Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil

1986 ◽  
Vol 245 (2) ◽  
Author(s):  
M. Didier ◽  
M. Harandi ◽  
M. Aguera ◽  
B. Bancel ◽  
M. Tardy ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Subcommissural Organ ◽  
Protein S ◽  
Immunocytochemical Staining ◽  
Gfa Protein ◽  
S 100

scholarly journals Immunohistochemical Characterization of Canine Transmissible Venereal Tumor

1996 ◽  
Vol 33 (3) ◽  
pp. 257-263 ◽  
Author(s):  
E. Mozos ◽  
A. Méndez ◽  
J. C. Gómez-Villamandos ◽  
J. Martín de las Mulas ◽  
J. Pérez
Keyword(s):  
Light Chain ◽  
B Lymphocyte ◽  
Protein S ◽  
Lambda Light Chain ◽  
Formalin Fixed Paraffin ◽  
S 100 ◽  
Formalin Fixed Paraffin Embedded ◽  
Progressive Growth ◽  
Staining Techniques ◽  
Alpha 1 Antitrypsin

The collective immunohistochemical expression of human lysozyme, human alpha-1-antitrypsin, human CD3 antigen, calf vimentin, human keratins, human lambda light chains, canine immunoglobulins IgG, IgM, and bovine protein S-100 has been analyzed on formalin-fixed, paraffin-embedded tissue sections of 25 spontaneous canine transmissible venereal tumors (CTVT) from both genital and extragenital locations using the avidin-biotin-peroxidase complex technique. Lysozyme immunoreactivity was detected in 10/25 CTVT, alpha-1-antitrypsin in 14/25 CTVT, and vimentin in 25/25 CTVT. All CTVT cells were negative to keratins 5 + 8 of the Moll catalogue (RCK-102), S-100 protein, lambda light-chain immunoglobulins, IgG, IgM, and CD3 antigen. The intratumoral T- and B-lymphocyte infiltrate was differentiated using CD3 antigen, lambda light-chain immunoglobulins, IgG, and IgM, and this technique could be useful to evaluate the regressive or progressive growth stage of venereal tumors. Our findings support the hypothesis of a histiocytic immunophenotype for CTVT, and these staining techniques could be used in the differential diagnosis with lymphomas.

scholarly journals Locally advanced malignant solitary fibrous tumour successfully treated with conversion chemotherapy, operation and postoperative radiotherapy: a case report

2021 ◽  
Vol 49 (3) ◽  
pp. 030006052199694
Author(s):  
Zhi-Ke Li ◽  
Jie Liu ◽  
Chen Chen ◽  
Ke-Yi Yang ◽  
Yao-Tiao Deng ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Postoperative Radiotherapy ◽  
Locally Advanced ◽  
Rna Binding Protein ◽  
Disease Free Survival ◽  
Protein S ◽  
Solitary Fibrous Tumour ◽  
Final Pathology ◽  
S 100

Preoperative diagnosis of solitary fibrous tumour (SFT) may not provide a complete tumour picture and may be inaccurate. There is no standard treatment for locally advanced or metastasised malignant SFT (MSFT). Here, the case of a 17-year-old male patient with final pathology diagnosis of MSFT is reported. Preoperative biopsy pathology results suggested an Ewing sarcoma that was positive for CD99 antigen, vimentin, friend leukaemia integration 1 transcription factor, apoptosis regulator Bcl-2, and synaptophysin; and negative for CD34 antigen, S-100 protein (S-100), smooth muscle antigen, cytokeratin, and Wilms tumour 1 associated protein. The Ki67 positive rate was 8%, so the patient initially received eight cycles of conversion chemotherapy (vincristine, etoposide, ifosfamide and pirarubicin for one cycle, and vincristine, doxorubicin, and cyclophosphamide/ifosfamide and etoposide for 7 cycles in total). The tumour shrunk significantly and was surgically removed. The final pathology diagnosis was MSFT that was positive for CD99 and signal transducer and activator of transcription 6, and negative for CD34, tumour protein 63, S-100, desmin, and epithelial membrane antigen. Fluorescence in situ hybridization showed no gene translocation in EWS RNA binding protein 1, SS18 subunit of BAF chromatin remodelling complex or FUS RNA binding protein. The patient finally accepted adjuvant radiotherapy of 5600 cGy. Disease-free survival has been > 1 year, with no recurrence or metastasis detected to date. MSFT is rare and treatment for locally advanced or metastatic MSFT remains controversial. The efficacy of the present therapeutic strategy requires further research.

