“Kinetics of molecular transfer across the tegument of protoscoleces and hydatid cysts of Echinococcus granulosus and the relevance of these studies to drug targeting”

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.

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Haplotyping of Echinococcus granulosus Isolates From Human, Sheep and Cattles Hydatid Cysts in Some Central Euphrates Provinces, Iraq

Indian Journal of Forensic Medicine & Toxicology ◽

10.37506/ijfmt.v15i3.15667 ◽

2021 ◽

Keyword(s):

Echinococcus Granulosus ◽

Hydatid Cysts

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Kinetik der Reaktion von 2.4-Dinitrofluorbenzol mit Imidazol-, SH- und Imidazol-SH-Verbindungen / Kinetics of the Reaction of 2,4-dinitro-1-fluorobenzene with Imidazole-, SH- and Imidazole-SH-compounds

Zeitschrift für Naturforschung B ◽

10.1515/znb-1971-1011 ◽

1971 ◽

Vol 26 (10) ◽

pp. 1010-1016 ◽

Cited By ~ 2

Author(s):

Renate Voigt ◽

Helmut Wenck ◽

Friedhelm Schneider

Keyword(s):

Temperature Dependence ◽

Rate Constants ◽

Reaction Rate ◽

Ph Dependence ◽

First Order ◽

Molecular Transfer ◽

Thiolate Anion ◽

Nucleophilic Reactivity ◽

Kinetics Of ◽

Lindley Equation

First order rate constants of the reaction of a series of SH-, imidazole- and imidazole/SH-compounds with FDNB as well as their pH- and temperature dependence were determined. Some of the tested imidazole/SH-compounds exhibit a higher nucleophilic reactivity as is expected on the basis of their pKSH-values. This enhanced reactivity is caused by an activation of the SH-groups by a neighbouring imidazole residue. The pH-independent rate constants were calculated using the Lindley equation.The kinetics of DNP-transfer from DNP-imidazole to SH-compounds were investigated. The pH-dependence of the reaction displays a maximum curve. Donor in this reaction is the DNP-imidazolecation and acceptor the thiolate anion.The reaction rate of FDNB with imidazole derivatives is two to three orders of magnitude slower than with SH-compounds.No inter- or intra-molecular transfer of the DNP-residue from sulfure to imidazole takes place.

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Genetic Characterization of Human-Derived Hydatid Cysts of Echinococcus granulosus Sensu Lato in Heilongjiang Province and the First Report of G7 Genotype of E. canadensis in Humans in China

PLoS ONE ◽

10.1371/journal.pone.0109059 ◽

2014 ◽

Vol 9 (10) ◽

pp. e109059 ◽

Cited By ~ 16

Author(s):

Tiemin Zhang ◽

Dong Yang ◽

Zhaolin Zeng ◽

Wei Zhao ◽

Aiqin Liu ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Genetic Characterization ◽

Heilongjiang Province ◽

Hydatid Cysts ◽

First Report ◽

Echinococcus Granulosus Sensu Lato

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Echinococcus granulosus: Pre-culture of protoscoleces in vitro significantly increases development and viability of secondary hydatid cysts in mice

Experimental Parasitology ◽

10.1016/j.exppara.2005.02.003 ◽

2005 ◽

Vol 110 (1) ◽

pp. 88-90 ◽

Cited By ~ 24

Author(s):

Wen-Bao Zhang ◽

Malcolm K. Jones ◽

Jun Li ◽

Donald P. McManus

Keyword(s):

Echinococcus Granulosus ◽

Hydatid Cysts

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Observations on the suitability and importance of the domestic intermediate hosts of Echinococcus granulosus in Uttah Pradesh, India

Journal of Helminthology ◽

10.1017/s0022149x00008701 ◽

1989 ◽

Vol 63 (1) ◽

pp. 39-45 ◽

Cited By ~ 23

Author(s):

M. Irshadullah ◽

W. A. Nizami ◽

C. N. L. Macpherson

Keyword(s):

