Functionality of MC88- and MPC85-Enriched Skim Milk: Impact of Shear Conditions in Rotor/Stator Systems and High-Pressure Homogenizers on Powder Solubility and Rennet Gelation Behavior

Milk protein concentrate (MPC) and micellar casein (MC) powders are commonly used to increase the protein concentration of cheese milk. However, highly-concentrated milk protein powders are challenging in terms of solubility. The research question was whether and how incompletely dissolved agglomerates affect the protein functionality in terms of rennet gelation behavior. For the experiments, skim milk was enriched with either MC88 or MPC85 to a casein concentration of 4.5% (w/w) and sheared on a laboratory and pilot scale in rotor/stator systems (colloid mill and shear pump, respectively) and high-pressure homogenizers. The assessment criteria were on the one hand particle sizes as a function of shear rate, and on the other hand, the rennet gelation properties meaning gelling time, gel strength, structure loss upon deformation, and serum loss. Furthermore, the casein, whey protein, and casein macropeptide (CMP) recovery in the sweet whey was determined to evaluate the shear-, and hence, the particle size-dependent protein accessibility. We showed that insufficient powder rehydration prolongs the rennet gelation time, leading to softer, weaker gels, and to lower amounts of CMP and whey protein in the sweet whey.

Efficiency of sweet whey fermentation with psychrophilic methanogens

Sweet whey is a waste product from the dairy industry that is difficult to manage. High hopes are fostered regarding its neutralization in the methane fermentation. An economically viable alternative to a typical mesophilic fermentation seems to be the process involving psychrophilic bacteria isolated from the natural environment. This study aimed to determine the feasibility of exploiting psychrophilic microorganisms in methane fermentation of sweet whey. The experiments were carried out under dynamic conditions using Bio Flo 310 type flow-through anaerobic bioreactors. The temperature inside the reactors was 10 ± 1 °C. The HRT was 20 days and the OLR was 0.2 g COD/dm3/day. The study yielded 132.7 ± 13.8 mL biogas/gCODremoved. The CH4 concentration in the biogas was 32.7 ± 1.6%, that of H2 was 8.7 ± 4.7%, whereas that of CO2 reached 58.42 ± 2.47%. Other gases were also determined, though in lower concentrations. The COD and BOD5 removal efficiency reached 21.4 ± 0.6% and 17.6 ± 1.0%, respectively.

The Effect of the Dietary Inclusion of Crude Glycerin in Pre-Starter and Starter Diets for Piglets

The aim of this study was to evaluate the effect of the inclusion of crude glycerin in post-weaning diets for piglets on growth performance and digestibility. The study was carried out with a total of 360 piglets over a 39 day period. Animals were blocked by body weight (7.7 ± 0.86 kg) and allotted randomly to one of three dietary treatments containing 0, 2.5 or 5% glycerin (G0, G2.5 and G5, respectively). Considering the whole period, glycerin did not affect the average daily gain. However, the average daily feed intake (ADFI) and the feed conversion ratio (FCR) tended to decrease or decreased linearly as the amount of glycerin increased, respectively (p = 0.060 and p = 0.039). The apparent total tract digestibility (ATTD) of dry and organic matter (DM, OM) increased linearly with increasing glycerin in both periods (p ≤ 0.05). At the end of the study, there were no differences between treatments for any of the hormones measured. In conclusion, the FCR and digestibility of DM and OM were improved although the ADFI tended to be lower when glycerin was included at 5%. Consequently, crude glycerin could be used as an alternative ingredient to partially replace sweet whey and wheat in post-weaning diets.