Clonal propagation of Camptotheca acuminata through shoot bud culture

1996 ◽  
Vol 44 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Ashok K. Jain ◽  
Craig L. Nessler
Keyword(s):  
Clonal Propagation ◽  
Camptotheca Acuminata ◽  
Shoot Bud ◽  
Shoot Bud Culture

scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals IN VITRO REGENERATION OF LITCHI (Litchi chinensis SONN.) THROUGH SHOOT BUD CULTURE

2020 ◽  
pp. 141-146
Author(s):  
Safeer ud Din ◽  
◽  
Waqar Shafqat
Keyword(s):  
In Vitro Regeneration ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Shoot Bud ◽  
Full Strength ◽  
In Vitro Shoots ◽  
Survival Frequency ◽  
Shoot Bud Culture ◽  
Positive Effect

Attempts were made to develop protocol for in vitro regeneration of litchi through axillary shoot bud cultures. The problem of microbial contamination, phenolic exudation and media browning was controlled up to some extend by pretreatment and rapid subculturing. A high frequency (51 %) shoot induction and differentiation was obtained in litchi Gola variety axillary explants on MS medium containing GA3 and BAP (1 mg/l), Kin (2 mg/l). In vitro raised shoots better proliferated in medium containing 2 mg/l BAP. BAP had positive effect on multiplication and growth of shoots but higher concentration than 2 mg/l reduced growth. Maximum rooting frequency (66.67%) with healthier roots was obtained in shoots cultured on full strength MS medium supplemented with IBA (2 mg/l). Plants with well-developed roots were transferred to soil with survival frequency of 57%. A combination of BAP and GA3 (1 mg/l), KIN (2 mg/l) was effective in establishment of cultures. While BAP (2 mg/l) and KIN (3 mg/l) was good for better flourishing in vitro raised shoots. 6-benzylaminepurine had positive effect on multiplication and growth of in vitro shoots but concentration exceeding 2 mg/l decreased growth. Full strength MS medium containing 2 mg/l IBA under dark condition promoted rooting in in vitro raised shoots. The protocol established could prove advantageous to the horticulturists and the industry for developing trees true to the parental type.

scholarly journals Micropropagation of Artocarpus lakoocha Roxb (Monkey jack) through Shoot Bud Culture

2014 ◽  
Author(s):  
Geetika Pant ◽  
Umesh Kabade
Keyword(s):  
Shoot Bud ◽  
Shoot Bud Culture

Intra-inflorescence sex expression and allocation in Camptotheca acuminata

2011 ◽  
Vol 35 (12) ◽  
pp. 1290-1299 ◽  
Author(s):  
Shen-Lin XU ◽  
Wen-Zhe LIU
Keyword(s):  
Sex Expression ◽  
Camptotheca Acuminata

scholarly journals Whole genome resequencing and custom genotyping unveil clonal lineages in ‘Malbec’ grapevines (Vitis vinifera L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luciano Calderón ◽  
Nuria Mauri ◽  
Claudio Muñoz ◽  
Pablo Carbonell-Bejerano ◽  
Laura Bree ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Somatic Mutations ◽  
Clonal Propagation ◽  
Variant Calling ◽  
Vitis Vinifera L ◽  
Whole Genome ◽  
Single Nucleotide Variants ◽  
Genome Resequencing ◽  
Diversity Pattern ◽  
Whole Genome Resequencing

AbstractGrapevine cultivars are clonally propagated to preserve their varietal attributes. However, genetic variations accumulate due to the occurrence of somatic mutations. This process is anthropically influenced through plant transportation, clonal propagation and selection. Malbec is a cultivar that is well-appreciated for the elaboration of red wine. It originated in Southwestern France and was introduced in Argentina during the 1850s. In order to study the clonal genetic diversity of Malbec grapevines, we generated whole-genome resequencing data for four accessions with different clonal propagation records. A stringent variant calling procedure was established to identify reliable polymorphisms among the analyzed accessions. The latter procedure retrieved 941 single nucleotide variants (SNVs). A reduced set of the detected SNVs was corroborated through Sanger sequencing, and employed to custom-design a genotyping experiment. We successfully genotyped 214 Malbec accessions using 41 SNVs, and identified 14 genotypes that clustered in two genetically divergent clonal lineages. These lineages were associated with the time span of clonal propagation of the analyzed accessions in Argentina and Europe. Our results show the usefulness of this approach for the study of the scarce intra-cultivar genetic diversity in grapevines. We also provide evidence on how human actions might have driven the accumulation of different somatic mutations, ultimately shaping the Malbec genetic diversity pattern.

Nitrogen and litter addition decreased sexual reproduction and increased clonal propagation in grasslands

Oecologia ◽  
2021 ◽  
Vol 195 (1) ◽  
pp. 131-144
Author(s):  
Zimeng Li ◽  
Jinfeng Wu ◽  
Qing Han ◽  
Kunyan Nie ◽  
Jiani Xie ◽  
...  
Keyword(s):  
Sexual Reproduction ◽  
Clonal Propagation ◽  
Litter Addition

Genome-wide identification and analysis of AP2/ERF transcription factors related to camptothecin biosynthesis in Camptotheca acuminata

2020 ◽  
Vol 18 (8) ◽  
pp. 582-593
Author(s):  
Ya-Ting HU ◽  
Zhi-Chao XU ◽  
Ya TIAN ◽  
Ran-Ran GAO ◽  
Ai-Jia JI ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Camptotheca Acuminata ◽  
Genome Wide ◽  
Erf Transcription Factors

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants

2003 ◽  
Vol 39 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Surya Prakash ◽  
Veena Agrawal ◽  
S. C. Gupta
Keyword(s):  
Clonal Propagation ◽  
Male And Female ◽  
In Vitro Clonal Propagation
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