A Reliable Strategy for The Achievement of Cell Lines Growing in Protein-Free Medium

1995 ◽  
pp. 619-623 ◽  
Author(s):  
Klaus Scharfenberg ◽  
Roland Wagner
Keyword(s):  
Cell Lines ◽  
Free Medium ◽  
Protein Free Medium

Adaptation and Selection of NSO Myeloma Cell Lines Producing Recombinant Monoclonal Antibodies in Protein-Free Medium

2001 ◽  
pp. 160-163 ◽  
Author(s):  
Adolfo J. Castillo ◽  
S. Víctores ◽  
L. Rojas ◽  
E. Faife ◽  
Y. Rabasa ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Free Medium ◽  
Protein Free Medium ◽  
Selection Of

scholarly journals Induction of apoptosis by c-Fos protein.

1996 ◽  
Vol 16 (1) ◽  
pp. 211-218 ◽  
Author(s):  
G A Preston ◽  
T T Lyon ◽  
Y Yin ◽  
J E Lang ◽  
G Solomon ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Transcriptional Activation ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Fos Protein ◽  
Induced Apoptosis ◽  
I Cells

The role of c-Fos in apoptosis was examined in two Syrian hamster embryo cell lines (sup+I and sup-II) and a human colorectal carcinoma cell line (RKO), using the chimeric Fos-estrogen receptor fusion protein c-FosER. As previously reported, contrasting responses were observed when these two cell lines were placed under growth factor deprivation conditions; sup+I cells were highly susceptible to apoptosis, whereas sup-II cells were resistant. In this report, we show that the activated c-FosER protein induces apoptosis in sup-II preneoplastic cells in serum-free medium, indicating that c-Fos protein can induce apoptotic cell death in these cells. c-Fos-induced apoptosis was not blocked by the protein synthesis inhibitor cycloheximide, suggesting that the c-Fos transcriptional activation activity is not involved. This conclusion was further supported by the observation that overexpression of v-Fos, which is highly proficient in transcriptional activation but deficient in the transcriptional repression activity associated with c-Fos, did not induce apoptosis. Constitutively expressed Bcl-2 delayed the onset of low-serum-induced apoptosis in sup+I cells and enhanced survival in sup-II cells. Further, coexpression of Bcl-2 and c-FosER in sup+I or sup-II cells protected the cells from c-FosER-induced apoptosis. The possibility that c-FosER-induced apoptosis requires a p53 function was examined. Colorectal carcinoma RKOp53+/+ cells, which do not normally undergo apoptosis in serum-free medium, showed apoptotic DNA fragmentation upon expression and activation of c-FosER. Further, when the wild-type p53 protein was diminished in the RKO cells by infection with the papillomavirus E6 gene, subsequent c-FosER-induced apoptosis was blocked. The data suggest that c-Fos protein plays a causal role in the activation of apoptosis in a p53-dependent manner. This activity does not require new protein synthesis and is blocked by overexpression of Bcl-2 protein.

High Density Culture of a Hybrid-Hybridoma HH1-4-3 in a Protein-Free Medium

1997 ◽  
pp. 185-189
Author(s):  
Hiroshi Shinmoto ◽  
Masuko Kobori ◽  
Tojiro Tsushida ◽  
Kazuki Shinohara
Keyword(s):  
High Density ◽  
Free Medium ◽  
High Density Culture ◽  
Protein Free Medium
Sign in / Sign up

Export Citation Format

Share Document