molecular transfer
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2022 ◽  
Vol 9 (1) ◽  
pp. 18
Author(s):  
Aleksandr G. Novoselov ◽  
Sergei A. Sorokin ◽  
Igor V. Baranov ◽  
Nikita V. Martyushev ◽  
Olga N. Rumiantceva ◽  
...  

This article puts forward arguments in favor of the necessity of conducting complex measurements of molecular transport coefficients that quantitatively determine the coefficients of dynamic viscosity, thermal diffusivity and molecular diffusion. The rheological studies have been carried out on the viscometers of two types: those with a rolling ball (HÖPPLER® KF 3.2.), and those with a rotary one (Rheotest RN 4.1.). The thermophysical studies have been performed using the analyzer Hot Disk TPS 2500S. The measurements have been taken in the temperature range of 283 to 363 K. The concentration of dry substances has varied from 16.2 to 77.7% dry wt. An empirical equation for calculating the density of aqueous solutions of beet molasses has been obtained. The diagrams of the dependence of the dynamic viscosity on the shear rate in the range of 1 s−1 to 500 s−1 at different temperatures have been provided. The diagrams of the dependence of the coefficients of thermal conductivity and thermal diffusivity on the temperature and the concentration of dry substances have been presented, and empirical equations for their calculation have been obtained. The findings can be used for engineering calculations of hydrodynamic and heat-exchange processes in biotechnological equipment.


2021 ◽  
Vol 17 (12) ◽  
pp. 2271-2297
Author(s):  
Mengxi Zhu ◽  
Shan Li ◽  
Sanqiang Li ◽  
Haojie Wang ◽  
Juanjuan Xu ◽  
...  

Exosomes are representative of a promising vehicle for delivery of biomolecules. Despite their discovery nearly 40 years, knowledge of exosomes and extracellular vesicles (EVs) and the role they play in etiology of disease and normal cellular physiology remains in its infancy. EVs are produced in almost all cells, containing nucleic acids, lipids, and proteins delivered from donor cells to recipient cells. Consequently, they act as mediators of intercellular communication and molecular transfer. Recent studies have shown that, exosomes are associated with numerous physiological and pathological processes as a small subset of EVs, and they play a significant role in disease progression and treatment. In this review, we discuss several key questions: what are exosomes, why do they matter, and how do we repurpose them in their strategies and applications in drug delivery systems. In addition, opportunities and challenges of exosome-based theranostics are also described and directions for future research are presented.


2021 ◽  
Vol 2100 (1) ◽  
pp. 012024
Author(s):  
B M Burakhanov

Abstract The very fact of molecular transfer of the amount of motion, including in an ideal gas in an equilibrium state, has long been well known. However, this fact is still not realized as a physical phenomenon of transfer, equivalent to such transfer phenomena as diffusion, thermal conductivity and viscosity. The key concept used in this paper when describing the phenomenon of relay race molecular transfer of the amount of motion is the concept - “molecular relay race type of motion”. A molecular relay race model of an ideal gas in an equilibrium state is proposed, as well as a molecular relay race model of a mixture of two ideal gases at constant temperature and pressure. It is shown that the value of the velocity modulus of diffusion flows is one of the physical characteristics of the mixture as a whole. It is also shown that the total density of the substance carried by the diffusion flows is many orders of magnitude less than the total density of the inhomogeneous multicomponent mixture.


Author(s):  
A. I. Kitsak

The relevance of the work is due to the lack of a physical interpretation of the process of extinguishing jet burning systems with fire extinguishing powders, which is important for ensuring effective fire extinguishing at gas and oil complexes and hazardous chemical industries. A mathematical model of the reaction kinetics of heterogeneous inhibition of active flame centers of a jet burning system by fire extinguishing powder particles in an unsteady mode is considered in the approximation of a purely molecular transfer of matter in the reaction zone. The regularities of the mechanism of heterogeneous inhibition of the active flame centers by the particles of the extinguishing powder under conditions when the active particles of the combustion products participate not only in diffuse, but also in convective transport are established. It is shown, that the convective motion of the active flame centers increases the reaction rate of heterogeneous inhibition of their particles of the extinguishing agent. The results obtained allow us to optimize the conditions for the supply of fire extinguishing powder to the jet burning medium for effective flame suppression.


2021 ◽  
Author(s):  
Meng Qin ◽  
Zhenxing Liu ◽  
Wei Wang ◽  
D. Thirumalai

We use simulations based on an all atom Go model to calculate the folding temperatures (Tfs) and free energies (ΔGs) of two variants of the WW domain, which is a small all β-sheet protein. The results, without adjusting any parameter, are in good agreement with experiments, thus validating the simulations. We then used the Molecular Transfer Model to predict the changes in their ΔG and Tfs as Guanidine Hydrochloride concentration is varied. The predictions can be readily tested in experiments.


Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1328
Author(s):  
Yanyin Lu ◽  
Takanori Eguchi ◽  
Chiharu Sogawa ◽  
Eman A. Taha ◽  
Manh Tien Tran ◽  
...  

Extracellular vesicles (EV) heterogeneity is a crucial issue in biology and medicine. In addition, tumor-associated macrophages are key components in cancer microenvironment and immunology. We developed a combination method of size exclusion chromatography and concentration filters (SEC-CF) and aimed to characterize different EV types by their size, cargo types, and functions. A human monocytic leukemia cell line THP-1 was differentiated to CD14-positive macrophage-like cells by stimulation with PMA (phorbol 12-myristate 13-acetate) but not M1 or M2 types. Using the SEC-CF method, the following five EV types were fractionated from the culture supernatant of macrophage-like cells: (i) rare large EVs (500–3000 nm) reminiscent of apoptosomes, (ii) EVs (100–500 nm) reminiscent of microvesicles (or microparticles), (iii) EVs (80–300 nm) containing CD9-positive large exosomes (EXO-L), (iv) EVs (20–200 nm) containing unidentified vesicles/particles, and (v) EVs (10–70 nm) containing CD63/HSP90-positive small exosomes (EXO-S) and particles. For a molecular transfer assay, we developed a THP-1-based stable cell line producinga GFP-fused palmitoylation signal (palmGFP) associated with the membrane. The THP1/palmGFP cells were differentiated into macrophages producing palmGFP-contained EVs. The macrophage/palmGFP-secreted EXO-S and EXO-L efficiently transferred the palmGFP to receiver human oral carcinoma cells (HSC-3/palmTomato), as compared to other EV types. In addition, the macrophage-secreted EXO-S and EXO-L significantly reduced the cell viability (ATP content) in oral carcinoma cells. Taken together, the SEC-CF method is useful for the purification of large and small exosomes with higher molecular transfer activities, enabling efficient molecular delivery to target cells.


2021 ◽  
Vol 258 ◽  
pp. 08020
Author(s):  
Mukhiddin Khudjaev ◽  
Erkin Nematov ◽  
Anorgul Karimova ◽  
Doston Khurramov ◽  
Botir Khasanov

The article deals with modeling the process of force load generation at an initial periodic change in pressure (a plane problem). The subject of research is a pulsating flow in a flat channel at an initial periodic pressure change. The determination of flow parameters with a periodic change in the inlet pressure; the changes in the structure of the working fluid associated with the release of various particles from the pipe walls, the addition of impurities to prevent leaks, and the high-speed modes, are given in the article considering the law of molecular and molar transfer between layers. Research methods are based on Newton’s rheological law, according to which molecular transfer is described by the law of proportionality of stresses to the derivative of the normal velocity; on the method of accounting for molar transfer by proportionality of stresses to the derivative of normal acceleration; on the method of mathematical modeling and the analytical method for their solutions, based on the provisions of operational calculus. An analytical solution to the problem of pulsating fluid motion in a plane-parallel channel is obtained with allowance for single and group transfer of molecules in the flow. The application of the analytical expressions obtained for the velocities is not limited to the critical Reynolds number, i.e. they are applied for any values of this number. Analytical expressions are obtained for the transverse and longitudinal components of the flow velocity. The resulting solution describes two zones of flow: in the first zone, two types of transfer occur, depending on the flow pattern, either molecular or molar transfer of fluid volumes between the layers prevails. In the second zone, only molecular transfer occurs.


2020 ◽  
Author(s):  
Mauro L. Mugnai ◽  
D. Thirumalai

AbstractWe present a theoretical method to study how changes in pH shape the heterogeneous conformational ensemble explored by intrinsically disordered proteins (IDPs). The theory is developed in the context of coarse-grained models, which enable a fast, accurate, and extensive exploration of conformational space at a given protonation state. In order to account for pH effects, we generalize the Molecular Transfer Model (MTM), in which conformations are re-weighted using the transfer free energy, which is the free energy necessary for bringing to equilibrium in a new environment a “frozen” conformation of the system. Using the semi-grand ensemble, we derive an exact expression of the transfer free energy, which amounts to the appropriate summation over all the protonation states. Because the exact result is computationally too demanding to be useful for large polyelectrolytes or IDPs, we introduce a mean-field (MF) approximation of the transfer free energy. Using a lattice model, we compare the exact and MF results for the transfer free energy and a variety of observables associated with the model IDP. We find that the precise location of the charged groups (the sequence), and not merely the net charge, determines the structural properties. We demonstrate that some of the limitations previously noted for MF theory in the context of globular proteins are mitigated when disordered polymers are studied. The excellent agreement between the exact and MF results poises us to use the method presented here as a computational tool to study the properties of IDPs and other biological systems as a function of pH.


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