The main steam stop valve (MSSV) is installed in the main steam line in thermal and nuclear power plants. The MSSV is a safety valve that instantaneously shuts off the steam flowing into the steam turbine in an emergency. However, as high-speed steam flow goes through the MSSV during even the rated operation, acoustic sound or noise is generated in the MSSV. Moreover, there is a possibility that flow-induced acoustic resonance occurs in the MSSV. Flow-induced acoustic resonance must be suppressed to decrease the sound noise. Reducing the pressure loss of the MSSV is also an important issue that cannot be neglected with respect to the plant thermal efficiency. Therefore, we have developed the MSSV which can suppress the flow-induced acoustic resonance and its pressure loss. To develop this MSSV, we conducted scale air tests and computational fluid dynamics (CFD) analyses that are described in this paper. Mach and Strouhal number of the test conditions were the same as those of an actual plant. Reynolds number was sufficiently large to obtain the developed turbulent flow. An unsteady compressible CFD analysis was also conducted using large eddy simulation as a turbulence model. We developed new tilted triangular tabs and installed them in the MSSV to suppress the intense vortex generation and pressure loss. As a result, the sound noise due to the flow-induced acoustic resonance was completely attenuated and pressure loss was reduced compared to the case using the current tilted tabs. CFD results also showed that the tilted triangular tabs could suppress the generation of intense vortexes and the flow-induced acoustic resonance.