 crosses with diverse pedigrees, we experienced problems with misclassification of the aleurone color in the F2 seed generation, determined by the F3 seed family data. The blue aleurone trait is a good genetic marker; however, progeny testing may be needed to confirm the F2 genotypes in some environments or genetic backgrounds. Moreover, Ba is useful in determining the amount of controlled hybridity as opposed to self-fertility and (or) outcrossing in genetic male-sterile wheat lines. The use of Ba to confirm doubled haploidy was proposed.Key words: Agropyron elongatum, seed color, genetics, Triticum aestivum, Elytrigia pontica.

scholarly journals Identification of Ms2, a Novel Locus Controlling Male-fertility Restoration of Cytoplasmic Male-sterility in Onion (Allium Cepa L.), and Development of Tightly Linked Molecular Markers

2021 ◽  
Author(s):  
Nari Yu ◽  
Sunggil Kim
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Allium Cepa ◽  
Male Fertility ◽  
Bulked Segregant Analysis ◽  
Fertility Restoration ◽  
Chromosome 2 ◽  
Rna Seq ◽  
Male Sterile ◽  
Male Fertility Restoration

Abstract Cytoplasmic male-sterility (CMS) has been exclusively used to produce F1 hybrid seeds of onion (Allium cepa L.). A single nuclear locus, Ms, is known to restore male-fertility of CMS in onions. Unstable male-sterile onions producing a small amount of pollen grains have been identified in a previous study. When such unstable male-sterile onions were crossed with stable male-sterile onions containing CMS-T cytoplasm, male-fertility was completely restored, although genotypes of the Ms locus were homozygous recessive. Inheritance patterns indicated that male-fertility restoration was controlled by a single locus designated as Ms2. A combined approach of bulked segregant analysis and RNA-seq was used to identify candidate genes for the Ms2 locus. High resolution melting (HRM) markers were developed based on single nucleotide polymorphisms (SNPs) detected by RNA-Seq. Comparative mapping of the Ms2 locus showed that Ms2 was positioned at the end of chromosome 2 with a distance of approximately 70 cM away from the Ms locus. Although 38 contigs containing reliable SNPs were analyzed using recombinants selected from 1,344 individuals, no contig showed perfect linkage to Ms2. Interestingly, transcription levels of orf725, a CMS-associated gene in onions, were significantly reduced in male-fertile individuals of segregating populations. However, no significant change in its transcription level was observed in individuals of a segregating population with male-fertility phenotypes determined by the Ms locus, suggesting that male-fertility restoration mechanism of Ms2 might be different from that of the Ms locus.

Phenotype characterisation and analysis of expression patterns of genes related mainly to carbohydrate metabolism and sporopollenin in male-sterile anthers induced by high temperature in wheat (Triticum aestivum)

2018 ◽  
Vol 69 (5) ◽  
pp. 469 ◽  
Author(s):  
Hongzhan Liu ◽  
Junsheng Wang ◽  
Chaoqiong Li ◽  
Lin Qiao ◽  
Xueqin Wang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
High Temperature ◽  
Male Sterility ◽  
Carbohydrate Metabolism ◽  
Higher Plants ◽  
Expression Patterns ◽  
Triticum Aestivum L ◽  
Male Sterile ◽  
Tetrad Stage ◽  
Expression Levels

Male reproductive development in higher plants is highly sensitive to various stressors, including high temperature (HT). In this study, physiological male-sterile plants of wheat (Triticum aestivum L.) were established using HT induction. The physiological changes and expression levels of genes mainly related to carbohydrate metabolism and sporopollenin in male-sterile processes were studied by using biological techniques, including iodine–potassium iodide staining, paraffin sectioning, scanning electron microscopy (SEM) and fluorescent quantitative analysis. Results of paraffin sectioning and SEM revealed that parts of HT male-sterile anthers, including the epidermis and tapetum, were remarkably different from those of normal anthers. The expression levels of TaSUT1, TaSUT2, IVR1 and IVR5 were significantly lower than of normal anthers at the early microspore and trinucleate stages. The RAFTIN1 and TaMS26 genes may contribute to biosynthesis and proper ‘fixation’ of sporopollenin in the development of pollen wall; however, their expression levels were significantly higher at the early tetrad stage and early microspore stage in HT sterile anthers. The recently cloned MS1 gene was expressed at the early tetrad and early microspore stages but not at the trinucleate stage. Moreover, this gene showed extremely significant, high expression in HT sterile anthers compared with normal anthers. These results demonstrate that HT induction of wheat male sterility is probably related to the expression of genes related to carbohydrate metabolism and sporopollenin metabolism. This provides a theoretical basis and technological approach for further studies on the mechanisms of HT induction of male sterility.

Effects of recurrent selection on populations of various generations in wheat by using the Tai Gu single dominant male-sterile gene

1996 ◽  
Vol 126 (4) ◽  
pp. 397-402 ◽  
Author(s):  
X. W. Wang ◽  
J. R. Lap ◽  
L Fan ◽  
R. B. Zhang
Keyword(s):  
Multivariate Analysis ◽  
Recurrent Selection ◽  
Dominant Gene ◽  
Dominant Male ◽  
Mass Selection ◽  
Base Population ◽  
Male Sterile ◽  
Single Character ◽  
Parental Lines ◽  
Selection Of

SUMMARYMass selection (MS) and bulk selection of a single character (CS) were used for the improvement of a base population, Bl, which was composed of Tai Gu male-sterile progenies of ten high protein content and four high yielding parental lines of wheat (Triticum aestivum L.), transferred by 6–10 backcrossing generations, with equal numbers of plants from each line. Backcross mass selection (BS) was used for the improvement of a second base population, B2, which was formed by Tai Gu malesterile plants of four high yielding parental lines, again with equal numbers of plants from each line. Three cycles of selection were conducted for both population Bl and B2. Direct genetic advances of sterile plants for six traits were studied. The results indicated that use of the Tai Gu male-sterile single dominant gene is effective for recurrent selection of wheat. For improvement of a single character, CS was the most effective method, of which the selective effect in the first cycle (Cl) was greater than in the second (C2) and the third cycle (C3), although multivariate analysis snowed that the CS method was not conducive to the maintenance of variation in a population. The greatest response to selection was found in C2 with the MS method. Multivariate analysis also indicated that both the MS and BS methods increased the variation in the population, but MS was found to be the best selective method as judged by the mean response over three cycles of selection.

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