This study characterized the lignin peroxidase (LiP) activity of soil via an enzyme assay to determine the reaction rates and activation energies for 5 wt%, 10 wt%, 15 wt%, and 20 wt% lignin loads in urea crosslinked starch biocomposites. The results revealed that a mixed mode of LiP inhibition occurred after the soil was mixed with these biocomposites with different loads of lignin. Loading of lignin at 5 wt% and 10 wt% lignin resulted in higher values of catalytic activity of LiP: -39.58 and 49.14 µM h-1 g-1 soil, respectively. In comparison, with higher loading of lignin at 15 wt% and 20 wt%, decreases in the catalytic activity of LiP were found and were 28.72 to 37.25 µM h-1 g-1 soil, respectively. The activation energy of LiP increased approximately 1.11- to 1.22-fold when 15 and 20 wt% of lignin was loaded in biocomposites. Research findings established the possibility of unfavorable binding of the LiP to lignin with an increase in the load of lignin, possibly due to the complex structure of intact lignin and presence of inhibitory biodegradation products of lignin accumulates during lignin biodegradation in biocomposites. It was concluded that higher lignin contents (15 wt% and 20 wt%) were effective in reducing the activity of the soil LiP. Hence, higher lignin content possibly protects against losses of lignin, while acting as a filler in the formulation of biocomposites.
NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase from Phanerochaete chrysosporium. Comparison with horseradish peroxidase
