scholarly journals Immunocytochemical Labelling of Haematological Samples Using Monoclonal Antibodies

Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 127
Author(s):  
Wendy N. Erber
Keyword(s):  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
University Of Oxford ◽  
Alkaline Phosphatase Staining ◽  
Nuffield Department ◽  
Staining Methods ◽  
Biological Discovery ◽  
The University ◽  
Productive Time

I reflect on my experience working with David Y. Mason in the Leukaemia Research Laboratories in the Nuffield Department of Pathology at the University of Oxford in the early 1980s. This was soon after the first monoclonal antibodies had been produced, which led to an exciting and productive time in biological discovery and pathology diagnostics. A specific focus in the laboratory was the development of immunoenzymatic staining methods that would enable monoclonal antibodies to be applied in diagnostic practice. This paper describes the work that led to the performance of immuno-alkaline phosphatase staining on blood and bone marrow smears, the success of which changed leukaemia diagnosis.

scholarly journals Double epi-illumination microscopy with separate visualization of two antigens: a combination of epi-polarization for immunogold-silver staining and epi-fluorescence for alkaline phosphatase staining.

1994 ◽  
Vol 42 (3) ◽  
pp. 289-295 ◽  
Author(s):  
C M van der Loos ◽  
A E Becker
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Tissue Section ◽  
Alkaline Phosphatase Activity ◽  
Silver Staining ◽  
Double Staining ◽  
Becton Dickinson ◽  
Photographic Recording ◽  
Alkaline Phosphatase Staining ◽  
Staining Methods

We present a method for an epi-illumination immunohistochemical double staining approach. The method combines the use of an immuno-alkaline phosphatase technique and the immunogold-silver technique, visualized with epifluorescence and epi-polarization illumination, respectively. Out of six tested alkaline phosphatase activity-revealing methods, only the reaction product obtained with the Becton Dickinson CAS Red kit showed an intense red fluorescence with a rhodamine filter set and no signal with epi-polarization illumination. The silver precipitate did not exhibit any signal with the rhodamine filter set. This allows separate observation and photographic recording of two antigens in one tissue section, an objective that cannot be achieved with conventional immunoenzyme double staining methods. The double epi-illumination approach presented is compatible with different immunoenzyme double staining protocols.

scholarly journals Effect of miR-192-5p on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Idiopathic Scoliosis by Regulating Wnt/β-catenin Signaling Pathway via RSPO1

2021 ◽  
Author(s):  
Yifan Yang ◽  
Jing Xu ◽  
Qingxin Su ◽  
Yiran Wu ◽  
Qizheng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Alizarin Red ◽  
Alkaline Phosphatase Staining ◽  
Marrow Mesenchymal Stem Cells ◽  
Related Proteins

Abstract BackgroundIdiopathic scoliosis (IS) is the most common structural scoliosis, which seriously affects not only patient’s physical and mental health but also quality of patient’s life. Abnormal osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is one of the causes of IS. However, the regulation mechanism of osteogenic differentiation of BMSCs in patients with IS remains to be further studied.MethodsSerum samples of 135 patients with IS were collected, and the expression of miRNA were detected by RT-qPCR. BMSCs from patients with IS were collected and the expression of miR-192-5p in BMSCs from IS patients and normal BMSCs was detected by RT-qPCR. Double luciferase reporter genes assay was used to verify the targeting relationship between miR-192-5p and RSPO1. The levels of RSPO1, osteogenic related proteins (OC, OPN and RUNX2) and Wnt/β-catenin signaling pathway related proteins (WNT3A and β-catenin) were detected by Western blotting. Alkaline phosphatase staining and alizarin red staining were used to evaluate the osteogenesis of BMSCs.ResultsmiR-192-5p was significantly up-regulated in serum and BMSCs of patients with IS. Alkaline phosphatase staining and alizarin red staining showed that miR-192-5p inhibitor promoted the osteogenic differentiation of BMSCs from IS patients. miR-192-5p targeted down-regulated the expression of RSPO1 in BMSCs from IS patients. In addition, overexpression of RSPO1 activated Wnt/β-catenin signaling pathway in BMSCs from IS patients. Furthermore, miR-192-5p/RSPO1 axis regulated levels of osteogenic related proteins (OC, OPN and RUNX2) in BMSCs from IS patients through Wnt/β-catenin signaling pathway, and affected the osteogenic differentiation of BMSCs.ConclusionmiR-192-5p, which was highly expressed in patients with IS, inhibited Wnt/β-catenin signaling pathway by down-regulating RSPO1 protein and then reduced the osteogenic differentiation ability of BMSCs.

scholarly journals Two further alkaline phosphatase staining methods for immunohistochemistry.

