Effect of Black Grape Seed(Vitis Vinifera) Extract Consumption with Moderate-Intensity Aerobic Training on the Expression of MicroRNAs in Type 1 Diabetic Cardiac Tissue Rats

Objective: This study aimed to evaluate the effect of consuming grape seed extract with moderate-intensity aerobic training on the expression of miR-126 and miR-29 in the cardiac tissue in type 1 diabetic male rats. Materials and Methods: 40 rats with an initial weight range of 160-220 g were divided into five groups: Training + Extract, Training, Extract, Diabetic / Control, and Healthy / Control. Aerobic training program was moderate intensity and rats performed aerobic training for 60 minutes a day with the intensity 70 to 75% of maximum oxygen consumption (28 meters per minute). Grape seed extract was also administered by gavage at a dose of 40 mg/kg per day. Results: Expression of both miRNAs in the three groups of training + extract, healthy training and control was significantly higher than the two groups of extract and diabetic control (P-value= 0.001). The difference between the three groups of training + extract, healthy training and control and also the difference between the two groups of extract and diabetic control were not significant (P-value> 0.05). Conclusion: Aerobic training may be able to prevent cardiac disease caused by type 1 diabetes.

Access to Anti-Diabetic Medicines among Patients Attending Tertiary Health Facilities in Oyo State, Nigeria

Objective: The management of Diabetes Mellitus (DM) is long-term and can be expensive. This study aimed to describe access to anti-diabetic medicines among patients in tertiary health care facilities in Oyo State, Nigeria. Materials and Methods: This was a hospital-based cross-sectional study. The study sites were the University College Hospital (UCH), Ibadan, and Ladoke Akintola University Teaching Hospital, Ogbomosho. It was conducted among 366 diabetic patients who had been attending the endocrinology clinic of these two tertiary health institutions for at least one year. Data analysis was done with the Statistical Program for Social Sciences. Results: Female participants were 186 (50.8%) and 298 (85.6%) depended on close relatives to purchase their drugs. Those who had difficulties affording their anti-diabetic medications were 142 (38.8%) and 80 (56.3%) preferred buying a cheaper brand of anti-diabetic medication. Also, 136 (37.2%) respondents had missed their required drugs at some point due to insufficient funds. Males who were able to afford their anti-diabetic medication were 96 (53.3%) compared to 128 (68.8%) females (P-value= 0.002). Those with no formal education had the highest proportion of respondents who could not easily afford anti-diabetic drugs (60.9%), those with tertiary education had the highest proportion of respondents who could afford their medication (77.5%), (P-value=< 0.001). Among those in the 61-70 years age group, 78 (66.1%) achieved glycaemic control compared to 34 (42.5%) of those above 70 years (P-value=< 0.001). Conclusion: To improve affordability, subsidizing the cost of anti-diabetic medications and encouraging patients to enrol in the Health Insurance Scheme will be beneficial.

Non-Invasive Determination of Blood Glucose Levels by Optical Waveguide

Objective: Today, there are various non-invasive techniques available for the determination of blood glucose levels. In this study, the level of blood glucose was determined by developing a new device using near-infrared (NIR) wavelength, glass optical waveguide, and the phenomenon of evanescent waves. Materials and Methods: The body's interstitial fluid has made possible the development of new technology to measure the blood glucose. As a result of contacting the fingertip with the body of the borehole rod, where electromagnetic waves are reflected inside, evanescent waves penetrate from the borehole into the skin and are absorbed by the interstitial fluid. The electromagnetic wave rate absorption at the end of the borehole rod is investigated using a detection photodetector, and its relationship to the people's actual blood glucose level. Following precise optimization and design of the glucose monitoring device, a statistical population of 100 participants with a maximum blood glucose concentration of 200 mg/dL was chosen. Before measurements, participants put their index finger for 30 seconds on the device. Results: According to this experimental study, the values measured by the innovative device with Clark grid analysis were clinically acceptable in scales A and B. The Adjusted Coefficient of Determination of the data was estimated to be 0.9064. Conclusion: For future investigations, researchers are recommended to work with a larger statistical population and use error reduction trends to improve the accuracy and expand the range of measurements.

