Engineered Dwarf Male-Sterile Rice: A Promising Genetic Tool for Facilitating Recurrent Selection in Rice

SUMMARYMass selection (MS) and bulk selection of a single character (CS) were used for the improvement of a base population, Bl, which was composed of Tai Gu male-sterile progenies of ten high protein content and four high yielding parental lines of wheat (Triticum aestivum L.), transferred by 6–10 backcrossing generations, with equal numbers of plants from each line. Backcross mass selection (BS) was used for the improvement of a second base population, B2, which was formed by Tai Gu malesterile plants of four high yielding parental lines, again with equal numbers of plants from each line. Three cycles of selection were conducted for both population Bl and B2. Direct genetic advances of sterile plants for six traits were studied. The results indicated that use of the Tai Gu male-sterile single dominant gene is effective for recurrent selection of wheat. For improvement of a single character, CS was the most effective method, of which the selective effect in the first cycle (Cl) was greater than in the second (C2) and the third cycle (C3), although multivariate analysis snowed that the CS method was not conducive to the maintenance of variation in a population. The greatest response to selection was found in C2 with the MS method. Multivariate analysis also indicated that both the MS and BS methods increased the variation in the population, but MS was found to be the best selective method as judged by the mean response over three cycles of selection.

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Combining Abilities for Yield and Bulb Quality among Long- and Intermediate-day Open-pollinated Onion Populations

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.4.604 ◽

1996 ◽

Vol 121 (4) ◽

pp. 604-608 ◽

Cited By ~ 13

Author(s):

Michael J. Havey ◽

William M. Randle

Keyword(s):

Water Loss ◽

Combining Ability ◽

Recurrent Selection ◽

Soluble Solids ◽

Male Sterile ◽

Combining Abilities ◽

Selection Strategies ◽

Factorial Mating ◽

Solids Content ◽

Storage Ability

A factorial mating design, using three male-sterile F1 lines in testcrosses with a sample of open-pollinated (OP) onion populations, was used to estimate combining abilities and heterosis for bulb yield, size, storage ability, pungency, soluble solids content (SSC), and water loss after 3 months in storage. Samples of testcross bulbs were flowered and scored for fertility to estimate frequencies of the nuclear allele maintaining cytoplasmic male sterility. General combining ability (GCA) estimates for OP populations (males) were significant (P < 0.05) for yield, SSC, and proportion of bulbs with diameters >7.5 cm. GCA estimates for female testers were significant for storage ability and proportion of bulbs with diameters <5.0 cm. Male × female interactions (specific combining ability estimates) were significant for SSC and storage ability. Our analyses did not reveal any storage population from which inbreds would likely yield significantly better with the male-sterile tester lines. Spanish OP populations tended to produce testcrosses with larger bulbs, lower pungency and SSC, and poorer storage ability. Heterosis estimates were most often significant for yield and SSC; less often for pungency, storage ability, and bulb size; and not significant for water loss in storage. Overall, significant GCA estimates indicate that superior onion inbreds and populations may be developed using recurrent-selection strategies that increase the frequency of desirable alleles with additive effects.

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A molecular marker closely linked to the region of Rht-D1c and Ms2 genes in common wheat (Triticum aestivum)

Genome ◽

10.1139/g08-097 ◽

2009 ◽

Vol 52 (1) ◽

pp. 95-99 ◽

Cited By ~ 6

Author(s):

Wenguang Cao ◽

Daryl J. Somers ◽

George Fedak

Keyword(s):

Triticum Aestivum ◽

Molecular Markers ◽

Molecular Marker ◽

Male Sterility ◽

Common Wheat ◽

Recurrent Selection ◽

Bulked Segregant Analysis ◽

Dominant Gene ◽

Male Sterile ◽

Dwarfing Gene

A male sterile wheat mutant, Triticum aestivum L. ‘Taigu’, was found in a wheat field in China in 1972. The male sterility was controlled by a single dominant gene that was referred to as Ms2. Recently, this gene was found to be linked to a dwarfing gene through crossing Taigu with the short wheat T. aestivum ‘Ai-Bian 1’ carrying the dwarfing gene Rht-D1c. The objective of this study was to develop molecular markers linked to the male sterility Ms2 gene in common wheat. One hundred and twenty-two near-isogenic lines were developed through backcrossing and sib intercrossing and used as the mapping population for the development of molecular markers. Bulked segregant analysis was used to screen 48 pairs of SSR primers, and a marker, MS2-WMC617, was identified closely linked to the male sterile Ms2 gene that mapped at the distal position of chromosome arm 4DS. The use of the molecular marker MS2-WMC617 can facilitate recurrent selection in a wheat breeding program based on marker-assisted selection.

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Investigating Pollen and Gene Flow of WYMV-Resistant Transgenic Wheat N12-1 Using a Dwarf Male-Sterile Line as the Pollen Receptor

PLoS ONE ◽

10.1371/journal.pone.0151373 ◽

2016 ◽

Vol 11 (3) ◽

pp. e0151373 ◽

Cited By ~ 8

Author(s):

Shanshan Dong ◽

Yan Liu ◽

Cigang Yu ◽

Zhenhua Zhang ◽

Ming Chen ◽

...

