Raman and FT-IR Spectroscopy of Drug-Biological Target Interactions In Vitro and In Vivo

1984 ◽  
pp. 113-136 ◽  
Author(s):  
M. Manfait ◽  
T. Theophanides ◽  
A. J. P. Alix ◽  
P. Jeannesson
Keyword(s):  
Ir Spectroscopy ◽  
Biological Target ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Contribution of ATR/FT-IR Spectroscopy for Studying the in vivo Behavior of Octylmethoxycinnamate (OMC) after Topical Application

2001 ◽  
Vol 55 (9) ◽  
pp. 1173-1180 ◽  
Author(s):  
C. Laugel ◽  
C. Do Nascimento ◽  
D. Ferrier ◽  
J. P. Marty ◽  
A. Baillet
Keyword(s):  
Ir Spectroscopy ◽  
Topical Application ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Fourier-transform infrared spectroscopy: a pharmacotoxicologic tool for in vivo monitoring radical aggression

2001 ◽  
Vol 79 (2) ◽  
pp. 158-165 ◽  
Author(s):  
Anne-Marie Melin ◽  
Annie Perromat ◽  
Gérard Deleris
Keyword(s):  
Fourier Transform ◽  
Carbon Tetrachloride ◽  
Ir Spectroscopy ◽  
Liver Microsomes ◽  
Fourier Transform Infrared ◽  
Conformational Structure ◽  
Ft Ir ◽  
The Brain ◽  
Ft Ir Spectroscopy

Among the physico-chemical methods that can be used to investigate induced peroxidation in living cells, Fourier transform infrared (FT-IR) spectroscopy appears to be a valuable technique as it is non-destructive and sensitive for monitoring changes in the vibrational spectra of samples. We examined microsomal fractions from rat liver and brain by FT-IR to study the effect of radical aggression induced in vivo by carbon tetrachloride (CCl4). The length of the acyl chains was increased as a consequence of peroxidation induced by the xenobiotic. Moreover, an enhanced level of cholesterol esters and an increase in phospholipids were observed in the liver and the brain, respectively. The conformational structure of the membrane proteins was changed in both the liver and the brain. In the polysaccharide region, we observed an important loss in glucidic structures, such as a decrease in liver glycogen and in some brain glycolipids. These alterations are probably due to the interactions between cells and CCl4and the metabolic changes caused by CCl4. Thus, FT-IR spectroscopy appears to be an useful tool and an accurate means for rapidly investigating the in vivo biochemical alterations induced by CCl4in microsomes, and for correlating them with biochemical and physiological data.Key words: brain, carbon tetrachloride, FT-IR, liver, microsomes.

scholarly journals FT-IR Spectroscopy Based Evaluation of Changes in Primary Metabolites of Amsonia orientalis after In vitro 6-benzylaminopurine Treatment

2016 ◽  
Vol 44 (1) ◽  
pp. 209-214 ◽  
Author(s):  
Arda ACEMİ ◽  
Sevgi TÜRKER-KAYA ◽  
Fazıl ÖZEN
Keyword(s):  
Ir Spectroscopy ◽  
Plant Growth ◽  
Natural Populations ◽  
Molecular Data ◽  
Primary Metabolites ◽  
Bern Convention ◽  
Ft Ir ◽  
Common Plant ◽  
Ft Ir Spectroscopy

Amsonia orientalis Decne. (syn. Rhazya orientalis (Decne.) A. DC.) is only cultivated as an ornamental plant but also has a medicinal merit. The natural populations of the species were taken under conservation as per the Bern Convention by European Council. As one of the most common plant growth regulators and synthetic agricultural fertilizers, the effects of 6-benzylaminopurine (BAP) at different concentrations on primary metabolites of in vitro propagated A. orientalis have been investigated by using Fourier transform infrared (FT-IR) spectroscopy. Among all applied concentrations, 2.0 mg l-1 BAP resulted in increment of hydrogen bonded polysaccharide and proteins concentrations, lipid structure and membrane fluidity. Treatments of 2.0 and 4.0 mg l-1 BAP increased the existence of ferulic acid which also indicates the linkage of xylan and both cellulose and hemicellulose content. There was a significant decrease in the area of the peak which indicated the C=O stretching of proteins and pectins for 0.5 and 1.0 mg l-1 BAP while there was a significant increase for 2.0 and 4.0 mg l-1 of BAP treatments. The evaluation of molecular data from FT-IR spectroscopy as in the current study might help to estimate different physiological changes at the molecular level in plants depending on plant growth regulator and/or fertilizer applications. Thus, this FT-IR based method can be applied to in vitro propagation studies and also to studies focused on effects of pesticide and/or herbicide applications on plants to understand the metabolic changes in target species.

