The microRNAs Expression Profile in Sciatic Nerves of Diabetic Neuropathy Rats After Taurine Treatment by Sequencing

2019 ◽  
pp. 935-947
Author(s):  
Xiaoxia Shi ◽  
Zewen Qiu ◽  
Inam-u-llah ◽  
Mengren Zhang ◽  
Kaixin Li ◽  
...  
Keyword(s):  
Diabetic Neuropathy ◽  
Expression Profile ◽  
Taurine Treatment ◽  
Sciatic Nerves

Abstract 241: Direct Injection of Bone Marrow Mononuclear Cells Reversed Experimental Diabetic Neuropathy by Augmenting Neovascularization and Providing Angio-neurotrophic Cytokines

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Hyongbum Kim ◽  
Yong Jin Choi ◽  
Jong-seon Park ◽  
Masaaki Ii ◽  
Marcy Silver ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Diabetic Neuropathy ◽  
Neurotrophic Factors ◽  
Mononuclear Cells ◽  
Motor Nerve ◽  
Therapeutic Option ◽  
Direct Injection ◽  
Saline Group ◽  
Vasa Nervorum ◽  
Sciatic Nerves

Background: Bone marrow (BM)-derived mononuclear cells (MNCs) have been shown to effectively treat ischemic cardiovascular diseases. Recent evidence suggested that diabetic neuropathy (DN) is causally related to impaired angio-vasculogenesis in vasa nervorum and deficiency of angiogenic and neurotrophic factors. Accordingly, we sought to investigate whether DN can be ameliorated by local injection of BM-derived MNCs. Methods and Results: A severe peripheral neuropathy, characterized by significant slowing of motor nerve conduction velocities (MCV) developed 12 wks after the induction of diabetes with streptozotocin in Fisher rats (vs normal rats; 46.6±2.6 vs 32.0±2.5 m/s, P < 0.05). These rats were randomly assigned to MNC or saline injection groups (n = 9, each group) and received either 5x106 MNCs or saline intramuscularly around the sciatic nerves. In the MNC group, MCV were significantly improved within 4 wks after treatment (MNC vs Saline, 41.9±3.2 vs 32.7±2.8 m/s, P < 0.01). Microvascular circulation of sciatic nerve, measured by laser Doppler perfusion imaging was markedly increased only in the MNC group. Capillary density at 4 wks was significantly higher in the MNC group than in the saline group (68±5.9 vs 37±4.4 /cross section, P < 0.01). Robust engraftment of MNCs were observed in sciatic nerves, which sustained over 4 wks. A fraction of engrafted MNCs expressed endothelial markers suggestive of transdifferentiation into endothelial cells in the vasa nervorum. Intriguingly, a large number of the engrafted MNCs are following the course of vasa nervorum in close proximity. Real-time RT-PCR on sciatic nerves revealed that the expression of angio-neurotrophic factors were significantly increased in the MNC group compared to the saline group: VEGF (2.1 fold), FGF-2 (2.4), eNOS (18.1), Brain-derived neurotrophic factor (35.1), IGF-1 (25.5) (all P < 0.05). The protein levels were well correlated with mRNA expression levels. Conclusion: Local transplantation of BM-derived MNCs could improve experimental DN by augmenting neovascularization and increasing angiogenic and neurotrophic factors in peripheral nerves. These findings suggest that BM-MNC transplantation may represent a novel therapeutic option for treating DN.

Transplantation of human mobilized mononuclear cells improved diabetic neuropathy

2018 ◽  
Vol 239 (3) ◽  
pp. 277-287
Author(s):  
Se Hee Min ◽  
Jung Hee Kim ◽  
Yu Mi Kang ◽  
Seung Hak Lee ◽  
Byung-Mo Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Diabetic Neuropathy ◽  
Mononuclear Cells ◽  
Related Factor ◽  
Paracrine Effects ◽  
Muscle Perfusion ◽  
Hind Limbs ◽  
Compound Muscle Action Potentials ◽  
Sciatic Nerves

