Influence of phosphate on the formation of the indole alkaloids and phenolic compounds in cell suspension cultures of Catharanthus roseus I. Comparison of enzyme activities and product accumulation

1983 ◽  
Vol 2 (4) ◽  
pp. 333-340 ◽  
Author(s):  
K-H. Knobloch ◽  
J. Berlin
Keyword(s):  
Phenolic Compounds ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Enzyme Activities ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Product Accumulation

Influence of Medium Composition on the Formation of Secondary Compounds in Cell Suspension Cultures of Catharanthus roseus (L.) G. Don

1980 ◽  
Vol 35 (7-8) ◽  
pp. 551-556 ◽  
Author(s):  
K.-H. Knobloch ◽  
J. Berlin
Keyword(s):  
Phenolic Compounds ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Culture Media ◽  
Sucrose Solution ◽  
Cell Suspension Cultures ◽  
Secondary Compounds ◽  
Suspension Cultures ◽  
Medium Composition ◽  
High Concentrations

Cell suspension cultures of Catharanthus roseus have been subjected to various media condi­tions in order to stimulate the formation of indole alkaloids. High ajmalicine contents (up to 0.5 mg/g cell fresh weight) were achieved by transferring 2-week-old cell suspensions to a 10-fold volume of a 8% sucrose solution. The alkaloid accumulation started two days after the transfer and reached a plateau after ten days. Furthermore an enhanced level of phenolic compounds was found, whereas growth of the culture was low. The accumulation of both, alkaloids and poly­phenols was stimulated by high concentrations of sucrose and low concentrations of nitrogen con­taining salts and phosphate. When these minerals were added to the sucrose solution in con­centrations commonly used for cell culture media, the accumulation of alkaloids and phenolic compounds was largely suppressed.

The relationship between the accumulation of a 28 kd polypeptide and that of indole alkaloids in Catharanthus roseus cell suspension cultures

1997 ◽  
Vol 150 (4) ◽  
pp. 452-457 ◽  
Author(s):  
Sabine Carpin ◽  
Lazhar Ouelhazi ◽  
Mohamed Filali ◽  
Jean-Claude Chénieux ◽  
Marc Rideau ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
The Relationship

Synthesis of dimeric indole alkaloids by cell free extracts from cell suspension cultures of Catharanthus roseus

1988 ◽  
Vol 27 (5) ◽  
pp. 1355-1359 ◽  
Author(s):  
Masanaru Misawa ◽  
Tsuyoshi Endo ◽  
Anne Goodbody ◽  
John Vukovic ◽  
Clint Chapplet ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Suspension Cultures
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