In Vitro Regeneration of Salt-Tolerant Plants

2016 ◽  
pp. 299-307
Author(s):  
Remya Mohanraj
Keyword(s):  
In Vitro Regeneration ◽  
Salt Tolerant ◽  
Tolerant Plants

scholarly journals Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Oliveira ◽  
Caroline Sprengel Lima ◽  
Setha Ketavong ◽  
Eulogio J. Llorent-Martínez ◽  
Hervé Hoste ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Bioactive Metabolites ◽  
Ionization Mass ◽  
Inhibition Assay ◽  
Salt Tolerant ◽  
Egg Hatching ◽  
Tolerant Plants ◽  
Major Interest

AbstractStrategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include the search for novel anthelmintic scaffolds on plants, yet salt-tolerant plants remain overlooked. This study aims to evaluate the in vitro anthelmintic properties of selected salt-tolerant plants against GIN, and identify the potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass of aerial organs of nine salt-tolerant plant species and tested against Haemonchus contortus and Trichostrongylus colubriformis by the Larval Exsheathment Inhibition Assay (LEIA) and Egg Hatching Inhibition Assay (EHIA). Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscus and Helychrisum italicum picardi were the most active in both GIN and life stages. To investigate the role of polyphenols in the anthelmintic activity, four selected extracts were treated with polyvinylpolypyrrolidone (PVPP), and non-treated and treated samples were further characterized by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). While polyphenols seem responsible for the EHIA properties, they are partially accountable to LEIA results. Several phenolics involved in the anthelmintic effects were identified and discussed. In sum, these species are rich sources of anthelmintic compounds and, therefore, are of major interest for nutraceutical and/or phytotherapeutic applications against GIN in ruminants.

Disclosing the Bioactive Metabolites Involved in the in Vitro Anthelmintic Effects of Mediterranean Salt-Tolerant Plants: A Combined Approach Using PVPP and HPLC-ESI-MSn

2021 ◽  
Author(s):  
Marta Oliveira ◽  
Caroline Sprengel Lima ◽  
Ketavong Setha ◽  
Eulogio J. Llorent-Martínez ◽  
Hervé Hoste ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Bioactive Metabolites ◽  
Ionization Mass ◽  
Inhibition Assay ◽  
Salt Tolerant ◽  
Egg Hatching ◽  
Tolerant Plants ◽  
Major Interest

Abstract Strategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include the search for novel anthelmintic scaffolds on plants, yet salt tolerant plants remain overlooked. The aims of this study are to evaluate the in vitro anthelmintic properties of selected salt-tolerant plants against GIN, and identify the potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass of aerial organs of nine salt tolerant plant species and tested against Haemonchus contortus and Trichostrongylus colubriformis by the Larval Exsheathment Inhibition Assay (LEIA) and Egg Hatching Inhibition Assay (EHIA). Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscus and Helychrisum italicum picardi were the most active in both GIN and life stages. To investigate the role of polyphenols in the anthelmintic activity, four selected extracts were treated with PVPP, and non-treated and treated samples were further characterized by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). While polyphenols seem responsible for the EHIA properties, they are partially accountable to LEIA results. Several phenolics involved in the anthelmintic effects were identified and discussed. In sum, these species are rich sources of anthelmintic compounds and, therefore, are of major interest for nutraceutical and/or phytotherapeutic applications against GIN in ruminants.

scholarly journals In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation

2018 ◽  
Vol 28 (1) ◽  
pp. 125-140
Author(s):  
Nuram Mubina ◽  
MI Hoque ◽  
RH Sarker
Keyword(s):  
Genetic Transformation ◽  
Cicer Arietinum ◽  
Zygotic Embryo ◽  
In Vitro Regeneration ◽  
Dna Helicase ◽  
Salt Tolerant ◽  
Agrobacterium Strain ◽  
Cicer Arietinum L ◽  
Gus Histochemical Assay

In vitro regeneration studies compatible to Agrobacterium-mediated genetic transformation were carried out using two different types of zygotic embryo derived explants namely, decapitated embryo (DE) and decapitated embryo with single cotyledon disc (DEC) from three varieties of chickpea (Cicer arietinum L.) such as BARI chhola-4, -5 and -9 cultivated in Bangladesh. The best responses towards in vitro shoot regeneration was obtained from decapitated embryo with DEC on MS containing 0.5 mg/l BAP, 0.5 mg/l Kn and 0.2 mg/l NAA. Healthy and effective roots from the regenerated shoots were developed on MS supplemented with 0.2 mg/l IBA. Genetic transformation was carried out with Agrobacterium strain LBA4404 containing the binary plasmid pCAMBIA1301- PDH45 to integrate salt tolerant PDH45 gene in locally grown varieties of chickpea. The transformed plantlets were successfully established in soil following adequate hardening. Integration of salt tolerant PDH45 gene within the genomic DNA was confirmed through GUS histochemical assay and PCR analysis.Plant Tissue Cult. & Biotech. 28(1): 125-140, 2018 (June)

Direct in vitro Regeneration of Arachis Hypogaea L.(Jl-24) and Study of Salt Tolerance

2011 ◽  
Vol 3 (8) ◽  
pp. 52-54 ◽  
Author(s):  
Bhagya Lakshmi Jyothi Kusuma ◽  
◽  
Sharmila Begum S
Keyword(s):  
Salt Tolerance ◽  
Arachis Hypogaea ◽  
In Vitro Regeneration ◽  
Arachis Hypogaea L

In vitro regeneration of watermelon seed segments

2015 ◽  
Vol 4 (8) ◽  
Author(s):  
M. Ameri ◽  
M. Lahouti ◽  
A. Bagheri ◽  
A. Sharifi ◽  
F. Keykha Akhar
Keyword(s):  
In Vitro Regeneration ◽  
Watermelon Seed
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