Effect of Gibberellic Acid (GA3) and Micropropagation on Axillary Shoot Induction in Monostem Genotype (To-2) of Tomato (L. Esculentum Mill)

1995 ◽  
pp. 411-416
Author(s):  
P. Bima ◽  
F. Mensurati ◽  
G. P. Soressi
Keyword(s):  
Gibberellic Acid ◽  
Axillary Shoot ◽  
Shoot Induction

scholarly journals Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

2009 ◽  
Vol 21 (2) ◽  
pp. 43-52 ◽  
Author(s):  
Wojciech Litwińczuk ◽  
Ewa Okołotkiewicz ◽  
Iwona Matyaszek
Keyword(s):  
Gibberellic Acid ◽  
Adventitious Shoots ◽  
Shoot Culture ◽  
Shoot Elongation ◽  
Axillary Shoot ◽  
Shoot Cultures ◽  
Axillary Shoots ◽  
High Doses ◽  
Culture Growth

Abstract In this study, the influence of gibberellic acid (GA3) on strawberry in vitro shoot culture growth and development was investigated. ‘Senga Sengana’ and ‘Elsanta’ clones were grown on the medium recommended by Boxus (1999), supplemented with BA (0.5 mg dm-3), IBA (0.1 mg dm-3), glucose (40.0 g dm-3) and GA3 (0.1 - control, 1.0, 2.0, 5.0, 10.0 mg dm-3). In general, gibberellic acid improved axillary shoot elongation and reduced the growth of callus as well as the formation of roots and the development of adventitious shoots. GA3 applied at a concentration of 1.0-2.0 mg dm-3 significantly increased the number of axillary shoots (mainly crown shoots), whereas under higher (5.0-10.0 mg dm-3) doses it stimulated the development of runners. It seems that a new method of strawberry micropropagation based on the multiplication of axillary crowns and runners, which could also reduce the risk of domination of in vitro cultures with adventitious shoots, might be elaborated

scholarly journals Morphological and Biochemical Responses to Gibberellic Acid in Magnolia × ‘Spectrum’ in Vitro

2016 ◽  
Vol 58 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Agnieszka Wojtania ◽  
Edyta Skrzypek ◽  
Eleonora Gabryszewska
Keyword(s):  
Gibberellic Acid ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Shoot Formation ◽  
Antioxidant Enzyme Activities ◽  
Axillary Shoot ◽  
Soluble Sugar Content ◽  
Total Soluble Sugar ◽  
Leaf Formation

AbstractThe total soluble sugar content and antioxidant enzyme activities were studied for the first time during axillary shoot formation inMagnolia× ‘Spectrum’ in vitro in response to BAP (0.3 mg l−1), different levels of gibberellic acid (GA3; 0.0, 0.1, 0.5, 1.0 mg l−1), sucrose (20 and 30 g l−1) and nitrogen salts (KNO3/NH4NO3; 100/100% and 75/50% relative to MS medium). Among various GA3and sucrose/nitrogen salts ratios, the most effective axillary multiplication (5.9 shoots/explant) and leaf formation (25.7 leaves per multiplied clumps) were obtained after addition of GA3at 0.1 mg l−1to a BAP medium containing 20 g l−1sucrose and reduced levels of nitrogen salts (75% KNO3and 50% NH4NO3). The addition of GA3to the BAP medium enhanced shoot formation by 36% and leaf formation by 27%. The highest shoot formation capacity ofM.× ‘Spectrum’ in vitro coincided with enhanced levels of soluble sugar and peroxidase (POD) activity. Increasing GA3concentration from 0.1 to 1.0 mg l−1in the above medium resulted in inhibition of shoot and leaf formation and a decrease in the soluble sugar content. The influence of GA3on the activities of catalase (CAT) and POD depended on its concentration and the levels of sucrose and nitrogen salts in the medium. The highest increase in CAT and POD activities, that coincided with the enhanced shoot formation capacity ofM.× ‘Spectrum’ in vitro, was observed after addition of GA3to the medium containing high levels of sucrose and nitrogen salts.

scholarly journals Shoot Induction from Axillary Shoot Tip Explants of Fig (Ficus carica) cv. Japanese BTM 6

2018 ◽  
Vol 29 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Wan Ting Ling ◽  
◽  
Fui Chu Liew ◽  
Wei Yong Lim ◽  
Sreeramanan Subramaniam ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Ficus Carica ◽  
Axillary Shoot ◽  
Shoot Induction ◽  
Shoot Tip Explants

Plastid development in germinating wheat (Triticum aestivum) is enhanced by gibberellic acid and delayed by gabaculine

1995 ◽  
Vol 95 (3) ◽  
pp. 336-346 ◽  
Author(s):  
Suhaila Younis ◽  
Margareta Ryberg ◽  
Christer Sundqvist
Keyword(s):  
Triticum Aestivum ◽  
Gibberellic Acid ◽  
Plastid Development

Effects of Defoliation and Foliar Application of Gibberellic Acid on Fruit Softening after Alcohol Treatment of Japanese Persimmon 'Hiratanenashi'.

1994 ◽  
Vol 62 (4) ◽  
pp. 867-875 ◽  
Author(s):  
Hiroyuki Itamura ◽  
Tadaaki Fukushima ◽  
Toshio Kitamura ◽  
Hisashi Harada ◽  
Satoshi Taira ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Foliar Application ◽  
Alcohol Treatment ◽  
Fruit Softening ◽  
Japanese Persimmon

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

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