Reduced Lung Function and Progression to Prediabetes: A Prospective Study

Objective: Prediabetes is a state that people have blood glucose levels higher than normal but still not in diabetes range. There is a close relationship between impaired lung function and diabetes mellitus (DM). Reduced lung function can be present before the clinical evidence of diabetes or insulin resistance. Materials and Methods: The total number of subjects in this longitudinal study was 503 and compared with apparently healthy Kashmiri adults. All the subjects, at the time of their first visit, underwent Fasting Plasma Glucose (FPG) estimation, 2- hour oral glucose tolerance test (OGTT) and spirometry (FVC, FEV1 & FEV1/FVC). Those subjects who had normal glucose tolerance (NGT) were retested for glycemic status and spirometric values after a follow-up period of 2-18 (mean=10) months. Results: Out of total 503 subjects on follow up 483 (96%) had NGT and 20 (4%) had prediabetes. Percent predicted forced vital capacity (FVC) and % predicted forced expiratory volume in 1st second (FEV1) were significantly lower (P-value< 0.001) while as % predicted FEV1/FVC was significantly higher (P-value< 0.001) in prediabetes as compared to NGT group. Conclusion: Results of our study point out a predominantly restrictive pattern of lung dysfunction in the prediabetes group as compared to the NGT group.

Association of Different Genotypes of VEGF Gene with Changes in Aerobic Capacity Following Endurance Training in Obese Women

Objective: Obesity is a chronic disease characterized by an excessive mass of adipose tissue in the body. The present study aimed to investigate the relationship between different genotypes of VEGF gene and changes in aerobic capacity following aerobic exercise in obese women. Materials and Methods: In this study, 23 inactive women aged 34 to 43 years with BMI 30 and 35 were purposefully selected and participated in eight weeks of aerobic exercise including 4 sessions per week and 30 minutes per session with an intensity of 55 to 75% of maximum heart rate. Before and after the training period, aerobic power (VO2max) was measured by the modified Bruce test. Saliva Sample was collected at 12 hours of fasting to measure VEGF genotypes. To compare aerobic capacity between different genotypes, since we had three genotypes GG, CG, and CC, one-way analysis of variance was used. Results: Although the mean amount of aerobic power changes of GG genotype was somewhat higher after eight weeks of aerobic training than the other two genotypes, this difference was not significant. (P-value= 0.663, P-value= 0.873 and P-value= 0.173, respectively). Conclusion: Eight weeks of aerobic training leads to increased aerobic capacity in obese women and increased VEGF plays a role, but there is not seemingly a difference between different VEGF genotypes for these changes. In any case, since this study was conducted for the first time, we need more studies to draw a more accurate conclusion.

First Trimester Potential Biochemical Predictive Tests for Gestational Diabetes Mellitus: A Systematic Review

Objective: Gestational diabetes mellitus (GDM) is the most common metabolic complication during pregnancy. So, a large number of studies have evaluated the usefulness of different screening tests. The aim of this study was focused on the potential of only first-trimester screening used in the prediction of GDM. Materials and Methods: In this systematic review, we searched PubMed, EMBASE, and Scopus (between 2010 and 2020) and also searched the reference lists of the relevant articles manually. After performing a thorough evaluation of the 242 potentially eligible papers, only 60 papers were selected in terms of the inclusion criteria. Search key terms were combining ‘Gestational diabetes’ or ‘GD’ “gestational diabetes mellitus” or” GDM” or pregnancy-induced diabetes’ with at least one of the following terms: “screening test”, “first-trimester”, “prediction”, “marker predictor”, “serum marker”. Results: A total of 161954 pregnant women were evaluated in these reviewed studies. Moreover, many tests were assessed in the first trimester of pregnancy to predict GDM. This review showed that hs-CRP, FPG, TG, and LDL-C along with maternal BMI in the first trimester were related to the increased risk of developing GDM. Other tests were used in only one or two studies. Conclusion: This review showed that hs-CRP, FPG, TG, and LDL-C along with maternal BMI in the first trimester were linked to an increased risk of developing GDM. It is recommended that further well-designed studies by considering the cost-effective advantages of these predictive tests, should be performed.

Investigating the rs2237892 and rs231362 Polymorphisms of KCNQ1 Gene Associations with Type 2 Diabetes in an Iranian Population (Yazd Province)

Objective: Type 2 diabetes (T2DM) is a worldwide prevalent metabolic disorder and the cause of many morbidities and mortalities. KCNQ1 gene encodes α-subunit of voltage-gated potassium (K+) channel which plays a role in insulin secretion in the pancreas, thus its variants may confer susceptibility to diabetes. Recognition of genetic variants involved in T2DM could help the early diagnosis and prevention of the disease. The main purpose of this paper was to investigate the frequencies of rs231362 and rs2237892 polymorphisms of KCNQ1 gene in T2DM patients and comparing these frequencies with normal subjects in an Iranian population from Yazd province, Iran. Materials and Methods: This case-control study was conducted on 166 patients with T2DM and 168 normal subjects. After obtaining the informed consent, 5 ml peripheral blood was taken from the cases and controls and then DNA was extracted. The molecular investigation was done using 4-primer ARMS PCR and PCR-RFLP methods. Results: Statistical analysis showed that GG genotype [OR= 3.9 (2.1-7.1), P-value< 0.001] and G allele [OR=2.85 (2.07-3.93), P-value< 0.001] frequency of rs231362 polymorphism was significantly different between case and control groups. While rs2237892 polymorphism did not show any differences between the two groups. Conclusion: The result of this study showed that GG genotype and G allele of rs231362 polymorphism can be related to T2DM susceptibility in the population under study.