Life Cycle ◽

Intermediate Host ◽

Echinococcus Granulosus ◽

Frequency Distribution ◽

Host Species ◽

Control Measures ◽

The Other ◽

Hydatid Cysts ◽

Intermediate Hosts ◽

Cyst Size

ABSTRACTThe present study investigated the suitability and importance of buffaloes, camels, sheep, goats and pigs in maintaining the life-cycle of Echinococcus granulosus in Aligarh, India. A total of 565 (36%) of 1556 buffaloes, 20 (2%) of 1208 goats, 5 (1%) of 559 pigs, 6 (6%) of 109 sheep and two of three camels were found to harbour hydatid cysts. The frequency distribution of the hydatid cysts in each intermediate host species was over-dispersed and in buffaloes cyst fertility increased with increasing cyst size. Of 2171, 95 and four buffalo, goat, and camel cysts examined 327 (15%), two (2%) and three cysts respectively were fertile. No pig or sheep cysts were found to contain protoscoleces. The unfenced buffalo abattoir and the large number of dogs allowed access to the abattoir coupled to the number of buffaloes slaughtered in comparison to the other potential hosts, indicates that the buffalo is the most significant host for maintaining the life-cycle of the parasite in this area of India. Applicable control measures for the region are suggested.

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Metacestodes of moose, Alces alces, of the Chapleau Crown Game Preserve, Ontario

Canadian Journal of Zoology ◽

10.1139/z79-212 ◽

1979 ◽

Vol 57 (8) ◽

pp. 1619-1623 ◽

Cited By ~ 4

Author(s):

E. M. Addison ◽

A. Fyvie ◽

F. J. Johnson

Keyword(s):

Echinococcus Granulosus ◽

Lung Tissue ◽

Physical Condition ◽

Alces Alces ◽

Age Groups ◽

Hydatid Cysts ◽

Intensity Of Infection ◽

Taenia Hydatigena ◽

Apparent Relationship

Lungs, liver, spleen, heart, and kidneys of moose, Alces alces, collected from 1963 to 1965 in the Chapleau Crown Game Preserve of northeastern Ontario were examined for parasites. Thirty-eight of 51 moose (75%) were infected with metacestodes of Taenia hydatigena Pallas, 1766; 40 of 54 (74%) with Taenia krabbei Moniez, 1879; and 36 of 54 (67%) with Echinococcus granulosus (Batsch, 1786). Twenty-two of 51 (43%) moose harboured all three species. Each species was more prevalent in older moose than in young moose and intensity of infections increased with the age of moose. Occurrence of small hydatid cysts decreased and large cysts increased with increasing age of moose. Of 1154 hydatid cysts, 95.3% were in lung tissue, 3.6% in liver, 0.9% in spleen, and 1 cyst (0.1%) was recovered from each of heart and kidney. Degenerate cysticerci of T. hydatigena and T. krabbei were observed in all age groups of moose. There was no apparent relationship between intensity of infection with metacestodes and physical condition of moose.

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Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina

Parasitology Research ◽

10.1007/s00436-017-5555-2 ◽

2017 ◽

Vol 116 (9) ◽

pp. 2599-2604 ◽

Cited By ~ 4

Author(s):

María Florencia Debiaggi ◽

Silvia Viviana Soriano ◽

Nora Beatriz Pierangeli ◽

Lorena Evelina Lazzarini ◽

Luis Alfredo Pianciola ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Genetic Characterization ◽

Sensu Stricto ◽

Hydatid Cysts ◽

Echinococcus Canadensis ◽

Echinococcus Granulosus Sensu Stricto

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Canine echinococcosis: the predominance of immature eggs in adult tapeworms of Echinococcus granulosus in stray dogs from Tunisia

Journal of Helminthology ◽

10.1017/s0022149x16000341 ◽

2016 ◽

Vol 91 (3) ◽

pp. 380-383 ◽

Cited By ~ 1

Author(s):

W. Iraqi

Keyword(s):

Public Health ◽

Echinococcus Granulosus ◽

Hydatid Disease ◽

Health Problem ◽

Small Proportion ◽

Public Health Problem ◽

Hydatid Cysts ◽

Intermediate Hosts ◽

Stray Dogs ◽

Adult Tapeworm

AbstractCanine echinococcosis is caused by the adult tapeworm of Echinococcus granulosus. As intermediate hosts, humans and livestock become infected following ingestion of eggs that are passed in the faeces of dogs. Mature eggs develop into hydatid cysts in different organs, leading to hydatid disease, which is a serious public health problem. In the present study, we investigated the proportion of mature eggs of E. granulosus in 140 dogs from three regions of Tunisia. The results showed the predominance of immature E. granulosus eggs in infected dogs and the occurrence of a small proportion of oncospheres. The ability of immature eggs to infect humans and livestock is discussed.

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