Monoclonal Antibodies Against Human Osteocalcin Made by Using Recombinant GST-rhOC as an Immunogen

1995 ◽  
pp. 475-479
Author(s):  
M.-T. Matikainen ◽  
S.-M. Käkönen ◽  
J. Hellman ◽  
M. Karp
Keyword(s):  
Monoclonal Antibodies ◽  
Human Osteocalcin

scholarly journals Development and Evaluation of Three Immunofluorometric Assays That Measure Different Forms of Osteocalcin in Serum

2000 ◽  
Vol 46 (3) ◽  
pp. 332-337 ◽  
Author(s):  
Sanna-Maria Käkönen ◽  
Jukka Hellman ◽  
Matti Karp ◽  
Pirjo Laaksonen ◽  
Karl J Obrant ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hormone Replacement Therapy ◽  
Bone Formation ◽  
Diurnal Variation ◽  
Replacement Therapy ◽  
Hormone Replacement ◽  
Control Group ◽  
Plasma Samples ◽  
Edta Plasma ◽  
Human Osteocalcin

Abstract Background: Circulating human osteocalcin (hOC) has been used as a marker of bone formation. Our aim was to validate three immunofluorometric assays (IFMAs), measuring different forms of hOC. Methods: The two-site IFMAs were based on previously characterized monoclonal antibodies. Assay 2 recognized intact hOC, assays 4 and 9 measured the NH2-terminal mid-fragment and the intact hOC. In addition, assay 9 required hOC to be γ-carboxylated. Results: A 76–79% increase of serum immunoreactive hOC was found in the postmenopausal group compared with the premenopausal group with all IFMAs. With EDTA-plasma samples, the observed increases were lower (49–65%). The hOC concentration in the postmenopausal group receiving hormone replacement therapy was 42–44% lower than that in the postmenopausal control group in both serum and EDTA-plasma samples. The depressed carboxylation in warfarin-treated patients was accompanied by lower results in assay 9. The ratio of assay 9 to assay 4 totally discriminated the warfarin-treated patients from the controls. Assay 9 showed the smallest decreases in measured hOC after storage of serum or plasma for 4 weeks at 4 °C, followed by assay 4 and assay 2. Results from the last assay were <17% of their initial values after 4 weeks of storage. No diurnal variation was observed with assay 9 as opposed to the two other IFMAs. Conclusion: The three assays with their distinct specificity profiles (intact vs fragmented and carboxylated vs decarboxylated hOC) may provide valuable tools for investigating the significance of different hOC forms in various bone-related diseases.

Monoclonal antibodies against human osteocalcin made by using recombinant GST-rhOC as immunogen

Bone ◽  
1996 ◽  
Vol 18 (1) ◽  
pp. S105
Author(s):  
S-M Käkönen ◽  
M-T Matikainen ◽  
J Hellman ◽  
M Karp
Keyword(s):  
Monoclonal Antibodies ◽  
Human Osteocalcin

Measurement of a more stable region of osteocalcin in serum by ELISA with two monoclonal antibodies

1995 ◽  
Vol 41 (10) ◽  
pp. 1439-1445 ◽  
Author(s):  
C Rosenquist ◽  
P Qvist ◽  
N Bjarnason ◽  
C Christiansen
Keyword(s):  
Monoclonal Antibodies ◽  
Hormone Replacement ◽  
Premenopausal Women ◽  
Stable Region ◽  
Serum Samples ◽  
Early Postmenopausal Women ◽  
Intact Molecule ◽  
Capture Antibody ◽  
The Mean ◽  
Human Osteocalcin

Abstract Intact human osteocalcin purified from femoral bones as well as tryptic fragments of the intact molecule [amino acids (aa) 1-19, 20-43, and 45-49] were used to raise and screen monoclonal antibodies (MAbs). A two-site ELISA for measurement of human osteocalcin in serum was developed with use of these MAbs. A MAb recognizing midregion human osteocalcin (aa20-43) was used as capture antibody, and an NH2 terminus (aa7-19)-specific peroxidase-conjugated MAb was used for detection. Human osteocalcin obtained from bone was used for calibration, and parallelism was observed for osteocalcin from serum samples, NH2-terminal midfragments (aa1-43), and synthetic human osteocalcin. Both inter- and intraassay variations were < 7%. Serum osteocalcin in healthy premenopausal women (n = 49) was 18.3 +/- 4.2 micrograms/L (mean +/- SD) and 28.6 +/- 9.7 micrograms/L in early postmenopausal women (n = 114). The mean serum concentration (n = 10) decreased by 10% after 7 days of storage at 4 degrees C, whereas the concentration of intact human osteocalcin was reduced 63%. The N-MID ELISA and an IRMA measuring intact human osteocalcin were used to monitor the effect of hormone replacement therapy in a retrospective study. A significant decrease to the premenopausal concentration was detected only in the N-MID ELISA.

Ultrastructural analysis of unique glycoconjugates in the rat olfactory system

1992 ◽  
Vol 50 (1) ◽  
pp. 890-891
Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Olfactory System ◽  
Olfactory Bulbs ◽  
Temporal And Spatial Patterns ◽  
Carbohydrate Epitopes ◽  
Olfactory Systems ◽  
Temporal And Spatial ◽  
Cell Surface Glycoconjugates

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.

Structural and Chemical Studies of Pathological Fibers in Human Neurofibrillary Degeneration

1985 ◽  
Vol 43 ◽  
pp. 726-729
Author(s):  
K.S. Kosik ◽  
L.K. Duffy ◽  
S. Bakalis ◽  
C. Abraham ◽  
D.J. Selkoe
Keyword(s):  
Monoclonal Antibodies ◽  
Alzheimer Disease ◽  
Human Brain ◽  
Neurofibrillary Tangles ◽  
Morphological Analysis ◽  
High Specificity ◽  
Cytoskeletal Proteins ◽  
Neuritic Plaque ◽  
Protein Chemistry ◽  
Chemical Studies

The major structural lesions of the human brain during aging and in Alzheimer disease (AD) are the neurofibrillary tangles (NFT) and the senile (neuritic) plaque. Although these fibrous alterations have been recognized by light microscopists for almost a century, detailed biochemical and morphological analysis of the lesions has been undertaken only recently. Because the intraneuronal deposits in the NFT and the plaque neurites and the extraneuronal amyloid cores of the plaques have a filamentous ultrastructure, the neuronal cytoskeleton has played a prominent role in most pathogenetic hypotheses.The approach of our laboratory toward elucidating the origin of plaques and tangles in AD has been two-fold: the use of analytical protein chemistry to purify and then characterize the pathological fibers comprising the tangles and plaques, and the use of certain monoclonal antibodies to neuronal cytoskeletal proteins that, despite high specificity, cross-react with NFT and thus implicate epitopes of these proteins as constituents of the tangles.

Use of immunoblotting and monoclonal antibodies to evaluate the residual antigenic activity of milk protein hydrolysed formulae

1996 ◽  
Vol 26 (10) ◽  
pp. 1182-1187 ◽  
Author(s):  
P. RESTANI ◽  
A. PLEBANI ◽  
T. VELONA ◽  
G. CAVAGNI ◽  
A. G. UGAZIO ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Milk Protein ◽  
Antigenic Activity

Monoclonal Antibodies for Ca, Asthma, and RA

Ob Gyn News ◽  
2008 ◽  
Vol 43 (4) ◽  
pp. 12 ◽  
Author(s):  
GERALD G. BRIGGS
Keyword(s):  
Monoclonal Antibodies
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