Effects of a Beta-Adrenergic Agonist (Reproterol) on Ciliary Beat Frequency of Human Bronchial Epithelium in Vitro

1981 ◽  
pp. 485-485
Author(s):  
R. Kasparek ◽  
W. Mizera ◽  
H. Hesse
Keyword(s):  
Beat Frequency ◽  
Bronchial Epithelium ◽  
Ciliary Beat Frequency ◽  
Adrenergic Agonist ◽  
Beta Adrenergic ◽  
Beta Adrenergic Agonist ◽  
Ciliary Beat

Regulation of ciliary beat frequency by autonomic mechanisms: in vitro

1988 ◽  
Vol 65 (4) ◽  
pp. 1895-1901 ◽  
Author(s):  
L. B. Wong ◽  
I. F. Miller ◽  
D. B. Yeates
Keyword(s):  
Laser Light ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Atropine Sulfate ◽  
Adrenergic Agonist ◽  
Muscarinic Cholinergic ◽  
Autonomic Receptors ◽  
Stimulation Of ◽  
Ciliary Beat

The ciliated epithelium of the mammalian trachea separates the neurohumoral milieu of the tissue from that of the environment of the airway lumen. To determine whether specific autonomic receptors regulating ciliary beat frequency (CBF) were located on mucosal or serosal sides, we measured CBF by heterodyne mode correlation analysis laser light scattering in bovine tracheal tissues mounted in a two-sided chamber. A beta 2-adrenergic agonist, fenoterol, at 10(-7) M, stimulated serosal CBF from 7.9 +/- 1.3 to 20.2 +/- 5.8 Hz (P less than 0.01) and mucosal CBF from 6.6 +/- 0.9 to 14.7 +/- 4.6 Hz (P less than 0.01). A muscarinic cholinergic agonist, methacholine, at 10(-7) M, increased mucosal CBF from 8.4 +/- 1.0 to 19.5 +/- 5.5 Hz (P less than 0.01) and serosal CBF from 8.0 +/- 0.9 to 15.4 +/- 5.0 Hz (P less than 0.01). The differences in stimulation of CBF on the mucosal and serosal sides between fenoterol and methacholine were significant (P less than 0.01). Studies in which these autonomic agonist stimulating effects were inhibited by their respective antagonists, propranolol and atropine sulfate, demonstrated that CBF can be regulated independently by mediators both in the submucosa and within the mucus lining.

The effects of in vitro cotitine on nasal ciliary beat frequency

1995 ◽  
Vol 20 (5) ◽  
pp. 465-469 ◽  
Author(s):  
A. M. AGIUS ◽  
M. WAKE ◽  
A. L. PAHOR ◽  
A. SMALLMAN
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Ciliary Beat

Effects of ethanol on in vitro ciliary motility

1988 ◽  
Vol 65 (4) ◽  
pp. 1617-1620 ◽  
Author(s):  
D. R. Maurer ◽  
J. Liebman
Keyword(s):  
Beat Frequency ◽  
Slow Motion ◽  
Ciliary Beat Frequency ◽  
Blood Levels ◽  
Ciliary Beating ◽  
Ciliary Motility ◽  
Social Drinking ◽  
High Concentrations ◽  
Ciliary Beat

Consumption of ethanol can impair lung function and slow total lung clearance. High concentrations of ethanol have been shown to slow or arrest ciliary beating. This study examined the effects of concentrations of alcohol comparable to blood levels achieved from social drinking on ciliary beat frequency. We obtained ciliated cells by brushing the trachea of unanesthetized sheep during fiber-optic bronchoscopy. The cells were suspended in a perfusion chamber and physiological conditions were maintained in vitro. Ciliary beat frequency and synchrony were determined by slow-motion analysis of video images obtained by interference contrast microscopy. Metachronal ciliary coordination was observed in all preparations. The ciliary beat frequency was stimulated at ethanol concentrations from 0.01 up to but not including 0.1%, unchanged at 0.5 and 1%, and slowed at 2%. While confirming inhibition of ciliary motility at very high ethanol levels, we observed no acute impairment of ciliary function at ethanol concentrations comparable to those achieved from social drinking. Indeed, we found an unexpected stimulation of ciliary beating at low levels of ethanol. How this alteration in ciliary beating would affect pulmonary clearance remains unknown at this time.

The in Vivo and in Vitro Effect of Phenylephrine (Neo Synephrine) on Nasal Ciliary Beat Frequency and Mucociliary Transport

1990 ◽  
Vol 103 (4) ◽  
pp. 558-565 ◽  
Author(s):  
P. Perry Phillips ◽  
Thomas V. McCaffrey ◽  
Eugene B. Kern
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Mucociliary Transport ◽  
Vitro Effect ◽  
Ciliary Beat

Influence of MP 29-02 on ciliary beat frequency in human epithelial cells in vitro

2018 ◽  
Vol 275 (6) ◽  
pp. 1483-1490
Author(s):  
D. Häussler ◽  
J. U. Sommer ◽  
A. Nastev ◽  
C. Aderhold ◽  
A. Wenzel ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Human Epithelial Cells ◽  
Ciliary Beat

Polyhexanide-containing solution reduces ciliary beat frequency of human nasal epithelial cells in vitro

2014 ◽  
Vol 272 (2) ◽  
pp. 377-383 ◽  
Author(s):  
Richard Birk ◽  
C. Aderhold ◽  
J. Stern-Sträter ◽  
K. Hörmann ◽  
B. A. Stuck ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Nasal Epithelial Cells ◽  
Human Nasal Epithelial Cells ◽  
Ciliary Beat

Leukotrienes C4 and D4 Increase the Ciliary Beat Frequency in Human Upper Airway Mucosa in Vitro

1998 ◽  
Vol 118 (4) ◽  
pp. 472-477 ◽  
Author(s):  
Carlos B. Cyrus ◽  
Bin Yang ◽  
Thomas V. McCaffrey
Keyword(s):  
Beat Frequency ◽  
Upper Airway ◽  
Ciliary Beat Frequency ◽  
Airway Mucosa ◽  
Contrast Microscopy ◽  
Leukotriene Receptor ◽  
Upper Respiratory ◽  
In Vitro Effects ◽  
Ciliary Beat

It has been suggested that leukotrienes C4 (LTC4) and D4 (LTD4) released from upper respiratory mucosa influence mucociliary transport during allergic reactions. We studied the in vitro effects of leukotrienes C4 and D4 on the ciliary beat frequency (CBF) of human adenoid explants over a 5-hour period. Tissue explants were cultured at 35° C in Minimum Essential Medium Eagle (MEM). The CBF was measured using phase contrast microscopy and microphotometry. Measurements of CBF were recorded in medium alone and in medium containing LTC4 or LTD4 at concentrations of 10−8 and 10−6 M. LTC4 and LTD4 increased CBF at concentrations of 10−8 and 10−6 M with increases of 20.51% ± 2.69% and 29.84% ± 4.06%, respectively. To determine the specificity of the LTC4 and LTD4 effects, the ciliated epithelium was treated with the specific leukotriene receptor antagonist LY-171,883 before administration of LTC4 and LTD4. LY-171,883 (10−6 M) significantly inhibited the ciliostimulatory effects of both leukotrienes. Our findings indicate that LTC4 and LTD4 increase CBF in vitro by activation of the LTD4 receptor.

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