Double Immunoenzyme Staining Methods with Special Reference to Monoclonal Antibodies.

1987 ◽  
pp. 37-47 ◽  
Author(s):  
H. Mullink ◽  
D. M. Boorsma ◽  
S. C. Henzen-Logmans ◽  
C. J. L. M. Meijer
Keyword(s):  
Monoclonal Antibodies ◽  
Special Reference ◽  
Staining Methods

scholarly journals Evaluation of E-rosetting human lymphocytes with OKT11 and other monoclonal antibodies

Blood ◽  
1982 ◽  
Vol 60 (3) ◽  
pp. 795-799 ◽  
Author(s):  
SH Ip ◽  
CW Rittershaus ◽  
CC Struzziero ◽  
JA Hoxie ◽  
RA Hoffman ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescence Intensity ◽  
Binding Sites ◽  
Blood Cells ◽  
Human Lymphocytes ◽  
Immunofluorescent Staining ◽  
Intensity Data ◽  
Blood Samples ◽  
Staining Methods

Abstract Monoclonal antibody OKT11 was found to compete with sheep red blood cells for binding sites on human lymphocytes. Preincubation of lymphocytes with OKT11 eliminated E-rosette formation. In a study of 142 peripheral blood samples ranging from 1% to over 90% E-rosette- positive cells, comparison to the percent OKT11-positive cells yielded a correlation coefficient of 0.93. In normal donors, subsets of OKT11+ cells were identified using two-color immunofluorescent staining methods with OKT3, OKT4, and OKT8. On the average, approximately 13% of OKT11+ lymphocytes were OKT3- and 13% of OKT11+ lymphocytes were OKT4- and OKT8-. Based on our double antibody fluorescence intensity data, low antigen density OKT11+ lymphocytes were OKT3-. OKT4+ and OKT8+ lymphocytes in normal peripheral lymphocytes have similar OKT11 antigen density.

scholarly journals Immunocytochemical Labelling of Haematological Samples Using Monoclonal Antibodies

Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 127
Author(s):  
Wendy N. Erber
Keyword(s):  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
University Of Oxford ◽  
Alkaline Phosphatase Staining ◽  
Nuffield Department ◽  
Staining Methods ◽  
Biological Discovery ◽  
The University ◽  
Productive Time

I reflect on my experience working with David Y. Mason in the Leukaemia Research Laboratories in the Nuffield Department of Pathology at the University of Oxford in the early 1980s. This was soon after the first monoclonal antibodies had been produced, which led to an exciting and productive time in biological discovery and pathology diagnostics. A specific focus in the laboratory was the development of immunoenzymatic staining methods that would enable monoclonal antibodies to be applied in diagnostic practice. This paper describes the work that led to the performance of immuno-alkaline phosphatase staining on blood and bone marrow smears, the success of which changed leukaemia diagnosis.

Conservation of feline semen: Part II: Cold-induced damages on spermatozoal fertilizing ability

2003 ◽  
Vol 5 (5) ◽  
pp. 257-263 ◽  
Author(s):  
G.C Luvoni ◽  
E Kalchschmidt ◽  
G Marinoni
Keyword(s):  
Special Reference ◽  
Functional Status ◽  
Limiting Factor ◽  
Fluorescent Staining ◽  
Epididymal Sperm ◽  
Freeze Thaw ◽  
Functional Integrity ◽  
Sperm Characteristics ◽  
Fertilizing Ability ◽  
Staining Methods

During the conservation of feline semen, the freeze–thaw procedure in particular is responsible for inducing severe spermatozoal damage, which diminishes fertilizing ability. Therefore, cold-induced damage represents a limiting factor for the conservation of semen, particularly semen from felids, which are often affected by teratospermia. In this article, feline sperm characteristics are reported, with special reference to motility and morphology, which are more likely to be affected by conservation protocols; and moreover, the causes of cold-induced damages are described. Attention has been focused on methods to evaluate functional integrity of spermatozoa, and those applied to cat semen are reviewed. Among these, a rather recently developed technique involves fluorescent staining methods, and in particular chlortetracycline. The chlortetracycline assay applied to cryopreserved cat epididymal sperm shows that it is suitable to evaluate the functional status of cat sperm.

scholarly journals Evaluation of E-rosetting human lymphocytes with OKT11 and other monoclonal antibodies

Blood ◽  
1982 ◽  
Vol 60 (3) ◽  
pp. 795-799
Author(s):  
SH Ip ◽  
CW Rittershaus ◽  
CC Struzziero ◽  
JA Hoxie ◽  
RA Hoffman ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescence Intensity ◽  
Binding Sites ◽  
Blood Cells ◽  
Human Lymphocytes ◽  
Immunofluorescent Staining ◽  
Intensity Data ◽  
Blood Samples ◽  
Staining Methods

Monoclonal antibody OKT11 was found to compete with sheep red blood cells for binding sites on human lymphocytes. Preincubation of lymphocytes with OKT11 eliminated E-rosette formation. In a study of 142 peripheral blood samples ranging from 1% to over 90% E-rosette- positive cells, comparison to the percent OKT11-positive cells yielded a correlation coefficient of 0.93. In normal donors, subsets of OKT11+ cells were identified using two-color immunofluorescent staining methods with OKT3, OKT4, and OKT8. On the average, approximately 13% of OKT11+ lymphocytes were OKT3- and 13% of OKT11+ lymphocytes were OKT4- and OKT8-. Based on our double antibody fluorescence intensity data, low antigen density OKT11+ lymphocytes were OKT3-. OKT4+ and OKT8+ lymphocytes in normal peripheral lymphocytes have similar OKT11 antigen density.

scholarly journals Monoclonal antibodies to cholera toxin with special reference to cross-reactions with Escherichia coli heat-labile enterotoxin.

1983 ◽  
Vol 40 (2) ◽  
pp. 570-576 ◽  
Author(s):  
L Lindholm ◽  
J Holmgren ◽  
M Wikström ◽  
U Karlsson ◽  
K Andersson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Special Reference ◽  
Cholera Toxin ◽  
Cross Reactions ◽  
Heat Labile
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