Keyword(s):

Gene Flow ◽

Transgenic Wheat ◽

Dwarf Male ◽

Male Sterile ◽

Male Sterile Line

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Dominant male-sterile populations for association mapping and introgression of exotic wheat germplasm

Australian Journal of Agricultural Research ◽

10.1071/ar07221 ◽

2008 ◽

Vol 59 (5) ◽

pp. 470 ◽

Cited By ~ 4

Author(s):

E. L. Heffner ◽

O. Chomdej ◽

K. R. Williams ◽

M. E. Sorrells

Keyword(s):

Winter Wheat ◽

Association Mapping ◽

Gene Pool ◽

Recurrent Selection ◽

Dominant Male ◽

Male Sterile ◽

Wheat Varieties ◽

Wheat Germplasm ◽

Soft Winter Wheat ◽

Winter Wheat Varieties

The introgression of exotic wheat germplasm such as synthetic hexaploid (tetraploid × diploid amphiploid) wheat (Triticum aestivum L.) into an adapted gene pool has the potential to amplify the genetic variation for complex traits. The dominant male-sterile gene can be used to facilitate recurrent selection in wheat, thus increasing the opportunity for genetic recombination between exotic and cultivated genomes and the identification and retention of desirable alleles. Our dominant male-sterile recurrent selection project began in 1983 with the intermating of the source germplasm (Chris hard red spring wheat in Triticum tauschii cytoplasm) with 34 soft winter lines and varieties, intermated without selection for 3 generations, and then selecting male-sterile plants for agronomic type each season. After 20 generations of recurrent selection, we extracted 94 inbred lines without selection during inbreeding except for fertility. Those lines were evaluated for linkage disequilibrium (LD) on the 5A chromosome for comparison with an earlier study involving 95 elite soft winter wheat varieties of similar genetic background. For the male-sterile population-derived lines, LD decayed within 1 cM (r2 < 0.1) compared with 5 cM in the previous report for soft winter wheat varieties. This approach to introgression facilitates the potential use of synthetic wheat and other unadapted germplasm for expanding the gene pool and provides a potential resource for high-resolution association mapping of genes controlling traits of interest.

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EVALUATE SOME NEW INBRED RICE AND MALE STERILE VARIETIES UNDER DUS AND VCU EXPERIMENTS

Cercetari Agronomice in Moldova ◽

10.46909/10.46909/cerce-2020-021 ◽

2020 ◽

Vol 183 (3) ◽

pp. 243-254

Author(s):

S H ABOU KHADRAH ◽

M I ABO YOUSSEF ◽

E M HAFEZ ◽

A A REHAN

Keyword(s):

Heat Stress ◽

Recurrent Selection ◽

The Other ◽

Male Sterile ◽

Rice Varieties ◽

Cms Line ◽

Qualitative Characters ◽

Promising Line ◽

Highly Uniform ◽

Qualitative Characteristics

The main objective of study is evaluated some new inbred rice and male sterile varieties under Distinct, Uniform and Stability (DUS) and Value of Cultivated and Used (VCU) Experiments. A number of seven rice varieties were used, Sakha 101, Sakha 102, Sakha 104, Giza 177, Giza 178, Giza182 and Egyptian Yasmine; moreover, GZ. 10154 and GZ 8564-Sp 70, as well as EGMS and CMS1 as promising lines to evaluating by DUS and VCU. The data were recorded on morphological and yield characters. The results could be concluded that: regarding to qualitative characteristics (PQ and QL), from 24 PQ and QL characters, the nine rice varieties recorded the same score for 19 characters; moreover, the rice varieties Egyptian jasmine was dissimilar in the score No. 24, 39, and 60, that referred to this variety belong to Indica type while the rice varieties Sakha 101and Giza 178 were similar only in score no.11, which belong to Japonica and Indica japonica types, that meaning these varieties were highly uniform and stability in qualitative characters than the other promising line GZ10154. Regarding to quantities characteristics (QN), from 27 QN characters, 9 rice varieties recorded the same score for 20 characters; moreover, the rice varieties Sakha 101, Sakha 102, Sakha 104, Giza 177, Giza 178, Giza 182, Egyptian yasmine and GZ 8564-Sp70 were similar in the score no. 1, 12, 23, 48, 49 and 50, while the promising line GZ 10154 was dissimilar in these scores during the two seasons, meaning that all these varieties were highly uniform and stability than the other promising, line GZ 10154. These results were conformed to VCU results, where the studied varieties recorded the highest grain yield/day. From these results could be concluded that all the varieties, except GZ 10154, accepted as a new rice release variety, but the promising line GZ 10154 required to more recurrent selection to increase their uniform, as well as CMS line was accepted could be evaluated under different conditions, but EGMS should be evaluated under heat stress conditions.

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