Protein S-100 and neuron-specific enolase serum levels remain unaffected by electroconvulsive therapy in patients with depression

2014 ◽  
Vol 121 (11) ◽  
pp. 1411-1415 ◽  
Author(s):  
Laura Kranaster ◽  
Christoph Janke ◽  
Sonani Mindt ◽  
Michael Neumaier ◽  
Alexander Sartorius
Keyword(s):  
Electroconvulsive Therapy ◽  
Neuron Specific Enolase ◽  
Protein S ◽  
Serum Levels ◽  
S 100

Evaluation of Serum Protein S-100 as a New Melanoma Marker

1997 ◽  
pp. 1136-1144
Author(s):  
G. Ghanem ◽  
B. Loir ◽  
J. C. Pector ◽  
P. Ewalenko ◽  
J. Kerger ◽  
...  
Keyword(s):  
Serum Protein ◽  
Protein S ◽  
S 100

scholarly journals Retrospective canine skin peripheral nerve sheath tumors data with emphasis on histologic, immunohistochemical and prognostic factors

2015 ◽  
Vol 35 (12) ◽  
pp. 965-974 ◽  
Author(s):  
Gisele S. Boos ◽  
Daniele M. Bassuino ◽  
Fabiana Wurster ◽  
Neusa B. Castro ◽  
Tatiane T.N. Watanabe ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Protein S ◽  
Skin Neoplasms ◽  
Tumor Location ◽  
Benign Tumors ◽  
Nerve Sheath Tumor ◽  
Nerve Sheath Tumors ◽  
Peripheral Nerve Sheath Tumors ◽  
Nerve Sheath ◽  
S 100

Abstract: In this retrospective study was determined the frequency of canine skin peripheral nerve sheath tumors (PNST) in cases diagnosed by the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brazil, between the years 2000 and 2012. The canine profiles, as well as histological, immunohistochemical and prognostic aspects of the tumors were based on 70 samples, comprising 40 females, 29 males and one unspecified sample. Between 2000 and 2012, 2,984 skin tumors of dogs were diagnosed in the SPV-UFRGS, totaling 2.34% of skin neoplasms in dogs. Animals that comprised the largest amount of samples (43%) were those with no breed (SRD), followed by German Shepherds (10%). Females were more affected than males (40/70 - 57% and 29/70 - 41% respectively). Skin PNST of this research showed predominant localization on the limbs (40% in the forelimbs and 29% in the hindlimbs); affecting adult dogs, mostly aged between 8 and 11 years (54%). The samples were routinely processed for hematoxylin and eosin, and were also evaluated by toluidine blue and Masson's trichrome staining, and immunohistochemistry (IHC) anti-vimentin, -S-100, -GFAP, -actin, von Willebrand factor and neurofilament. Anisocytosis and anisokaryosis, mitotic index, intratumoral necrosis, invasion of adjacent tissues, tumor location, local recurrence and metastasis were related to the diagnosis of benign (49/70) or malignant tumor (21/70). The Antoni A histological pattern was observed more frequently in benign tumors. The immunohistochemistry helped to diagnose PNST, and anti-vimentin and anti-protein S-100 showed the highest rates of immunostaining. Throughout statistical analysis of animals with tumor recurrence, it was found that the chance of an animal with a malignant peripheral nerve sheath tumor to develop recurrence is 4.61 times higher than in an animal that had a benign tumor.

Conformational and immunological properties of a bovine brain acidic protein (S-100)

Biochemistry ◽  
1968 ◽  
Vol 7 (2) ◽  
pp. 758-764 ◽  
Author(s):  
Dietrich. Kessler ◽  
Lawrence. Levine ◽  
Gerald D. Fasman
Keyword(s):  
Protein S ◽  
Bovine Brain ◽  
Acidic Protein ◽  
Immunological Properties ◽  
S 100

A Bioinformatic Approach to the Identification of Candidate Genes for the Development of New Cancer Diagnostics

2003 ◽  
Vol 384 (2) ◽  
pp. 321-327 ◽  
Author(s):  
G. Musumarra ◽  
V. Barresi ◽  
D.F. Condorelli ◽  
S. Scirè
Keyword(s):  
Protein S ◽  
Cancer Diagnostics ◽  
Clinical Settings ◽  
Melanoma Metastasis ◽  
Dopachrome Tautomerase ◽  
Ovarian Cells ◽  
S 100 ◽  
Gene Transcripts ◽  
Pca Method ◽  
Bioinformatic Approach

Abstract A multivariate analysis of the National Cancer Institute gene expression database is reported here. The soft independent modelling of a class analogy approach achieved cell line classification according to histological origin. With the PCA method, based on the expression of 9605 genes and ESTs, classification of colon, leukaemia, renal, melanoma and CNS cells could be performed, but not of lung, breast and ovarian cells. Another multivariate procedure, called partial least squares discriminant analysis (PLS-DA), provides bioinformatic clues for the selection of a limited number of gene transcripts most effective in discriminating different tumoral histotypes. Among them it is possible to identify candidates in the development of new diagnostic tests for cancer detection and unknown genes deserving high priority in further studies. In particular, melan-A, acid phosphatase 5, dopachrome tautomerase, S100-β and acid ceramidase were found to be among the most important genes for melanoma. The potential of the present bioinformatic approach is exemplified by its ability to identify differentiation and diagnostic markers already in use in clinical settings, such as protein S-100, a prognostic parameter in patients with metastatic melanoma and a screening marker for melanoma metastasis.

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