Determination of acid-base equilibria of organic pollutants: the steady state fluorescence emission method

1994 ◽  
Vol 26 (4) ◽  
pp. 510-520 ◽  
Author(s):  
M. J. Melo ◽  
E. Melo ◽  
F. Pina
Keyword(s):  
Steady State ◽  
Organic Pollutants ◽  
Fluorescence Emission ◽  
Emission Method ◽  
Acid Base ◽  
Steady State Fluorescence

scholarly journals Determination of Critical Micellar Aggregation Numbers by Steadystate Fluorescence Probe Method

2001 ◽  
Vol 17 (09) ◽  
pp. 828-831 ◽  
Author(s):  
Fang Yun ◽  
◽  
Liu Xue-Feng ◽  
Xia Yong-Mei ◽  
Yang Yang ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorescence Probe ◽  
Probe Method ◽  
Steady State Fluorescence ◽  
Aggregation Numbers

scholarly journals Effect of PAF on polyrnorphonuclear leucocyte plasma membrane polarity: a fluorescence study

1993 ◽  
Vol 2 (2) ◽  
pp. 149-151 ◽  
Author(s):  
A. Kantar ◽  
P. L. Giorgi ◽  
R. Fiorini
Keyword(s):  
Plasma Membrane ◽  
Steady State ◽  
Polymorphonuclear Leukocytes ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Blue Shift ◽  
Fluorescence Study ◽  
Polar Solvents ◽  
Fluorescence Emission Spectra ◽  
Steady State Fluorescence

The effect of PAF on the plasma membrane polarity of polymorphonuclear leukocytes (PMNs) was investigated by measuring the steady-state fluorescence emission spectra of 2-dimethylamino(6-1auroyl) naphthalene (Laurdan), which is known to be incorporated at the hydrophobic-hydrophilic interface of the bilayer, displaying spectral sensitivity to the polarity of its surrounding. Laurdan shows a marked steady-state emission blue-shift in non-polar solvents, with respect to polar solvents. Our results demonstrate that PAF (10−7M) induces a blue shift of the fluorescence emission spectra of Laurdan. These changes are blocked in the presence of the PAF antagonist, L-659,989. Our data indicate that the interaction between PAF and PMNs is accompanied by a decrease in polarity in the hydrophobic-hydrophilic interface of the plasma membrane.

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