1984 ◽  
Vol 37 (9) ◽  
pp. 1078-1079 ◽  
Author(s):  
P Fritz ◽  
H V Tuczek ◽  
J G Saal ◽  
G Wegner
Keyword(s):  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Staining ◽  
Staining Methods

Detection of metastatic tumour cells in routine bone marrow smears by immuno-alkaline phosphatase labelling with monoclonal antibodies

1985 ◽  
Vol 61 (1) ◽  
pp. 21-30 ◽  
Author(s):  
A. K. Ghosh ◽  
W. N. Erber ◽  
C. S. R. Hatton ◽  
N. T. J. O'Connor ◽  
B. Falini ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
Tumour Cells ◽  
Metastatic Tumour

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

Combinations of nonlabeled, 125I-Labeled, and Anti-Idiotypic Antiplacental Alkaline Phosphatase Monoclonal Antibodies at Experimental Radioimmunotargeting

1997 ◽  
Vol 38 (6) ◽  
pp. 1087-1093 ◽  
Author(s):  
R. Rossi Norrlund ◽  
D. Holback ◽  
L. Johansson ◽  
S. -O. Hietala ◽  
K. Riklund Åhlstrom
Keyword(s):  
Alkaline Phosphatase ◽  
Monoclonal Antibodies

Matthew Cheung Salisbury, Worship in Medieval England. Past Imperfect Series. Croydon: ARC Humanities Press, 2018, 92 pages.

Mediaevistik ◽  
2020 ◽  
Vol 32 (1) ◽  
pp. 315-318
Author(s):  
Jane Beal
Keyword(s):  
English Translation ◽  
Recent Book ◽  
Medieval England ◽  
Late Medieval ◽  
Cambridge University ◽  
Liturgical Studies ◽  
University Of Oxford ◽  
Medieval Church ◽  
Late Medieval England ◽  
The University

Matthew Cheung Salisbury, a Lecturer in Music at University and Worcester College, Oxford, and a member of the Faculty of Music at the University of Oxford, wrote this book for ARC Humanities Press’s Past Imperfect series (a series comparable to Oxford’s Very Short Introductions). Two of his recent, significant contributions to the field of medieval liturgical studies include The Secular Office in Late-Medieval England (Turnhout: Brepols, 2015) and, as editor and translator, Medieval Latin Liturgy in English Translation (Kalamazoo: Medieval Institute Publications, 2017). In keeping with the work of editors Thomas Heffernan and E. Ann Matter in The Liturgy of the Medieval Church, 2nd ed. (Kalamazoo: Medieval Institute Publications, 2005) and Richard W. Pfaff in The Liturgy of Medieval England: A History (Cambridge University Press, 2009), this most recent book provides a fascinating overview of the liturgy of the medieval church, specifically in England. Salisbury’s expertise is evident on every page.

The Oxford Group and the Emergence of Animal Rights

2020 ◽  
Author(s):  
Robert Garner ◽  
Yewande Okuleye
Keyword(s):  
Animal Rights ◽  
Intellectual Development ◽  
Peter Singer ◽  
Animal Rights Movement ◽  
Friendship Group ◽  
University Of Oxford ◽  
Short Period ◽  
New Ideas ◽  
Oxford Group ◽  
The University

This book is an account of the life and times of a loose friendship group (later christened the Oxford Group) of ten people, primarily postgraduate philosophy students, who attended the University of Oxford for a short period of time from the late 1960s. The Oxford Group, which included—most notably—Peter Singer and Richard Ryder, set about thinking about, talking about, and promoting the idea of animal rights and vegetarianism. The group therefore played a role, largely undocumented and unacknowledged, in the emergence of the animal rights movement and the discipline of animal ethics. Most notably, the group produced an edited collection of articles published as Animals, Men and Morals in 1971 that was instrumental in one of their number—Peter Singer—writing Animal Liberation in 1975, a book that has had an extraordinary influence in the intervening years. The book serves as a case study of how the emergence of important work and the development of new ideas can be explained, and, in particular, how far the intellectual development of individuals is influenced by their participation in a creative community.

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