Distribution of type I collagen, type II collagen and PNA binding glycoconjugates during chondrogenesis of three distinct embryonic cartilages

1992 ◽  
Vol 186 (3) ◽  
Author(s):  
Yasuyuki Sasano ◽  
Itaru Mizoguchi ◽  
Manabu Kagayama ◽  
Lillian Shum ◽  
Pablo Bringas ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Collagen Type ◽  
Type Ii Collagen ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii

β1 Integrins Mediate Chondrocyte Interaction with Type I Collagen, Type II Collagen, and Fibronectin

1993 ◽  
Vol 205 (2) ◽  
pp. 276-285 ◽  
Author(s):  
Motomi Enomoto ◽  
Phoebe S. Leboy ◽  
A.Sue Menko ◽  
David Boettiger
Keyword(s):  
Type I Collagen ◽  
Collagen Type ◽  
Type Ii Collagen ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Β1 Integrins

scholarly journals Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies

2018 ◽  
Vol 9 ◽  
pp. 204173141878982 ◽  
Author(s):  
Elisa Costa ◽  
Cristina González-García ◽  
José Luis Gómez Ribelles ◽  
Manuel Salmerón-Sánchez
Keyword(s):  
Lactic Acid ◽  
Type I Collagen ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Collagen Type I ◽  
Cell Phenotype ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Chondrocyte Phenotype

Articular chondrocytes are difficult to grow, as they lose their characteristic phenotype following expansion on standard tissue culture plates. Here, we show that culturing them on surfaces of poly(L-lactic acid) of well-defined microtopography allows expansion and maintenance of characteristic chondrogenic markers. We investigated the dynamics of human chondrocyte dedifferentiation on the different poly(L-lactic acid) microtopographies by the expression of collagen type I, collagen type II and aggrecan at different culture times. When seeded on poly(L-lactic acid), chondrocytes maintained their characteristic hyaline phenotype up to 7 days, which allowed to expand the initial cell population approximately six times without cell dedifferentiation. Maintenance of cell phenotype was afterwards correlated to cell adhesion on the different substrates. Chondrocytes adhesion occurs via the α5 β1 integrin on poly(L-lactic acid), suggesting cell–fibronectin interactions. However, α2 β1 integrin is mainly expressed on the control substrate after 1 day of culture, and the characteristic chondrocytic markers are lost (collagen type II expression is overcome by the synthesis of collagen type I). Expanding chondrocytes on poly(L-lactic acid) might be an effective solution to prevent dedifferentiation and improving the number of cells needed for autologous chondrocyte transplantation.

Type VI collagen expression is upregulated in the early events of chondrocyte differentiation

Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 245-251
Author(s):  
R. Quarto ◽  
B. Dozin ◽  
P. Bonaldo ◽  
R. Cancedda ◽  
A. Colombatti
Keyword(s):  
Suspension Culture ◽  
Type I Collagen ◽  
Type Ii Collagen ◽  
Type I ◽  
Type Ii ◽  
Type Vi Collagen ◽  
Full Spectrum ◽  
Collagen Expression ◽  
Hypertrophic Chondrocyte

Dedifferentiated chondrocytes cultured adherent to the substratum proliferate and synthesize large amounts of type I collagen but when transferred to suspension culture they decrease proliferation, resume the chondrogenic phenotype and the synthesis of type II collagen, and continue their maturation to hypertrophic chondrocyte (Castagnola et al., 1986, J. Cell Biol. 102, 2310–2317). In this report, we describe the developmentally regulated expression of type VI collagen in vitro in differentiating avian chondrocytes. Type VI collagen mRNA is barely detectable in dedifferentiated chondrocytes as long as the attachment to the substratum is maintained, but increases very rapidly upon passage of the cells into suspension culture reaching a peak after 48 hours and declining after 5–6 days of suspension culture. The first evidence of a rise in the mRNA steady-state levels is obtained already at 6 hours for the alpha 3(VI) chain. Immunoprecipitation of metabolically labeled cells with type VI collagen antibodies reveals that the early mRNA rise is paralleled by an increased secretion of type VI collagen in cell media. Induction of type VI collagen is not the consequence of trypsin treatment of dedifferentiated cells since exposure to the actin-disrupting drug cytochalasin or detachment of the cells by mechanical procedures has similar effects. In 13-day-old chicken embryo tibiae, where the full spectrum of the chondrogenic differentiation process is represented, expression of type VI collagen is restricted to the articular cartilage where chondrocytes developmental stage is comparable to stage I (high levels of type II collagen expression).(ABSTRACT TRUNCATED AT 250 WORDS)

In situ hybridization reveals differential spatial distribution of mRNAs for type I and type II collagen in the chick limb bud

Development ◽  
1988 ◽  
Vol 103 (1) ◽  
pp. 111-118 ◽  
Author(s):  
C.J. Devlin ◽  
P.M. Brickell ◽  
E.R. Taylor ◽  
A. Hornbruch ◽  
R.K. Craig ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Limb Development ◽  
Type I Collagen ◽  
Type Ii Collagen ◽  
Limb Bud ◽  
Type I ◽  
Type Ii ◽  
Mrna Accumulation ◽  
Rna Probes

During limb development, type I collagen disappears from the region where cartilage develops and synthesis of type II collagen, which is characteristic of cartilage, begins. In situ hybridization using antisense RNA probes was used to investigate the spatial localization of type I and type II collagen mRNAs. The distribution of the mRNA for type II collagen corresponded well with the pattern of type II collagen synthesis, suggesting control at the level of transcription and mRNA accumulation. In contrast, the pattern of mRNA for type I collagen remained more or less uniform and did not correspond with the synthesis of the protein, suggesting control primarily at the level of translation or of RNA processing.

