scholarly journals Proteomic Analysis of Venomous Fang Matrix Proteins of Protobothrops flavoviridis (Habu) Snake

2018 ◽  
pp. 39-54
Author(s):  
Tomohisa Ogawa ◽  
Asa Sekikawa ◽  
Hajime Sato ◽  
Koji Muramoto ◽  
Hiroki Shibata ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Matrix Proteins

scholarly journals Proteomic analysis reveals some common proteins in the kidney stone matrix

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11872
Author(s):  
Yuanyuan Yang ◽  
Senyuan Hong ◽  
Cong Li ◽  
Jiaqiao Zhang ◽  
Henglong Hu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Kidney Stone ◽  
Renal Tissue ◽  
Matrix Proteins ◽  
Complement C3 ◽  
Urinary Stones ◽  
Protein Z ◽  
Kegg Analysis ◽  
Significant Difference ◽  
Stone Matrix

Background Proteins are the most abundant component of kidney stone matrices and their presence may reflect the process of the stone’s formation. Many studies have explored the proteomics of urinary stones and crystals. We sought to comprehensively identify the proteins found in kidney stones and to identify new, reliable biomolecules for use in nephrolithiasis research. Methods We conducted bioinformatics research in November 2020 on the proteomics of urinary stones and crystals. We used the ClusterProfiler R package to transform proteins into their corresponding genes and Ensembl IDs. In each study we located where proteomic results intersected to determine the 20 most frequently identified stone matrix proteins. We used the Human Protein Atlas to obtain the biological information of the 20 proteins and conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) analysis to explore their biological functions. We also performed immunohistochemistry to detect the expression of the top five stone matrix proteins in renal tissue. Results We included 19 relevant studies for analysis. We then identified 1,409 proteins in the stone matrix after the duplicates were removed. The 20 most-commonly identified stone matrix proteins were: S100A8, S100A9, uromodulin, albumin, osteopontin, lactotransferrin, vitamin K-dependent protein Z, prothrombin, hemoglobin subunit beta, myeloperoxidase, mannan-binding lectin serine protease 2, lysozyme C, complement C3, serum amyloid P-component, cathepsin G, vitronectin, apolipoprotein A-1, eosinophil cationic protein, fibrinogen alpha chain, and apolipoprotein D. GO and KEGG analysis revealed that these proteins were typically engaged in inflammation and immune response.Immunohistochemistry of the top five stone matrix proteins in renal tissue showed that the expression of S100A8, S100A9, and osteopontin increased, while uromodulin decreased in kidney stone patients. Albumin was rarely expressed in the kidney with no significant difference between healthy controls and kidney stone patients. Conclusion Proteomic analysis revealed some common inflammation-related proteins in the kidney stone matrix. The role of these proteins in stone formation should be explored for their potential use as diagnostic biomarkers and therapeutic targets for urolithiasis.

Comparative proteomic analysis of extracellular matrix proteins secreted by two types of skin fibroblasts

PROTEOMICS ◽  
2006 ◽  
Vol 6 (21) ◽  
pp. 5868-5879 ◽  
Author(s):  
Delphine Pflieger ◽  
Sandrine Chabane ◽  
Olivier Gaillard ◽  
Bruno Alain Bernard ◽  
Patrick Ducoroy ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Proteomic Analysis ◽  
Extracellular Matrix Proteins ◽  
Skin Fibroblasts ◽  
Matrix Proteins ◽  
Comparative Proteomic Analysis

scholarly journals In-depth proteomic analysis of shell matrix proteins of Pinctada fucata

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Chuang Liu ◽  
Shiguo Li ◽  
Jingjing Kong ◽  
Yangjia Liu ◽  
Tianpeng Wang ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Pinctada Fucata ◽  
Matrix Proteins ◽  
Shell Matrix ◽  
Shell Matrix Proteins

Proteomic Analysis to Examine the Role of Matrix Proteins in a Gouty Tophus from a Patient with Recurrent Gout

2014 ◽  
Vol 33 (4-6) ◽  
pp. 199-207 ◽  
Author(s):  
Kiyoko Kaneko ◽  
Hiro Iwamoto ◽  
Makoto Yasuda ◽  
Katsunori Inazawa ◽  
Noriko Yamaoka ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Matrix Proteins ◽  
Gouty Tophus

Distribution of VLA-5 and VLA-4 fibronectin receptors in human monocytes demonstrated by immunofluorescence, immunocytochemistry, and autoradiography

1993 ◽  
Vol 51 ◽  
pp. 306-307
Author(s):  
Robert Williams ◽  
Che-Hung Lee ◽  
Sara E. Quella ◽  
David M. Harlan ◽  
Yuan-Hsu Kang
Keyword(s):  
Present Report ◽  
Matrix Proteins ◽  
Extracellular Matrices ◽  
Human Monocytes ◽  
Integrin Receptors ◽  
Morphological Evaluation ◽  
The Matrix ◽  
Specific Receptors ◽  
Cytokine Stimulation ◽  
Monocyte Adherence

Monocyte adherence to endothelial or extracellular matrices plays an important role in triggering monocyte activation in extravascular sites of infection, chronic inflammatory disorders, and tissue damage. Migration of monocytes in the tissues involves the response to a chemoattractant and movement by a series of attachments and detachments to the extracellular matrices which are regulated by expression and distribution of specific receptors for the matrix proteins such as fibronectin (FN). The VSAs (very late antigens or beta integrins), a subfamily of the transmembrane heterodimeric integrin receptors, have been thought to play a major role in monocyte adherence to the extracellular matrices and cells. In this subfamily, VLA-5 and VLA-4 are believed to be the most essential integrins mediating monocyte adherence to FN. In the present report, we have established and compared different procedures for morphological evaluation of the expression and distribution of the FN receptors on human monocytes in order to investigate their response to endotoxin or cytokine stimulation.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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