Effectiveness of Mindfulness-Based Stress Reduction Training on Emotional Expressiveness and Fear of Hypoglycemia in Patients with Type 2 Diabetes

Objective: Diabetes is a common chronic disorder in which there is a high risk of physical complications and psychological problems such as hypoglycemia and inability to express emotions. Psychological interventions can be effective in this regard. The aim of this study was to investigate the effectiveness of mindfulness-based stress reduction (MBSR) training on emotional expressiveness and fear of hypoglycemia (FOH) in patients with type 2 diabetes visiting Yazd Diabetes Center. Materials and Methods: This study was a quasi-experimental study with a pretest and posttest design. The statistical population was all patients with type 2 diabetes visiting Yazd Diabetes Center in 2020. Forty men and women were selected using purposeful sampling and were randomly allocated to case and control groups. The case group received 8 sessions of MBSR training, 2 hours each and the control group received no interventions. Emotional Expressiveness Questionnaire (EEQ) and the Hypoglycemia Fear Survey (HFS) were used to collect data. Results: Results of ANCOVA showed that MBSR training significantly increases emotional expressiveness and its components (P-value< 0.0001) and decreases the fear of hypoglycemia (P-value< 0.0001) in patients with type 2 diabetes (P-value< 0.035). Conclusion: Based on the results of the study, MBSR training could improve emotional expressiveness, prevent hypoglycemia and reduce the fear of it.

The Effectiveness of Logotherapy Training on Psychological Well-Being and Life Expectancy in Patients with Diabetes Type 2

Objective: In daily life, logotherapy is one of the skills that can be taught to increase the levels of psychological well-being and hope. The purpose of this study was to evaluate the effectiveness of meaningful psychological skills in promoting psychological well-being and life expectancy in patients with type 2 diabetes in the city of Salas city of Kermanshah province. Materials and Methods: The present study is an experiment with a pre-test-post-test design with a control group. The statistical population of the study was all patients with type 2 diabetes in Salas city of Kermanshah province. Thirty people were randomly divided into two groups of 15 experimental and controlled by sampling. After that, the experimental group was trained in logotherapy skills and meaning for 2 months in a total of 8 sessions of 90 minutes. After completing the training and after one month, the post-test was performed simultaneously and under the same conditions for both experimental and control groups. Data were analyzed by multivariate analysis of covariance (MANCOVA) with SPSS 18 software. Results: Results of analysis of covariance on post-test means, there is a significant difference between logotherapy and control groups in psychological well-being and life expectancy variables (P-value< 0.001). The value of squares indicates that about 77% of the variance of the well-being score and about 81% of the variance of the life expectancy score is explained by the difference between the two methods. Conclusion: Logotherapy training is effective on psychological well-being and life expectancy of diabetic patients.

Evaluation of the of Positive Psychology Training Effectiveness on Rumination and Depression in Women with Type 2 Diabetes

Objective: Preventing chronic diseases is important for public health. The aim of this study was evaluation the effectiveness of positive psychology (PP) training on rumination and depression in women with type 2 diabetes mellitus (T2DM). Materials and Methods: This research was a quasi-experimental design and a pre-test-post-test with a control group. The statistical population of the present study consisted of women with T2DM. By referring to the medical centers of regions 7, 4, 11, and 16 in Tehran, 30 people were selected by purposive sampling method. Data collection tools in this study were two standard questionnaires of Nolen-Hoeksema and Beck Depression (1978) and positive educational training and guidance protocol. Data were analyzed using SPSS ver. 21. Results: The results showed that PP training has a positive and significant effect on decrease rumination in women with T2DM (Pvalue= 0.001). Also, PP training has a positive and significant effect on reducing depression in women with T2DM (P-value= 0.002). The PP training had a greater effect on rumination in women with T2DM than depression. Conclusion: Positive training in psychology has an impact on rumination and depression in women with T2DM, according to the findings. The findings of the study show that therapeutic intervention for rumination and stress is subordinated to the health network's planned working processes.