The in vivo toxicity of carbon tetrachloride and carrageenan on heart microsomes: analysis by Fourier transform infrared spectroscopy

2001 ◽  
Vol 79 (9) ◽  
pp. 799-804 ◽  
Author(s):  
Anne-Marie Melin ◽  
Annie Perromat ◽  
Gérard Deleris
Keyword(s):  
Fourier Transform ◽  
Carbon Tetrachloride ◽  
Ir Spectroscopy ◽  
Rat Heart ◽  
Great Part ◽  
Total Lipid Content ◽  
Fourier Transform Infrared ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

We investigated the sensitivity of rat heart microsomes to free radical attack using Fourier transform infrared (FT-IR) spectroscopy. This physico-chemical method seemed a valuable technique: quite sensitive to changes in the vibrational spectra. The spectral variations observed between normal and treated rats were in great part due to reactive oxygen species that led to changes in protein conformation involving β-sheets, aggregation of proteins, and modification of protein synthesis. Carrageenan-induced inflammation slightly enhanced the total lipid content; rearrangement of acyl chains and accumulation of cholesterol esters and phospholipids also occurred in the treated rats. Carbon tetrachloride induced a decrease in both lipid and protein contents. The level of glucidic substrates was diminished with carbon tetrachloride and enhanced with carrageenan; these changes were due to metabolic interactions between cell components and drugs. FT-IR spectroscopy provided an accurate means to monitor, in rat heart, the in vivo effects of inflammatory and peroxidative damages, to discriminate and classify the affected cells, and to correlate the findings with known physiological and biochemical data in close relationship with metabolic disruptions induced by the two xenobiotics. Key words: heart microsomes, carbon tetrachloride, carrageenan, peroxidation, FT-IR.

scholarly journals Curcuminoid Co-Loading Platinum Heparin-Poloxamer P403 Nanogel Increasing Effectiveness in Antitumor Activity

Gels ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 59
Author(s):  
Ngoc The Nguyen ◽  
Quynh Anh Bui ◽  
Hoang Huong Nhu Nguyen ◽  
Tien Thanh Nguyen ◽  
Khanh Linh Ly ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Synergistic Effects ◽  
Effective Delivery ◽  
Delivery Platform ◽  
Ft Ir ◽  
High Antitumor Activity ◽  
Dual Drug ◽  
Ft Ir Spectroscopy

Nanosized multi-drug delivery systems provide synergistic effects between drugs and bioactive compounds, resulting in increased overall efficiency and restricted side effects compared to conventional single-drug chemotherapy. In this study, we develop an amphiphilic heparin-poloxamer P403 (HP403) nanogel that could effectively co-load curcuminoid (Cur) and cisplatin hydrate (CisOH) (HP403@CisOH@Cur) via two loading mechanisms. The HP403 nanogels and HP403@CisOH@Cur nanogels were closely analyzed with 1H-NMR spectroscopy, FT-IR spectroscopy, TEM, and DLS, exhibiting high stability in spherical forms. In drug release profiles, accelerated behavior of Cur and CisOH at pH 5.5 compared with neutral pH was observed, suggesting effective delivery of the compounds in tumor sites. In vitro studies showed high antitumor activity of HP403@CisOH@Cur nanogels, while in vivo assays showed that the dual-drug platform prolonged the survival time of mice and prevented tail necrosis. In summary, HP403@CisOH@Cur offers an intriguing strategy to achieve the cisplatin and curcumin synergistic effect in a well-designed delivery platform that increases antitumor effectiveness and overcomes undesired consequences caused by cisplatin in breast cancer treatment.

scholarly journals 99mTc Labelling Strategies for the Development of Potential Nitroimidazolic Hypoxia Imaging Agents

Inorganics ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 128 ◽  
Author(s):  
Giglio ◽  
Rey
Keyword(s):  
Rational Design ◽  
Biological Properties ◽  
Fine Tuning ◽  
Oxidation States ◽  
Hypoxia Imaging ◽  
Biological Target ◽  
99Mtc Radiopharmaceuticals ◽  
99Mtc Labelling

Technetium-99m has a rich coordination chemistry that offers many possibilities in terms of oxidation states and donor atom sets. Modifications in the structure of the technetium complexes could be very useful for fine tuning the physicochemical and biological properties of potential 99mTc radiopharmaceuticals. However, systematic study of the influence of the labelling strategy on the “in vitro” and “in vivo” behaviour is necessary for a rational design of radiopharmaceuticals. Herein we present a review of the influence of the Tc complexes’ molecular structure on the biodistribution and the interaction with the biological target of potential nitroimidazolic hypoxia imaging radiopharmaceuticals presented in the literature from 2010 to the present. Comparison with the gold standard [18F]Fluoromisonidazole (FMISO) is also presented.

Sign in / Sign up

Export Citation Format

Share Document