Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes

1991 ◽  
Vol 3 (3) ◽  
pp. 271 ◽  
Author(s):  
Julie E. Gray ◽  
Bruce A. McClure ◽  
Ingrid Bonig ◽  
Marilyn A. Anderson ◽  
Adrienne E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

scholarly journals Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

1991 ◽  
pp. 271-283 ◽  
Author(s):  
J. E. Gray ◽  
B. A. McClure ◽  
I. Bonig ◽  
M. A. Anderson ◽  
A. E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Sequence Variability of Three Alleles of the Self-Incompatibility Gene of Nicotiana alata

1989 ◽  
Vol 1 (5) ◽  
pp. 483 ◽  
Author(s):  
Marilyn A. Anderson ◽  
Geoffrey I. McFadden ◽  
Robert Bernatzky ◽  
Angela Atkinson ◽  
Timothy Orpin ◽  
...  
Keyword(s):  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility ◽  
Sequence Variability

An in vitro assay for assessing the effects of growth factors on Nicotiana alata pollen tubes

1997 ◽  
Vol 10 (6) ◽  
pp. 351-357 ◽  
Author(s):  
W. M. Lush ◽  
A. S. Opat ◽  
F. Nie ◽  
Adrienne E. Clarke
Keyword(s):  
Growth Factors ◽  
Pollen Tubes ◽  
In Vitro Assay ◽  
Nicotiana Alata ◽  
Vitro Assay

scholarly journals Alterations in the Actin Cytoskeleton of Pollen Tubes Are Induced by the Self-Incompatibility Reaction in Papaver rhoeas

2000 ◽  
Vol 12 (7) ◽  
pp. 1239-1251 ◽  
Author(s):  
Anja Geitmann ◽  
Benjamin N. Snowman ◽  
Anne Mie C. Emons ◽  
Vernonica E. Franklin-Tong
Keyword(s):  
Actin Cytoskeleton ◽  
Pollen Tubes ◽  
The Self ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatibility Reaction

scholarly journals Incompatibility Alleles in Portuguese Hazelnut Landraces

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Sandra Martins ◽  
Mercè Rovira ◽  
Ana Paula Silva ◽  
Valdemar Carnide
Keyword(s):  
Higher Plants ◽  
Pollen Tubes ◽  
The Self ◽  
Single Locus ◽  
Self Incompatibility ◽  
Multiple Alleles ◽  
Breeding Programmes ◽  
Incompatibility Alleles ◽  
Related Individuals ◽  
New Cultivars

In many higher plants, selffertilization and genetically related individuals are prevented by pollen-stigma incompatibility. In the genus Corylus, incompatibility is of the sporophytic type and controlled by a single locus with multiple alleles. The objective of this study is to identify the S-alleles present in a collection of Portuguese landraces in order to select the most appropriate landraces for establishment of future orchards and for breeding programmes. Ten major Portuguese hazelnut landraces were submitted to controlled pollinations in the field, with 18 genotypes whose S-alleles are known. The pollen tubes were observed at 100X under a florescence microscope to evaluate their development. Three landraces were revealed to have S2 allele, two have S5, and four have one of the S3, S5, S10, and S18 alleles. One landrace was compatible with the 18 S-alleles tested and for two landraces, it was possible to identify both alleles. The information of the self-incompatibility relationship between these old cultivars is obviously useful for selecting the most suitable pollinators for planning new orchards and for new cultivars development.

scholarly journals Effect on in Vitro Pollen Growth of an Isolated Style Glycoprotein Associated with Self-Incompatibility in Nicotiana alata

1989 ◽  
Vol 89 (1) ◽  
pp. 360-367 ◽  
Author(s):  
Philip J. Harris ◽  
Jodie A. Weinhandl ◽  
Adrienne E. Clarke
Keyword(s):  
Nicotiana Alata ◽  
Self Incompatibility ◽  
Pollen Growth

scholarly journals Ratio-imaging of Ca2+i in the self-incompatibility response in pollen tubes of Papaver rhoeas

1997 ◽  
Vol 12 (6) ◽  
pp. 1375-1386 ◽  
Author(s):  
Vernonica E. Franklin-Tong ◽  
Grant Hackett ◽  
Peter K. Hepler
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Ratio Imaging

scholarly journals Two Self-Incompatibility Sites Occur Simultaneously in the Same Acianthera Species (Orchidaceae, Pleurothallidinae)

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1758
Author(s):  
Mariana Oliveira Duarte ◽  
Denise Maria Trombert Oliveira ◽  
Eduardo Leite Borba
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Main Reaction ◽  
Reaction Site ◽  
Self Incompatibility ◽  
Incompatible Pollen ◽  
Plant Groups ◽  
Transmission Electron ◽  
Incompatibility System ◽  
Cytological Characteristics

In most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of Acianthera, incompatible pollen tubes with anomalous morphology reach the ovary, as those are obstructed in the column. We investigated if a distinct self-incompatibility (SI) system is acting on the ovary of A. johannensis, which is a species with partial self-incompatibility, contrasting with a full SI species, A. fabiobarrosii. We analyzed the morphology and development of pollen tubes in the column, ovary, and fruit using light, epifluorescence, and transmission electron microscopy. Our results show that the main reaction site in A. johannensis is in the stylar canal inside the column, which was also recorded in A. fabiobarrosii. Morphological and cytological characteristics of the pollen tubes with obstructed growth in the column indicated a process of programmed cell death in these tubes, showing a possible GSI reaction. In addition, partially self-incompatible individuals of A. johannensis exhibit a second SI site in the ovary. We suggest that this self-incompatibility site in the ovary is only an extension of GSI that acts in the column, differing from the typical late-acting self-incompatibility system recorded in other plant groups.

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