Atomic Force Microscopy: A Nanoscopic Application in Molecular and Cell Biology

2018 ◽  
pp. 77-103 ◽  
Author(s):  
Huai-Hong Cai ◽  
Xueyi Zeng ◽  
Xiao Tang ◽  
Jiye Cai
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Biology ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcos Penedo ◽  
Tetsuya Shirokawa ◽  
Mohammad Shahidul Alam ◽  
Keisuke Miyazawa ◽  
Takehiko Ichikawa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Cell Biology ◽  
Cell Damage ◽  
Efficiency Analysis ◽  
Living Cells ◽  
Biomolecular Recognition ◽  
Cell Penetration ◽  
Force Microscopy ◽  
Atomic Force

AbstractOver the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.

scholarly journals Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology

2008 ◽  
Vol 456 (1) ◽  
pp. 139-153 ◽  
Author(s):  
Yasuhiro Hirano ◽  
Hirohide Takahashi ◽  
Masahiro Kumeta ◽  
Kohji Hizume ◽  
Yuya Hirai ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Biology ◽  
Nuclear Architecture ◽  
Chromatin Dynamics ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Gloss phenomena and image analysis of atomic force microscopy in molecular and cell biology

Scanning ◽  
2009 ◽  
Vol 31 (2) ◽  
pp. 49-58 ◽  
Author(s):  
Jie Zhu ◽  
Tanya Sabharwal ◽  
Lianhong Guo ◽  
Aruna Kalyanasundaram ◽  
Guodong Wang
Keyword(s):  
Atomic Force Microscopy ◽  
Image Analysis ◽  
Cell Biology ◽  
Force Microscopy ◽  
Atomic Force

Development of Insulated Conductive Probes with Platinum Silicide Tips for Atomic Force Microscopy in Cell Biology

2004 ◽  
Vol 43 (6B) ◽  
pp. 3865-3867 ◽  
Author(s):  
Terunobu Akiyama ◽  
Maurizio R. Gullo ◽  
Nicolaas F. de Rooij ◽  
Andreas Tonin ◽  
Hans-Rudolf Hidber ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Biology ◽  
Force Microscopy ◽  
Platinum Silicide ◽  
Atomic Force

scholarly journals Imaging modes of atomic force microscopy for application in molecular and cell biology

2017 ◽  
Vol 12 (4) ◽  
pp. 295-307 ◽  
Author(s):  
Yves F. Dufrêne ◽  
Toshio Ando ◽  
Ricardo Garcia ◽  
David Alsteens ◽  
David Martinez-Martin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Biology ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Identifying Dynamic Membrane Structures with Atomic-Force Microscopy and Confocal Imaging

2014 ◽  
Vol 20 (2) ◽  
pp. 514-520 ◽  
Author(s):  
Tobias Timmel ◽  
Markus Schuelke ◽  
Simone Spuler
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Biology ◽  
Human Fibroblasts ◽  
Correction Method ◽  
Confocal Imaging ◽  
Membrane Structures ◽  
Coated Pits ◽  
Force Microscopy ◽  
Combined Application ◽  
Atomic Force

AbstractCombining the biological specificity of fluorescence microscopy with topographical features revealed by atomic force microscopy (AFM) provides new insights into cell biology. However, the lack of systematic alignment capabilities especially in scanning-tip AFM has limited the combined application approach as AFM drift leads to increasing image mismatch over time. We present an alignment correction method using the cantilever tip as a reference landmark. Since the precise tip position is known in both the fluorescence and AFM images, exact re-alignment becomes possible. We used beads to demonstrate the validity of the method in a complex artificial sample. We then extended this method to biological samples to depict membrane structures in fixed and living human fibroblasts. We were able to map nanoscale membrane structures, such as clathrin-coated pits, to their respective fluorescent spots. Reliable alignment between fluorescence signals and topographic structures opens possibilities to assess key biological processes at the cell surface such as endocytosis and exocytosis.

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