Rodent stem cells demonstrated regenerative effects in diabetic neuropathy via improvement in nerve perfusion. As a pre-clinical step, we explored if human mobilized mononuclear cells (hMNC) would have the same effects in rats. hMNC were injected into Rt. hind-limb muscles of streptozotocin-induced diabetic nude rats, and the grafts were monitored using with MRI. After 4 weeks, the effects were compared with those in the vehicle-injected Lt. hind limbs. Nerve conduction, muscle perfusion and gene expression of sciatic nerves were assessed. Induction of diabetes decreased nerve function and expression of Mpz and Met in the sciatic nerves, which are related with myelination. hMNC injection significantly improved the amplitude of compound muscle action potentials along with muscle perfusion and sciatic nerve Mpz expression. On MRI, hypointense signals were observed for 4 weeks at the graft site, but their correlation with the presence of hMNC was detectable for only 1 week. To evaluate paracrine effects of hMNC, IMS32 cells were tested with hepatocyte growth factor (HGF), which had been reported as a myelination-related factor from stem cells. We could observe that HGF enhanced Mpz expression in the IMS32 cells. Because hMNC secreted HGF, IMS32 cells were co-cultured with hMNC, and the expression of Mpz increased along with morphologic maturation. The hMNC-induced Mpz expression was abrogated by treatment of anti-HGF. These results suggest that hMNC could improve diabetic neuropathy, possibly through enhancement of myelination as well as perfusion. According to in vitro studies, HGF was involved in the hMNC-induced myelination activity, at least in part.

The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy

2017 ◽  
Vol 21 (1) ◽  
pp. 10-19 ◽  
Author(s):  
Ali Khazani ◽  
Reza Gharakhanlou ◽  
Mohamad Reza Kordi ◽  
Mansoureh Movahedin ◽  
Shiva Jahani Golbar ◽  
...  
Keyword(s):  
Diabetic Neuropathy ◽  
Protein Expression ◽  
Endurance Training ◽  
Motor Protein ◽  
Male Rats ◽  
Dynein Motor ◽  
Sciatic Nerves ◽  
Wistar Male Rats

An Unbiased Approach to Identifying Changes in the Gene Expression Profile of Sensory Neurons in Painful Diabetic Neuropathy

2021 ◽  
Vol 22 (5) ◽  
pp. 580-581
Author(s):  
Dale George ◽  
Nirupa Jayaraj ◽  
Dongjun Ren ◽  
Richard Miller ◽  
Daniela Menichella
Keyword(s):  
Gene Expression ◽  
Diabetic Neuropathy ◽  
Gene Expression Profile ◽  
Sensory Neurons ◽  
Expression Profile ◽  
Painful Diabetic Neuropathy

(212) Gene Expression Profile of Nociceptor Hyper-Excitability in Painful Diabetic Neuropathy

2019 ◽  
Vol 20 (4) ◽  
pp. S28
Author(s):  
D. George ◽  
N. Jayaraj ◽  
D. Ren ◽  
R. Miller ◽  
D. Menichella
Keyword(s):  
Gene Expression ◽  
Diabetic Neuropathy ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Painful Diabetic Neuropathy

scholarly journals Effects of Insulin on Fibronectin Alterations in Sciatic Nerve of Diabetic Rats-A Brief Report

2020 ◽  
Author(s):  
Manochehr Safari ◽  
Niloofar Aldaghi ◽  
Hamid Reza Sameni ◽  
Mohammad Reza Aldaghi
Keyword(s):  
Sciatic Nerve ◽  
Basement Membrane ◽  
Diabetic Neuropathy ◽  
Diabetic Rats ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Polymerase Chain ◽  
Sciatic Nerves ◽  
Diabetes Induction

Objective: Alteration in the basement membrane proteins maybe associated with diabetic neuropathy. Fibronectin is one of the most important components of peripheral nerves basement membrane. In this study we investigated the effects of insulin administration on prevention of alteration in fibronectin contents of sciatic nerve in diabetic rats. Materials and Methods: Twenty-four wistar rats were divided into control, diabetic and diabetic with insulin treatment groups. Three months after diabetes induction, we measured blood glucose level, body weight and then expression of fibronectin in sciatic nerves of rats were evaluated by real time polymerase chain reaction (PCR) and immunohistochemical study. Results: Intensity of fibronectin immunoreactivity in the perineurium and endoneurium of sciatic nerves significantly increased in diabetic without treatment group compared to control group (P-value< 0.001). Conclusion: This finding suggested that diabetic neuropathy resulted in increased of fibronectin contents in sciatic nerves of rats.  

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