Highly substituted BHAR:Synthesis and application for large scale SPPS and for anion exchange chromatography

Peptides ◽  
1994 ◽  
pp. 252-253 ◽  
Author(s):  
A. Etchegaray ◽  
R.S.H. Carvalho ◽  
R. Marchetto ◽  
C.R. Nakaie
Keyword(s):  
Anion Exchange ◽  
Large Scale ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography

scholarly journals Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography

PROTEOMICS ◽  
2008 ◽  
Vol 8 (7) ◽  
pp. 1346-1361 ◽  
Author(s):  
Guanghui Han ◽  
Mingliang Ye ◽  
Houjiang Zhou ◽  
Xinning Jiang ◽  
Shun Feng ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Liver Tissue ◽  
Human Liver ◽  
Large Scale ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Human Liver Tissue ◽  
Strong Anion Exchange

Separation of bovine κ-casein glycomacropeptide from sweet whey protein products with undetectable level of phenylalanine by protein precipitation followed by anion exchange chromatography

2018 ◽  
Vol 85 (1) ◽  
pp. 110-113 ◽  
Author(s):  
Takuo Nakano ◽  
Lech Ozimek ◽  
Mirko Betti
Keyword(s):  
Heat Treatment ◽  
Amino Acid ◽  
Whey Protein ◽  
Anion Exchange ◽  
Large Scale ◽  
Dry Weight ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Ph Shift ◽  
Sweet Whey

Bovine κ-casein glycomacropeptide (GMP) found in sweet whey is a 64 amino acid residue glycopeptide, which does not contain phenylalanine or other aromatic amino acids. There is, however, little information available concerning isolation of phenylalanine free GMP from sweet whey. In the study reported in this Research Communication, GMP was purified from three samples of sweet whey protein products (SWPP) by a procedure involving: (1) precipitation of protein by heat treatment; (2) precipitation of protein by pH shift to 4·6; and (3) diethylaminoethyl (DEAE)-Sephacel anion exchange chromatography of soluble portion of each sample obtained after removal of protein precipitates. The total protein precipitated with both heat treatment and pH shift accounted for average 61% of dry weight of SWPP. The GMP fraction obtained by DEAE-Sephacel chromatography accounted for average 7·5% of dry weight of SWPP. Amino acid analysis showed that there was no detectable level of phenylalanine in GMP fractions from all samples examined. The present method may help develop large scale methods of production of GMP.

scholarly journals AAV Purification by anion-exchange chromatography

Bionatura ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Frank Camacho ◽  
R.P Cerro ◽  
N Varas ◽  
M.J Leiva ◽  
Jorge Roberto Toledo ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Large Scale ◽  
High Titer ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Raav Vector ◽  
Empty Capsid ◽  
Ratio Determination ◽  
Reproducible Method ◽  
Elution Step

We describe a new optimized, scalable and reproducible method based on anion exchange chromatography to obtain high titers of rAAV vectors without empty capsids contamination. The method takes advantage of Q-sepharose matriz to development a scalable procedure. After the virus harvest from supernatant and lysate cells, virus crude was subjected to anion exchange chromatography with Q-sepharose column. Three different protocols were tested, and the elution peaks were evaluated through qPCR rAAV titration and 260/280 nm ratio determination in order to identified empty capsid-containing fractions. A 150 mM NH4Ac wash step fallowing by 1 M NaCl elution step generate a a high titer eluted fraction of rAAV with 1.334 260/280 nm ratio. The described method makes rAAV vector purification an easily adapted for a large scale GMP production format to produce empty capsid free rAAV for clinics.

Correlation between protein desorption behavior and its adsorption enthalpy change in polymer grafted anion exchange chromatography

2021 ◽  
pp. 111853
Author(s):  
Joao Carlos Simoes-Cardoso ◽  
Nanako Hoshino ◽  
Yusuke Yoshimura ◽  
Chyi-Shin Chen ◽  
Cristina Dias-Cabral ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Enthalpy Change ◽  
Adsorption Enthalpy

scholarly journals ANION EXCHANGE CHROMATOGRAPHY OF ACIDS OF THE CITRIC ACID CYCLE

1952 ◽  
Vol 196 (2) ◽  
pp. 717-727 ◽  
Author(s):  
Harris Busch ◽  
Robert B. Hurlbert ◽  
Van R. Potter
Keyword(s):  
Citric Acid ◽  
Anion Exchange ◽  
Citric Acid Cycle ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Acid Cycle
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