Limb bud mesenchyme cultured under tensile strain remodel collagen type I tubes to produce fibrillar collagen type II

Biorheology ◽  
2009 ◽  
Vol 46 (6) ◽  
pp. 439-450 ◽  
Author(s):  
Jennifer R. Amos ◽  
Shigeng Li ◽  
Michael Yost ◽  
Harry Phloen ◽  
Jay D. Potts
Keyword(s):  
Tensile Strain ◽  
Collagen Type ◽  
Collagen Type I ◽  
Limb Bud ◽  
Type I ◽  
Fibrillar Collagen ◽  
Type Ii ◽  
Collagen Type Ii

Limb bud mesenchyme cultured under tensile strain remodel collagen type I tubes to produce fibrillar collagen type II

Biorheology ◽  
2010 ◽  
Vol 47 (2) ◽  
pp. 163-163
Author(s):  
Jennifer R. Amos ◽  
Shigeng Li ◽  
Michael Yost ◽  
Harry Phloen ◽  
Jay D. Potts
Keyword(s):  
Tensile Strain ◽  
Collagen Type ◽  
Collagen Type I ◽  
Limb Bud ◽  
Type I ◽  
Fibrillar Collagen ◽  
Type Ii ◽  
Collagen Type Ii

Enhanced Early Tissue Regeneration after Matrix-Assisted Autologous Mesenchymal Stem Cell Transplantation in Full Thickness Chondral Defects in a Minipig Model

2009 ◽  
Vol 18 (8) ◽  
pp. 923-932 ◽  
Author(s):  
Martin Jung ◽  
Balazs Kaszap ◽  
Anna Redöhl ◽  
Eric Steck ◽  
Steffen Breusch ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Collagen Type ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Histomorphometric Analysis ◽  
Tissue Quality ◽  
Repair Tissue ◽  
Adult Mesenchymal Stem Cells ◽  
Defect Area

Adult mesenchymal stem cells (MSCs) are an attractive cell source for new treatment strategies in regenerative medicine. This study investigated the potential effect of matrix assisted MSC transplantation for articular cartilage regeneration in a large-animal model 8 weeks postoperatively. MSCs from bone marrow aspirates of eight Goettingen minipigs were isolated and expanded prior to surgery. Articular cartilage defects of 5.4 mm were created bilaterally in the medial patellar groove without penetrating the subchondral bone plate. Defects were either left empty ( n = 4), covered with a collagen type I/III membrane ( n = 6) or additionally treated with autologous MSC transplantation (2 × 106; n = 6). After 8 weeks animals were euthanized and the defect area was assessed for its gross appearance. Histomorphological analysis of the repair tissue included semiquantitative scoring (O'Driscoll score) and quantitative histomorphometric analysis for its glycosaminoglycan (GAG) and collagen type II content. All membranes were found to cover the defect area 8 weeks postoperatively. Median defect filling was 115.8% (membrane), 117.8% (empty), and 100.4% (MSC), respectively (not significant). Histomorphological scoring revealed significantly higher values in MSC-treated defects (median 16.5) when compared to membrane treatment (median 9.5) or empty defects (median 11.5; p = 0.015 and p = 0.038). Histomorphometric analysis showed larger GAG/collagen type II-positive areas in the MSC-treated group (median 24.6%/29.5% of regeneration tissue) compared to 13.6%/33.1% (empty defects) and 1.7%/6.2% (membrane group; p = 0.066). Cell distribution was more homogeneous in MSC compared to membrane-only group, where cells were found mainly near the subchondral zone. In conclusion, autologous matrix-assisted MSC transplantation significantly increased the histomorphological repair tissue quality during early articular cartilage defect repair and resulted in higher GAG/collagen type II-positive cross-sectional areas of the regenerated tissue.

Optimization of Histoprocessing Methods to Detect Glycosaminoglycan, Collagen Type II, and Collagen Type I in Decalcified Rabbit Osteochondral Sections

2005 ◽  
Vol 28 (3) ◽  
pp. 165-175 ◽  
Author(s):  
Anik Chevrier ◽  
Evgeny Rossomacha ◽  
Michael D. Buschmann ◽  
Caroline D. Hoemann
Keyword(s):  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii

Characteristics of cellular immune responses to collagen type I or collagen type II

1986 ◽  
Vol 100 (2) ◽  
pp. 314-330 ◽  
Author(s):  
L. Butler ◽  
B. Simmons ◽  
J. Zimmermann ◽  
P. DeRiso ◽  
K. Phadke
Keyword(s):  
Immune Responses ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Cellular Immune Responses ◽  
Cellular Immune
Sign in / Sign up

Export Citation Format

Share Document