Characterization of alfalfa (Medicago sativa L.) following in vitro selection for salt tolerance

1997 ◽  
pp. 69-75
Author(s):  
A. Safarnejad ◽  
H. A. Collin ◽  
K. D. Bruce ◽  
T. McNeilly
Keyword(s):  
Salt Tolerance ◽  
Medicago Sativa ◽  
In Vitro Selection ◽  
Medicago Sativa L ◽  
Selection For

Characterization of alfalfa (Medicago sativa L.) following in vitro selection for salt tolerance

Euphytica ◽  
1996 ◽  
Vol 92 (1-2) ◽  
pp. 55-61 ◽  
Author(s):  
A. Safarnejad ◽  
H. A. Collin ◽  
K. D. Bruce ◽  
T. McNeilly
Keyword(s):  
Salt Tolerance ◽  
Medicago Sativa ◽  
In Vitro Selection ◽  
Medicago Sativa L ◽  
Selection For

Characterization of progeny of Coleus blumei following an in vitro selection for salt tolerance

1992 ◽  
Vol 28 (2) ◽  
pp. 139-145 ◽  
Author(s):  
K. M. Ibrahim ◽  
J. C. Collins ◽  
H. A. Collin
Keyword(s):  
Salt Tolerance ◽  
In Vitro Selection ◽  
Coleus Blumei ◽  
Selection For

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

2013 ◽  
Vol 8 (8) ◽  
pp. 765-776 ◽  
Author(s):  
Angela Campanelli ◽  
Claudia Ruta ◽  
Irene Morone-Fortunato ◽  
Giuseppe Mastro
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Medicago Sativa ◽  
In Vitro Selection ◽  
Growth Parameters ◽  
Sodium Content ◽  
Saline Stress ◽  
Survival Percentage ◽  
Medicago Sativa L

AbstractIn order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

Identification and selection for salt tolerance in lucerne (Medicago sativa L.)

1984 ◽  
Vol 35 (2) ◽  
pp. 239 ◽  
Author(s):  
CL Noble ◽  
GM Halloran ◽  
DW West
Keyword(s):  
Salt Tolerance ◽  
Medicago Sativa ◽  
Recurrent Selection ◽  
Leaf Damage ◽  
Shoot Number ◽  
Two Generations ◽  
Medicago Sativa L ◽  
Selection For ◽  
Efficiency Of Selection ◽  
Chloride Concentrations

Variation existed between plants of the lucerne (Medicago sativa L.) cultivar CUF 101 for dry matter production, shoot number and length, and leaf damage when grown for 70 days under 250 mM NaCl (15 h photoperiod, 20�C day, 10�C night). Salt tolerance evaluation using the criteria percentage leaf damage (percentage of total number of leaves with complete or partial necrosis) and length of the main shoot, isolated plants which showed salt tolerance of reasonably high heritability (h2=0.41). Two generations of recurrent selection for tolerance significantly increased the mean population tolerance without decreasing production under non-saline conditions. While both sodium and chloride concentrations of the shoot were lower in the tolerant than in less tolerant plants, chloride was more closely associated with salt tolerance than sodium. Sodium and chloride concentrations in the roots did not vary with the level of salt tolerance. No association of shoot and root potassium concentration with tolerance was evident. Selection for salt tolerance in lucerne plants using percentage leaf damage of less than 10% as the main criterion should give a rapid response to selection. The efficiency of selection may be increased if selection is based on the efficiency of chloride exclusion from the shoots and/or the level of chloride tolerated by the shoots prior to leaf damage becoming evident.

In Vitro Selection for Salt Tolerance in Rice

2000 ◽  
Vol 43 (3) ◽  
pp. 477-480 ◽  
Author(s):  
D. Shankhdhar ◽  
S.C. Shankhdhar ◽  
S.C. Mani ◽  
R.C. Pant
Keyword(s):  
Salt Tolerance ◽  
In Vitro Selection ◽  
Selection For

In-Vitro Selection for Salt Tolerance of Taro (Colocasia esculenta var antiquorum)

1983 ◽  
Vol 51 (2) ◽  
pp. 229-236 ◽  
Author(s):  
L. P. NYMAN ◽  
C. J. GONZALES ◽  
J. ARDITTI
Keyword(s):  
Salt Tolerance ◽  
In Vitro Selection ◽  
Colocasia Esculenta ◽  
Selection For
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